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Quantification of autophagy flux in isolated mouse skeletal muscle fibers with overexpression of fluorescent protein mCherry-EGFP-LC3

Evaluation of autophagy flux could be challenging for muscle fibers due to the baseline expression of mCherry-EGFP-LC3 along the Z-line. We established a protocol to overcome this difficulty. We overexpress mChery-EGFP-LC3 in the FDB muscle of an adult mouse via electroporation. Then, we enzymatical...

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Detalles Bibliográficos
Autores principales:	Zhou, Xinyu, Cho, Ju Hwan, Yi, Jianxun, Choi, Kyounghan, Park, Ki Ho, Zhu, Hua, Cai, Chuanxi, Haggard, Erin, Zhou, Jingsong, Ko, Jae-Kyun, Ma, Jianjie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898804/ https://www.ncbi.nlm.nih.gov/pubmed/36856767 http://dx.doi.org/10.1016/j.xpro.2022.101871

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