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Environmental and molecular regulation of asexual reproduction in the sea anemone Nematostella vectensis

Cnidarians exhibit incredible reproductive diversity, with most capable of sexual and asexual reproduction. Here we investigate factors that influence asexual reproduction in the burrowing sea anemone Nematostella vectensis, which can propagate asexually by transverse fission of the body column. By...

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Autores principales: Al-Shaer, Layla, Leach, Whitney, Baban, Noor, Yagodich, Mia, Gibson, Mathew C., Layden, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9900923/
https://www.ncbi.nlm.nih.gov/pubmed/36747872
http://dx.doi.org/10.1101/2023.01.27.525773
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author Al-Shaer, Layla
Leach, Whitney
Baban, Noor
Yagodich, Mia
Gibson, Mathew C.
Layden, Michael J.
author_facet Al-Shaer, Layla
Leach, Whitney
Baban, Noor
Yagodich, Mia
Gibson, Mathew C.
Layden, Michael J.
author_sort Al-Shaer, Layla
collection PubMed
description Cnidarians exhibit incredible reproductive diversity, with most capable of sexual and asexual reproduction. Here we investigate factors that influence asexual reproduction in the burrowing sea anemone Nematostella vectensis, which can propagate asexually by transverse fission of the body column. By altering culture conditions, we demonstrate that the presence of a burrowing substrate strongly promotes transverse fission. In addition, we show that animal size does not affect fission rates, and that the plane of fission is fixed along the oral-aboral axis of the polyp. Homeobox transcription factors and components of the TGFβ, Notch, and FGF signaling pathways are differentially expressed in polyps undergoing physal pinching suggesting they are important regulators of transverse fission. Gene ontology analyses further suggest that during transverse fission the cell cycle is suppressed and that cell adhesion and patterning mechanisms are downregulated to promote separation of the body column. Finally, we demonstrate that the rate of asexual reproduction is sensitive to population density. Collectively, these experiments provide a foundation for mechanistic studies of asexual reproduction in Nematostella, with implications for understanding the reproductive and regenerative biology of other cnidarian species.
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spelling pubmed-99009232023-02-07 Environmental and molecular regulation of asexual reproduction in the sea anemone Nematostella vectensis Al-Shaer, Layla Leach, Whitney Baban, Noor Yagodich, Mia Gibson, Mathew C. Layden, Michael J. bioRxiv Article Cnidarians exhibit incredible reproductive diversity, with most capable of sexual and asexual reproduction. Here we investigate factors that influence asexual reproduction in the burrowing sea anemone Nematostella vectensis, which can propagate asexually by transverse fission of the body column. By altering culture conditions, we demonstrate that the presence of a burrowing substrate strongly promotes transverse fission. In addition, we show that animal size does not affect fission rates, and that the plane of fission is fixed along the oral-aboral axis of the polyp. Homeobox transcription factors and components of the TGFβ, Notch, and FGF signaling pathways are differentially expressed in polyps undergoing physal pinching suggesting they are important regulators of transverse fission. Gene ontology analyses further suggest that during transverse fission the cell cycle is suppressed and that cell adhesion and patterning mechanisms are downregulated to promote separation of the body column. Finally, we demonstrate that the rate of asexual reproduction is sensitive to population density. Collectively, these experiments provide a foundation for mechanistic studies of asexual reproduction in Nematostella, with implications for understanding the reproductive and regenerative biology of other cnidarian species. Cold Spring Harbor Laboratory 2023-01-27 /pmc/articles/PMC9900923/ /pubmed/36747872 http://dx.doi.org/10.1101/2023.01.27.525773 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Al-Shaer, Layla
Leach, Whitney
Baban, Noor
Yagodich, Mia
Gibson, Mathew C.
Layden, Michael J.
Environmental and molecular regulation of asexual reproduction in the sea anemone Nematostella vectensis
title Environmental and molecular regulation of asexual reproduction in the sea anemone Nematostella vectensis
title_full Environmental and molecular regulation of asexual reproduction in the sea anemone Nematostella vectensis
title_fullStr Environmental and molecular regulation of asexual reproduction in the sea anemone Nematostella vectensis
title_full_unstemmed Environmental and molecular regulation of asexual reproduction in the sea anemone Nematostella vectensis
title_short Environmental and molecular regulation of asexual reproduction in the sea anemone Nematostella vectensis
title_sort environmental and molecular regulation of asexual reproduction in the sea anemone nematostella vectensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9900923/
https://www.ncbi.nlm.nih.gov/pubmed/36747872
http://dx.doi.org/10.1101/2023.01.27.525773
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