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First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections
Background: prompt recognition and identification of the causative microorganism in acute septic arthritis of native and prosthetic joints is vital to increase the chances of successful treatment. The aim of this study was to independently assess the diagnostic accuracy of the multiplex BIOFIRE(®) J...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Copernicus GmbH
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901515/ https://www.ncbi.nlm.nih.gov/pubmed/36756306 http://dx.doi.org/10.5194/jbji-8-45-2023 |
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author | Schoenmakers, Jorrit Willem Adriaan de Boer, Rosanne Gard, Lilli Kampinga, Greetje Anna van Oosten, Marleen van Dijl, Jan Maarten Jutte, Paulus Christiaan Wouthuyzen-Bakker, Marjan |
author_facet | Schoenmakers, Jorrit Willem Adriaan de Boer, Rosanne Gard, Lilli Kampinga, Greetje Anna van Oosten, Marleen van Dijl, Jan Maarten Jutte, Paulus Christiaan Wouthuyzen-Bakker, Marjan |
author_sort | Schoenmakers, Jorrit Willem Adriaan |
collection | PubMed |
description | Background: prompt recognition and identification of the causative microorganism in acute septic arthritis of native and prosthetic joints is vital to increase the chances of successful treatment. The aim of this study was to independently assess the diagnostic accuracy of the multiplex BIOFIRE(®) Joint Infection (JI) Panel (investigational use only) in synovial fluid for rapid diagnosis. Methods: synovial fluid samples were collected at the University Medical Center Groningen from patients who had a clinical suspicion of a native septic arthritis, early acute (post-operative, within 3 months after arthroplasty) periprosthetic joint infection (PJI) or late acute (hematogenous, [Formula: see text] months after arthroplasty) PJI. JI Panel results were compared to infection according to Musculoskeletal Infection Society criteria and culture-based methods as reference standard. Results: a total of 45 samples were analysed. The BIOFIRE JI Panel showed a high specificity (100 %, 95 % confidence interval (CI): 78–100) in all patient categories. Sensitivity was 83 % (95 % CI: 44–97) for patients with a clinical suspicion of native septic arthritis ( [Formula: see text] ), 73 % (95 % CI: 48–89) for patients with a clinical suspicion of a late acute PJI ( [Formula: see text] ), and 30 % (95 % CI: 11–60) for patients with a clinical suspicion of an early acute PJI ( [Formula: see text] ). Conclusion: the results of this study indicate a clear clinical benefit of the BIOFIRE JI Panel in patients with a suspected native septic arthritis and late acute (hematogenous) PJI, but a low clinical benefit in patients with an early acute (post-operative) PJI due to the absence of certain relevant microorganisms, such as Staphylococcus epidermidis, from the panel. |
format | Online Article Text |
id | pubmed-9901515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Copernicus GmbH |
record_format | MEDLINE/PubMed |
spelling | pubmed-99015152023-02-07 First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections Schoenmakers, Jorrit Willem Adriaan de Boer, Rosanne Gard, Lilli Kampinga, Greetje Anna van Oosten, Marleen van Dijl, Jan Maarten Jutte, Paulus Christiaan Wouthuyzen-Bakker, Marjan J Bone Jt Infect Original Full-Length Article Background: prompt recognition and identification of the causative microorganism in acute septic arthritis of native and prosthetic joints is vital to increase the chances of successful treatment. The aim of this study was to independently assess the diagnostic accuracy of the multiplex BIOFIRE(®) Joint Infection (JI) Panel (investigational use only) in synovial fluid for rapid diagnosis. Methods: synovial fluid samples were collected at the University Medical Center Groningen from patients who had a clinical suspicion of a native septic arthritis, early acute (post-operative, within 3 months after arthroplasty) periprosthetic joint infection (PJI) or late acute (hematogenous, [Formula: see text] months after arthroplasty) PJI. JI Panel results were compared to infection according to Musculoskeletal Infection Society criteria and culture-based methods as reference standard. Results: a total of 45 samples were analysed. The BIOFIRE JI Panel showed a high specificity (100 %, 95 % confidence interval (CI): 78–100) in all patient categories. Sensitivity was 83 % (95 % CI: 44–97) for patients with a clinical suspicion of native septic arthritis ( [Formula: see text] ), 73 % (95 % CI: 48–89) for patients with a clinical suspicion of a late acute PJI ( [Formula: see text] ), and 30 % (95 % CI: 11–60) for patients with a clinical suspicion of an early acute PJI ( [Formula: see text] ). Conclusion: the results of this study indicate a clear clinical benefit of the BIOFIRE JI Panel in patients with a suspected native septic arthritis and late acute (hematogenous) PJI, but a low clinical benefit in patients with an early acute (post-operative) PJI due to the absence of certain relevant microorganisms, such as Staphylococcus epidermidis, from the panel. Copernicus GmbH 2023-01-18 /pmc/articles/PMC9901515/ /pubmed/36756306 http://dx.doi.org/10.5194/jbji-8-45-2023 Text en Copyright: © 2023 Jorrit Willem Adriaan Schoenmakers et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Original Full-Length Article Schoenmakers, Jorrit Willem Adriaan de Boer, Rosanne Gard, Lilli Kampinga, Greetje Anna van Oosten, Marleen van Dijl, Jan Maarten Jutte, Paulus Christiaan Wouthuyzen-Bakker, Marjan First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections |
title | First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections |
title_full | First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections |
title_fullStr | First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections |
title_full_unstemmed | First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections |
title_short | First evaluation of a commercial multiplex PCR panel for rapid detection of pathogens associated with acute joint infections |
title_sort | first evaluation of a commercial multiplex pcr panel for rapid detection of pathogens associated with acute joint infections |
topic | Original Full-Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901515/ https://www.ncbi.nlm.nih.gov/pubmed/36756306 http://dx.doi.org/10.5194/jbji-8-45-2023 |
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