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Protective effects of liposomes encapsulating ferulic acid against CCl(4)-induced oxidative liver damage in vivo rat model

Antioxidants are useful for the treatment of oxidative stress mediated liver damage. A naturally occurring antioxidant γ-oryzanol is rapidly hydrolyzed to its active hydrophobic metabolite, ferulic acid, inside the body. Limitations associated with the hydrophobicity of ferulic acid can be overcome...

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Autores principales: Ara, Tabassum, Ono, Satoko, Hasan, Mahadi, Ozono, Mizune, Kogure, Kentaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899917/
https://www.ncbi.nlm.nih.gov/pubmed/36777075
http://dx.doi.org/10.3164/jcbn.22-37
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author Ara, Tabassum
Ono, Satoko
Hasan, Mahadi
Ozono, Mizune
Kogure, Kentaro
author_facet Ara, Tabassum
Ono, Satoko
Hasan, Mahadi
Ozono, Mizune
Kogure, Kentaro
author_sort Ara, Tabassum
collection PubMed
description Antioxidants are useful for the treatment of oxidative stress mediated liver damage. A naturally occurring antioxidant γ-oryzanol is rapidly hydrolyzed to its active hydrophobic metabolite, ferulic acid, inside the body. Limitations associated with the hydrophobicity of ferulic acid can be overcome by encapsulating in a liposomal formulation. As intravenously administered nanoparticles (including liposomes) can effectively reach the liver, such systems may be suitable drug delivery carriers to treat liver injury. In this study, we prepared a liposomal formulation of ferulic acid (ferulic-lipo) and examined its effects on liver damage induced by CCl(4). Ferulic-lipo were ~100 nm in size and drug encapsulation efficiency was about 92%. Ferulic-lipo showed potent scavenging efficacy against hydroxyl radical compared to α-tocopherol liposomes. Ferulic-lipo significantly prevented CCl(4)-mediated cytotoxicity in human hepatocarcinoma cells. Furthermore, intravenous administration of ferulic-lipo significantly reduced alanine aminotransferase and aspartate amino transferase levels in a rat model of liver injury. CCl(4)-mediated reactive oxygen species generation in liver was also reduced by intravenous administration of ferulic-lipo. Hepatoprotective effects of ferulic-lipo were demonstrated by histological observation of CCl(4)-induced liver tissue damage. Therefore, ferulic-lipo exhibit potent antioxidative capacity and were suggested to be an effective formulation for prevention of oxidative damage of liver tissue.
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spelling pubmed-98999172023-02-11 Protective effects of liposomes encapsulating ferulic acid against CCl(4)-induced oxidative liver damage in vivo rat model Ara, Tabassum Ono, Satoko Hasan, Mahadi Ozono, Mizune Kogure, Kentaro J Clin Biochem Nutr Original Article Antioxidants are useful for the treatment of oxidative stress mediated liver damage. A naturally occurring antioxidant γ-oryzanol is rapidly hydrolyzed to its active hydrophobic metabolite, ferulic acid, inside the body. Limitations associated with the hydrophobicity of ferulic acid can be overcome by encapsulating in a liposomal formulation. As intravenously administered nanoparticles (including liposomes) can effectively reach the liver, such systems may be suitable drug delivery carriers to treat liver injury. In this study, we prepared a liposomal formulation of ferulic acid (ferulic-lipo) and examined its effects on liver damage induced by CCl(4). Ferulic-lipo were ~100 nm in size and drug encapsulation efficiency was about 92%. Ferulic-lipo showed potent scavenging efficacy against hydroxyl radical compared to α-tocopherol liposomes. Ferulic-lipo significantly prevented CCl(4)-mediated cytotoxicity in human hepatocarcinoma cells. Furthermore, intravenous administration of ferulic-lipo significantly reduced alanine aminotransferase and aspartate amino transferase levels in a rat model of liver injury. CCl(4)-mediated reactive oxygen species generation in liver was also reduced by intravenous administration of ferulic-lipo. Hepatoprotective effects of ferulic-lipo were demonstrated by histological observation of CCl(4)-induced liver tissue damage. Therefore, ferulic-lipo exhibit potent antioxidative capacity and were suggested to be an effective formulation for prevention of oxidative damage of liver tissue. the Society for Free Radical Research Japan 2023-01 2022-10-05 /pmc/articles/PMC9899917/ /pubmed/36777075 http://dx.doi.org/10.3164/jcbn.22-37 Text en Copyright © 2023 JCBN https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Ara, Tabassum
Ono, Satoko
Hasan, Mahadi
Ozono, Mizune
Kogure, Kentaro
Protective effects of liposomes encapsulating ferulic acid against CCl(4)-induced oxidative liver damage in vivo rat model
title Protective effects of liposomes encapsulating ferulic acid against CCl(4)-induced oxidative liver damage in vivo rat model
title_full Protective effects of liposomes encapsulating ferulic acid against CCl(4)-induced oxidative liver damage in vivo rat model
title_fullStr Protective effects of liposomes encapsulating ferulic acid against CCl(4)-induced oxidative liver damage in vivo rat model
title_full_unstemmed Protective effects of liposomes encapsulating ferulic acid against CCl(4)-induced oxidative liver damage in vivo rat model
title_short Protective effects of liposomes encapsulating ferulic acid against CCl(4)-induced oxidative liver damage in vivo rat model
title_sort protective effects of liposomes encapsulating ferulic acid against ccl(4)-induced oxidative liver damage in vivo rat model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899917/
https://www.ncbi.nlm.nih.gov/pubmed/36777075
http://dx.doi.org/10.3164/jcbn.22-37
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