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Optimized study of an in vitro 3D culture of preantral follicles in mice
BACKGROUND: In vitro culture of preantral follicles is a promising technology for fertility preservation. OBJECTIVES: This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. METHODS: The is...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899945/ https://www.ncbi.nlm.nih.gov/pubmed/36560836 http://dx.doi.org/10.4142/jvs.22223 |
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author | Ren, Hehe Zhang, Yingxin Zhang, Yanping Qiu, Yikai Chang, Qing Yu, Xiaoli Pei, Xiuying |
author_facet | Ren, Hehe Zhang, Yingxin Zhang, Yanping Qiu, Yikai Chang, Qing Yu, Xiaoli Pei, Xiuying |
author_sort | Ren, Hehe |
collection | PubMed |
description | BACKGROUND: In vitro culture of preantral follicles is a promising technology for fertility preservation. OBJECTIVES: This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. METHODS: The isolated follicles from mouse ovaries were randomly divided in an ultra-low attachment 96-well plates supplement with FBS or bovine serum albumin (BSA) culture or encapsulated with an alginate supplement with FBS or BSA culture. Meanwhile, estradiol (E(2)) concentration was assessed through enzyme-linked immunosorbent assay of culture supernatants. The diameter of follicular growth was measured, and the lumen of the follicle was photographed. Spindle microtubules of oocytes were detected via immunofluorescence. The ability of oocytes to fertilize was assessed using in vitro fertilization. RESULTS: The diameters were larger for the growing secondary follicles cultured in ultra-low attachment 96-well plates than in the alginate gel on days 6, 8, and 10 (p < 0.05). Meanwhile, the E(2) concentration in the BSA-supplemented medium was significantly higher in the alginate gel than in the other three groups on days 6 and 8 (p < 0.05), and the oocytes in the FBS-free system could complete meiosis and fertilization in vitro. CONCLUSIONS: The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system in vitro and supports the clinical practices to achieve competent, mature oocytes for in vitro fertilization. |
format | Online Article Text |
id | pubmed-9899945 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-98999452023-02-14 Optimized study of an in vitro 3D culture of preantral follicles in mice Ren, Hehe Zhang, Yingxin Zhang, Yanping Qiu, Yikai Chang, Qing Yu, Xiaoli Pei, Xiuying J Vet Sci Original Article BACKGROUND: In vitro culture of preantral follicles is a promising technology for fertility preservation. OBJECTIVES: This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. METHODS: The isolated follicles from mouse ovaries were randomly divided in an ultra-low attachment 96-well plates supplement with FBS or bovine serum albumin (BSA) culture or encapsulated with an alginate supplement with FBS or BSA culture. Meanwhile, estradiol (E(2)) concentration was assessed through enzyme-linked immunosorbent assay of culture supernatants. The diameter of follicular growth was measured, and the lumen of the follicle was photographed. Spindle microtubules of oocytes were detected via immunofluorescence. The ability of oocytes to fertilize was assessed using in vitro fertilization. RESULTS: The diameters were larger for the growing secondary follicles cultured in ultra-low attachment 96-well plates than in the alginate gel on days 6, 8, and 10 (p < 0.05). Meanwhile, the E(2) concentration in the BSA-supplemented medium was significantly higher in the alginate gel than in the other three groups on days 6 and 8 (p < 0.05), and the oocytes in the FBS-free system could complete meiosis and fertilization in vitro. CONCLUSIONS: The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system in vitro and supports the clinical practices to achieve competent, mature oocytes for in vitro fertilization. The Korean Society of Veterinary Science 2022-12-05 /pmc/articles/PMC9899945/ /pubmed/36560836 http://dx.doi.org/10.4142/jvs.22223 Text en © 2023 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ren, Hehe Zhang, Yingxin Zhang, Yanping Qiu, Yikai Chang, Qing Yu, Xiaoli Pei, Xiuying Optimized study of an in vitro 3D culture of preantral follicles in mice |
title | Optimized study of an in vitro 3D culture of preantral follicles in mice |
title_full | Optimized study of an in vitro 3D culture of preantral follicles in mice |
title_fullStr | Optimized study of an in vitro 3D culture of preantral follicles in mice |
title_full_unstemmed | Optimized study of an in vitro 3D culture of preantral follicles in mice |
title_short | Optimized study of an in vitro 3D culture of preantral follicles in mice |
title_sort | optimized study of an in vitro 3d culture of preantral follicles in mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9899945/ https://www.ncbi.nlm.nih.gov/pubmed/36560836 http://dx.doi.org/10.4142/jvs.22223 |
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