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The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae

Sterol 14α-demethylase catalyzes lanosterol hydroxylation, which is one of the key reactions in the biosynthetic pathway of sterols. There is only one sterol 14α-demethylases gene named Erg11 in Saccharomyces cerevisiae genome. In this study, three sterol 14α-demethylases genes named AoErg11A, AoErg...

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Autores principales: Jin, Qi, Li, Ganghua, Qin, Kunhai, Shang, Yitong, Yan, Huanhuan, Liu, Hongliang, Zeng, Bin, Hu, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899854/
https://www.ncbi.nlm.nih.gov/pubmed/36755574
http://dx.doi.org/10.3389/fgene.2023.1009746
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author Jin, Qi
Li, Ganghua
Qin, Kunhai
Shang, Yitong
Yan, Huanhuan
Liu, Hongliang
Zeng, Bin
Hu, Zhihong
author_facet Jin, Qi
Li, Ganghua
Qin, Kunhai
Shang, Yitong
Yan, Huanhuan
Liu, Hongliang
Zeng, Bin
Hu, Zhihong
author_sort Jin, Qi
collection PubMed
description Sterol 14α-demethylase catalyzes lanosterol hydroxylation, which is one of the key reactions in the biosynthetic pathway of sterols. There is only one sterol 14α-demethylases gene named Erg11 in Saccharomyces cerevisiae genome. In this study, three sterol 14α-demethylases genes named AoErg11A, AoErg11B and AoErg11C were identified in Aspergillus oryzae genome through bioinformatics analysis. The function of these three genes were studied by yeast complementation, and the expression pattern/subcellular localization of these genes/proteins were detected. The results showed that the three AoErg11s were expressed differently at different growth times and under different abiotic stresses. All of the three proteins were located in endoplasmic reticulum. The AoErg11s could not restore the temperature-sensitive phenotype of S. cerevisiae erg11 mutant. Overexpression of the three AoErg11s affected both growth and sporulation, which may be due to the effect of AoErg11s on ergosterol content. Therefore, this study revealed the functions of three AoErg11s and their effects on the growth and ergosterol biosynthesis of A. oryzae, which may contribute to the further understanding of the ergosterol biosynthesis and regulation mechanism in this important filamentous fungus, A. oryzae.
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spelling pubmed-98998542023-02-07 The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae Jin, Qi Li, Ganghua Qin, Kunhai Shang, Yitong Yan, Huanhuan Liu, Hongliang Zeng, Bin Hu, Zhihong Front Genet Genetics Sterol 14α-demethylase catalyzes lanosterol hydroxylation, which is one of the key reactions in the biosynthetic pathway of sterols. There is only one sterol 14α-demethylases gene named Erg11 in Saccharomyces cerevisiae genome. In this study, three sterol 14α-demethylases genes named AoErg11A, AoErg11B and AoErg11C were identified in Aspergillus oryzae genome through bioinformatics analysis. The function of these three genes were studied by yeast complementation, and the expression pattern/subcellular localization of these genes/proteins were detected. The results showed that the three AoErg11s were expressed differently at different growth times and under different abiotic stresses. All of the three proteins were located in endoplasmic reticulum. The AoErg11s could not restore the temperature-sensitive phenotype of S. cerevisiae erg11 mutant. Overexpression of the three AoErg11s affected both growth and sporulation, which may be due to the effect of AoErg11s on ergosterol content. Therefore, this study revealed the functions of three AoErg11s and their effects on the growth and ergosterol biosynthesis of A. oryzae, which may contribute to the further understanding of the ergosterol biosynthesis and regulation mechanism in this important filamentous fungus, A. oryzae. Frontiers Media S.A. 2023-01-23 /pmc/articles/PMC9899854/ /pubmed/36755574 http://dx.doi.org/10.3389/fgene.2023.1009746 Text en Copyright © 2023 Jin, Li, Qin, Shang, Yan, Liu, Zeng and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Jin, Qi
Li, Ganghua
Qin, Kunhai
Shang, Yitong
Yan, Huanhuan
Liu, Hongliang
Zeng, Bin
Hu, Zhihong
The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae
title The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae
title_full The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae
title_fullStr The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae
title_full_unstemmed The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae
title_short The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae
title_sort expression pattern, subcellular localization and function of three sterol 14α-demethylases in aspergillus oryzae
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899854/
https://www.ncbi.nlm.nih.gov/pubmed/36755574
http://dx.doi.org/10.3389/fgene.2023.1009746
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