C. elegans Gene Regulatory Alleles and Reporter Bashing Studies

Gene regulation has been studied in C. elegans for over 30 years. In this analysis of 102 publications, we find that most transcriptional cis-regulatory elements are located within 5,000 bp of the transcription start site. Over 75% of studies conclude that transcriptional elements and 5′UTRs activat...

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Detalles Bibliográficos
Autores principales: Froehlich, Jonathan J, Rajewsky, Nikolaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898812/
https://www.ncbi.nlm.nih.gov/pubmed/36748042
http://dx.doi.org/10.17912/micropub.biology.000709
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author Froehlich, Jonathan J
Rajewsky, Nikolaus
author_facet Froehlich, Jonathan J
Rajewsky, Nikolaus
author_sort Froehlich, Jonathan J
collection PubMed
description Gene regulation has been studied in C. elegans for over 30 years. In this analysis of 102 publications, we find that most transcriptional cis-regulatory elements are located within 5,000 bp of the transcription start site. Over 75% of studies conclude that transcriptional elements and 5′UTRs activate-, while 3′UTRs repress gene expression. While gene regulatory mutations make up less than 0.8% of alleles in forward genetics screens, recent CRISPR-Cas approaches are increasing the number of tested mutations. This work provides a resource of known gene regulatory sequences in C.elegans .
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spelling pubmed-98988122023-02-05 C. elegans Gene Regulatory Alleles and Reporter Bashing Studies Froehlich, Jonathan J Rajewsky, Nikolaus MicroPubl Biol New Finding Gene regulation has been studied in C. elegans for over 30 years. In this analysis of 102 publications, we find that most transcriptional cis-regulatory elements are located within 5,000 bp of the transcription start site. Over 75% of studies conclude that transcriptional elements and 5′UTRs activate-, while 3′UTRs repress gene expression. While gene regulatory mutations make up less than 0.8% of alleles in forward genetics screens, recent CRISPR-Cas approaches are increasing the number of tested mutations. This work provides a resource of known gene regulatory sequences in C.elegans . Caltech Library 2023-01-20 /pmc/articles/PMC9898812/ /pubmed/36748042 http://dx.doi.org/10.17912/micropub.biology.000709 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Froehlich, Jonathan J
Rajewsky, Nikolaus
C. elegans Gene Regulatory Alleles and Reporter Bashing Studies
title C. elegans Gene Regulatory Alleles and Reporter Bashing Studies
title_full C. elegans Gene Regulatory Alleles and Reporter Bashing Studies
title_fullStr C. elegans Gene Regulatory Alleles and Reporter Bashing Studies
title_full_unstemmed C. elegans Gene Regulatory Alleles and Reporter Bashing Studies
title_short C. elegans Gene Regulatory Alleles and Reporter Bashing Studies
title_sort c. elegans gene regulatory alleles and reporter bashing studies
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898812/
https://www.ncbi.nlm.nih.gov/pubmed/36748042
http://dx.doi.org/10.17912/micropub.biology.000709
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AT rajewskynikolaus celegansgeneregulatoryallelesandreporterbashingstudies

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