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Intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors

Knowledge of the histologic type and primary origin of pulmonary tumors is essential when preparing a surgical strategy. Intraoperative diagnosis of hematoxylin and eosin (H&E)‐stained frozen sections is the gold standard, but reliable pathology requires time‐consuming immunohistochemistry (IHC)...

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Autores principales: Imai, Kazuhiro, Nanjo, Hiroshi, Shigeeda, Wataru, Sugai, Tamotsu, Ito, Tomoo, Maniwa, Yoshimasa, Takashima, Shinogu, Saito, Hajime, Yanagawa, Naoki, Tanaka, Yugo, Doi, Takefumi, Hiroshima, Yuko, Nomura, Kyoko, Tanino, Mishie, Tanaka, Shinya, Minamiya, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899630/
https://www.ncbi.nlm.nih.gov/pubmed/36282212
http://dx.doi.org/10.1111/cas.15616
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author Imai, Kazuhiro
Nanjo, Hiroshi
Shigeeda, Wataru
Sugai, Tamotsu
Ito, Tomoo
Maniwa, Yoshimasa
Takashima, Shinogu
Saito, Hajime
Yanagawa, Naoki
Tanaka, Yugo
Doi, Takefumi
Hiroshima, Yuko
Nomura, Kyoko
Tanino, Mishie
Tanaka, Shinya
Minamiya, Yoshihiro
author_facet Imai, Kazuhiro
Nanjo, Hiroshi
Shigeeda, Wataru
Sugai, Tamotsu
Ito, Tomoo
Maniwa, Yoshimasa
Takashima, Shinogu
Saito, Hajime
Yanagawa, Naoki
Tanaka, Yugo
Doi, Takefumi
Hiroshima, Yuko
Nomura, Kyoko
Tanino, Mishie
Tanaka, Shinya
Minamiya, Yoshihiro
author_sort Imai, Kazuhiro
collection PubMed
description Knowledge of the histologic type and primary origin of pulmonary tumors is essential when preparing a surgical strategy. Intraoperative diagnosis of hematoxylin and eosin (H&E)‐stained frozen sections is the gold standard, but reliable pathology requires time‐consuming immunohistochemistry (IHC) to distinguish among histological types/organ origins and to analyze molecular status. The aim of this study was to evaluate the clinical reliability of a new rapid‐IHC technique for intraoperative diagnosis of pulmonary tumors. In total, 169 patients with undiagnosed pulmonary tumors were enrolled in a multicenter prospective observational study. At three institutes, pulmonary tumor samples were collected through core needle biopsy and/or surgery to determine surgical strategies. Using a new device for rapid IHC, we applied a high‐voltage, low‐frequency alternating current (AC) field, which mixes the available antibody as the voltage is switched on/off. Rapid IHC can provide tumor histologic type/origin diagnoses within 20 min, as opposed to the 3–6 h required for conventional IHC. No false diagnoses of malignancy were rendered in any of the cases when using simple H&E staining. With H&E staining alone, the overall definitive diagnosis rate, the rate of defined tumor origin, and the rate of determined histological type were 76.92%, 85.80%, and 90.53%, respectively. When rapid IHC was added, those rates were significantly improved to 88.76%, 94.67%, and 91.72%, respectively. By providing prompt and accurate intraoperative histological/molecular analysis, rapid IHC driven by AC mixing could serve as an effective clinical tool guiding the surgical strategy for undiagnosed pulmonary tumors.
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spelling pubmed-98996302023-02-09 Intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors Imai, Kazuhiro Nanjo, Hiroshi Shigeeda, Wataru Sugai, Tamotsu Ito, Tomoo Maniwa, Yoshimasa Takashima, Shinogu Saito, Hajime Yanagawa, Naoki Tanaka, Yugo Doi, Takefumi Hiroshima, Yuko Nomura, Kyoko Tanino, Mishie Tanaka, Shinya Minamiya, Yoshihiro Cancer Sci Original Articles Knowledge of the histologic type and primary origin of pulmonary tumors is essential when preparing a surgical strategy. Intraoperative diagnosis of hematoxylin and eosin (H&E)‐stained frozen sections is the gold standard, but reliable pathology requires time‐consuming immunohistochemistry (IHC) to distinguish among histological types/organ origins and to analyze molecular status. The aim of this study was to evaluate the clinical reliability of a new rapid‐IHC technique for intraoperative diagnosis of pulmonary tumors. In total, 169 patients with undiagnosed pulmonary tumors were enrolled in a multicenter prospective observational study. At three institutes, pulmonary tumor samples were collected through core needle biopsy and/or surgery to determine surgical strategies. Using a new device for rapid IHC, we applied a high‐voltage, low‐frequency alternating current (AC) field, which mixes the available antibody as the voltage is switched on/off. Rapid IHC can provide tumor histologic type/origin diagnoses within 20 min, as opposed to the 3–6 h required for conventional IHC. No false diagnoses of malignancy were rendered in any of the cases when using simple H&E staining. With H&E staining alone, the overall definitive diagnosis rate, the rate of defined tumor origin, and the rate of determined histological type were 76.92%, 85.80%, and 90.53%, respectively. When rapid IHC was added, those rates were significantly improved to 88.76%, 94.67%, and 91.72%, respectively. By providing prompt and accurate intraoperative histological/molecular analysis, rapid IHC driven by AC mixing could serve as an effective clinical tool guiding the surgical strategy for undiagnosed pulmonary tumors. John Wiley and Sons Inc. 2022-11-10 /pmc/articles/PMC9899630/ /pubmed/36282212 http://dx.doi.org/10.1111/cas.15616 Text en © 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Imai, Kazuhiro
Nanjo, Hiroshi
Shigeeda, Wataru
Sugai, Tamotsu
Ito, Tomoo
Maniwa, Yoshimasa
Takashima, Shinogu
Saito, Hajime
Yanagawa, Naoki
Tanaka, Yugo
Doi, Takefumi
Hiroshima, Yuko
Nomura, Kyoko
Tanino, Mishie
Tanaka, Shinya
Minamiya, Yoshihiro
Intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors
title Intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors
title_full Intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors
title_fullStr Intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors
title_full_unstemmed Intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors
title_short Intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors
title_sort intraoperative rapid immunohistochemistry with noncontact antibody mixing for undiagnosed pulmonary tumors
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899630/
https://www.ncbi.nlm.nih.gov/pubmed/36282212
http://dx.doi.org/10.1111/cas.15616
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