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Engineered Antiviral Sensor Targets Infected Mosquitoes

Escalating vector disease burdens pose significant global health risks, so innovative tools for targeting mosquitoes are critical. We engineered an antiviral strategy termed REAPER (vRNA Expression Activates Poisonous Effector Ribonuclease) that leverages the programmable RNA-targeting capabilities...

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Autores principales: Benetta, Elena Dalla, López-Denman, Adam J., Li, Hsing-Han, Masri, Reem A., Brogan, Daniel J., Bui, Michelle, Yang, Ting, Li, Ming, Dunn, Michael, Klein, Melissa J., Jackson, Sarah, Catalan, Kyle, Blasdell, Kim R., Tng, Priscilla, Antoshechkin, Igor, Alphey, Luke S., Paradkar, Prasad N., Akbari, Omar S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9900881/
https://www.ncbi.nlm.nih.gov/pubmed/36747634
http://dx.doi.org/10.1101/2023.01.27.525922
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author Benetta, Elena Dalla
López-Denman, Adam J.
Li, Hsing-Han
Masri, Reem A.
Brogan, Daniel J.
Bui, Michelle
Yang, Ting
Li, Ming
Dunn, Michael
Klein, Melissa J.
Jackson, Sarah
Catalan, Kyle
Blasdell, Kim R.
Tng, Priscilla
Antoshechkin, Igor
Alphey, Luke S.
Paradkar, Prasad N.
Akbari, Omar S.
author_facet Benetta, Elena Dalla
López-Denman, Adam J.
Li, Hsing-Han
Masri, Reem A.
Brogan, Daniel J.
Bui, Michelle
Yang, Ting
Li, Ming
Dunn, Michael
Klein, Melissa J.
Jackson, Sarah
Catalan, Kyle
Blasdell, Kim R.
Tng, Priscilla
Antoshechkin, Igor
Alphey, Luke S.
Paradkar, Prasad N.
Akbari, Omar S.
author_sort Benetta, Elena Dalla
collection PubMed
description Escalating vector disease burdens pose significant global health risks, so innovative tools for targeting mosquitoes are critical. We engineered an antiviral strategy termed REAPER (vRNA Expression Activates Poisonous Effector Ribonuclease) that leverages the programmable RNA-targeting capabilities of CRISPR Cas13 and its potent collateral activity. Akin to a stealthy Trojan Horse hiding in stealth awaiting the presence of its enemy, REAPER remains concealed within the mosquito until an infectious blood meal is up taken. Upon target viral RNA infection, REAPER activates, triggering programmed destruction of its target arbovirus such as chikungunya. Consequently, Cas13 mediated RNA targeting significantly reduces viral replication and its promiscuous collateral activity can even kill infected mosquitoes. This innovative REAPER technology adds to an arsenal of effective molecular genetic tools to combat mosquito virus transmission.
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spelling pubmed-99008812023-02-07 Engineered Antiviral Sensor Targets Infected Mosquitoes Benetta, Elena Dalla López-Denman, Adam J. Li, Hsing-Han Masri, Reem A. Brogan, Daniel J. Bui, Michelle Yang, Ting Li, Ming Dunn, Michael Klein, Melissa J. Jackson, Sarah Catalan, Kyle Blasdell, Kim R. Tng, Priscilla Antoshechkin, Igor Alphey, Luke S. Paradkar, Prasad N. Akbari, Omar S. bioRxiv Article Escalating vector disease burdens pose significant global health risks, so innovative tools for targeting mosquitoes are critical. We engineered an antiviral strategy termed REAPER (vRNA Expression Activates Poisonous Effector Ribonuclease) that leverages the programmable RNA-targeting capabilities of CRISPR Cas13 and its potent collateral activity. Akin to a stealthy Trojan Horse hiding in stealth awaiting the presence of its enemy, REAPER remains concealed within the mosquito until an infectious blood meal is up taken. Upon target viral RNA infection, REAPER activates, triggering programmed destruction of its target arbovirus such as chikungunya. Consequently, Cas13 mediated RNA targeting significantly reduces viral replication and its promiscuous collateral activity can even kill infected mosquitoes. This innovative REAPER technology adds to an arsenal of effective molecular genetic tools to combat mosquito virus transmission. Cold Spring Harbor Laboratory 2023-01-27 /pmc/articles/PMC9900881/ /pubmed/36747634 http://dx.doi.org/10.1101/2023.01.27.525922 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Benetta, Elena Dalla
López-Denman, Adam J.
Li, Hsing-Han
Masri, Reem A.
Brogan, Daniel J.
Bui, Michelle
Yang, Ting
Li, Ming
Dunn, Michael
Klein, Melissa J.
Jackson, Sarah
Catalan, Kyle
Blasdell, Kim R.
Tng, Priscilla
Antoshechkin, Igor
Alphey, Luke S.
Paradkar, Prasad N.
Akbari, Omar S.
Engineered Antiviral Sensor Targets Infected Mosquitoes
title Engineered Antiviral Sensor Targets Infected Mosquitoes
title_full Engineered Antiviral Sensor Targets Infected Mosquitoes
title_fullStr Engineered Antiviral Sensor Targets Infected Mosquitoes
title_full_unstemmed Engineered Antiviral Sensor Targets Infected Mosquitoes
title_short Engineered Antiviral Sensor Targets Infected Mosquitoes
title_sort engineered antiviral sensor targets infected mosquitoes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9900881/
https://www.ncbi.nlm.nih.gov/pubmed/36747634
http://dx.doi.org/10.1101/2023.01.27.525922
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