Cargando…

Comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice

BACKGROUND: Zinc (Zn) is an essential cofactor for physiological homeostasis in the body. Zn oxide (ZnO), an inorganic compound that supplies Zn, exists in various sizes, and its bioavailability may vary depending on the size in vivo. However, comparative studies on the nutritional effects of micro-...

Descripción completa

Detalles Bibliográficos
Autores principales: Yoon, Ja-Seon, Nam, Sang Yoon, Lee, Beom Jun, Lee, Hyun Jik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899942/
https://www.ncbi.nlm.nih.gov/pubmed/36560835
http://dx.doi.org/10.4142/jvs.22201
_version_ 1784882743749902336
author Yoon, Ja-Seon
Nam, Sang Yoon
Lee, Beom Jun
Lee, Hyun Jik
author_facet Yoon, Ja-Seon
Nam, Sang Yoon
Lee, Beom Jun
Lee, Hyun Jik
author_sort Yoon, Ja-Seon
collection PubMed
description BACKGROUND: Zinc (Zn) is an essential cofactor for physiological homeostasis in the body. Zn oxide (ZnO), an inorganic compound that supplies Zn, exists in various sizes, and its bioavailability may vary depending on the size in vivo. However, comparative studies on the nutritional effects of micro-sized ZnO (M-ZnO) and nano-sized ZnO (N-ZnO) supplementation on Zn deficiency (ZnD) animal models have not been reported. OBJECTIVES: This study investigated the nutritional bioavailability of N-ZnO and M-ZnO particles in dietary-induced ZnD mice. METHODS: Animals were divided into six experimental groups: normal group, ZnD control group, and four ZnO treatment groups (Nano-Low, Nano-High, Micro-Low, and Micro-High). After ZnD induction, N-ZnO or M-ZnO was administered orally every day for 4 weeks. RESULTS: ZnD-associated clinical signs almost disappeared 7 days after N-ZnO or M-ZnO administration. Serum Zn concentrations were higher in the Nano-High group than in the ZnD and M-ZnO groups on day 7 of ZnO treatment. In the liver and testis, Nano-Low and Nano-High groups showed significantly higher Zn concentrations than the other groups after 14-day treatment. ZnO supplementation increased Mt-1 mRNA expression in the liver and testis and Mt-2 mRNA expression in the liver. Based on hematoxylin-and-eosin staining results, N-ZnO supplementation alleviated histological damage induced by ZnD in the testis and liver. CONCLUSIONS: This study suggested that N-ZnO can be utilized faster than M-ZnO for nutritional restoration at the early stage of ZnD condition and presented Mt-1 as an indicator of Zn status in the serum, liver, and testis.
format Online
Article
Text
id pubmed-9899942
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher The Korean Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-98999422023-02-14 Comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice Yoon, Ja-Seon Nam, Sang Yoon Lee, Beom Jun Lee, Hyun Jik J Vet Sci Original Article BACKGROUND: Zinc (Zn) is an essential cofactor for physiological homeostasis in the body. Zn oxide (ZnO), an inorganic compound that supplies Zn, exists in various sizes, and its bioavailability may vary depending on the size in vivo. However, comparative studies on the nutritional effects of micro-sized ZnO (M-ZnO) and nano-sized ZnO (N-ZnO) supplementation on Zn deficiency (ZnD) animal models have not been reported. OBJECTIVES: This study investigated the nutritional bioavailability of N-ZnO and M-ZnO particles in dietary-induced ZnD mice. METHODS: Animals were divided into six experimental groups: normal group, ZnD control group, and four ZnO treatment groups (Nano-Low, Nano-High, Micro-Low, and Micro-High). After ZnD induction, N-ZnO or M-ZnO was administered orally every day for 4 weeks. RESULTS: ZnD-associated clinical signs almost disappeared 7 days after N-ZnO or M-ZnO administration. Serum Zn concentrations were higher in the Nano-High group than in the ZnD and M-ZnO groups on day 7 of ZnO treatment. In the liver and testis, Nano-Low and Nano-High groups showed significantly higher Zn concentrations than the other groups after 14-day treatment. ZnO supplementation increased Mt-1 mRNA expression in the liver and testis and Mt-2 mRNA expression in the liver. Based on hematoxylin-and-eosin staining results, N-ZnO supplementation alleviated histological damage induced by ZnD in the testis and liver. CONCLUSIONS: This study suggested that N-ZnO can be utilized faster than M-ZnO for nutritional restoration at the early stage of ZnD condition and presented Mt-1 as an indicator of Zn status in the serum, liver, and testis. The Korean Society of Veterinary Science 2022-12-02 /pmc/articles/PMC9899942/ /pubmed/36560835 http://dx.doi.org/10.4142/jvs.22201 Text en © 2023 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yoon, Ja-Seon
Nam, Sang Yoon
Lee, Beom Jun
Lee, Hyun Jik
Comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice
title Comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice
title_full Comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice
title_fullStr Comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice
title_full_unstemmed Comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice
title_short Comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice
title_sort comparative study on the effects of micro- and nano-sized zinc oxide supplementation on zinc-deficient mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899942/
https://www.ncbi.nlm.nih.gov/pubmed/36560835
http://dx.doi.org/10.4142/jvs.22201
work_keys_str_mv AT yoonjaseon comparativestudyontheeffectsofmicroandnanosizedzincoxidesupplementationonzincdeficientmice
AT namsangyoon comparativestudyontheeffectsofmicroandnanosizedzincoxidesupplementationonzincdeficientmice
AT leebeomjun comparativestudyontheeffectsofmicroandnanosizedzincoxidesupplementationonzincdeficientmice
AT leehyunjik comparativestudyontheeffectsofmicroandnanosizedzincoxidesupplementationonzincdeficientmice