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Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of children and adolescents infected with HIV and uninfected controls
Immune exhaustion and senescence are scarcely studied in HIV-pediatric patients. We studied the circulatory CD8 T cells activation/exhaustion and senescent phenotype of children and adolescents vertically infected with HIV or uninfected controls based on the expression of human leukocyte antigen (HL...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto de Medicina Tropical
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901578/ https://www.ncbi.nlm.nih.gov/pubmed/36753067 http://dx.doi.org/10.1590/S1678-9946202365014 |
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author | Pereira-Manfro, Wânia Ferraz da Silva, Giselle Pereira Costa, Priscilla Ramos Costa, Dayane Alves Ferreira, Bianca da Silva Barreto, Daniela Mena Frota, Ana Cristina Cisne Hofer, Cristina Barroso Kallas, Esper Georges Milagres, Lucimar Gonçalves |
author_facet | Pereira-Manfro, Wânia Ferraz da Silva, Giselle Pereira Costa, Priscilla Ramos Costa, Dayane Alves Ferreira, Bianca da Silva Barreto, Daniela Mena Frota, Ana Cristina Cisne Hofer, Cristina Barroso Kallas, Esper Georges Milagres, Lucimar Gonçalves |
author_sort | Pereira-Manfro, Wânia Ferraz |
collection | PubMed |
description | Immune exhaustion and senescence are scarcely studied in HIV-pediatric patients. We studied the circulatory CD8 T cells activation/exhaustion and senescent phenotype of children and adolescents vertically infected with HIV or uninfected controls based on the expression of human leukocyte antigen (HLA-DR), CD38, T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain (TIGIT), programmed death 1 (PD-1) and CD57 by flow cytometry, during approximately one year. Eleven HIV-infected (HI) and nine HIV-uninfected (HU) children/adolescents who received two doses or one dose of meningococcal C conjugate vaccine (MenC), respectively, were involved in this study. Blood samples were collected before the immunization (T0), 1–2 months after the first dose (T1), and 1–2 months after the second dose (T2), which was administered approximately one year after the first one. HI patients not receiving combined antiretroviral therapy (cART) showed a higher frequency of CD8 T cells TIGIT(+), PD-1(+) or CD57(+), as well as a higher frequency of CD8 T cells co-expressing CD38/HLA-DR/TIGIT or CD38/HLA-DR/PD-1 when compared to HI treated or HU individuals, at all times that they were assessed. CD8 T cells co-expressing CD38/DR/TIGIT were inversely correlated with the CD4/CD8 ratio but positively associated with viral load. The co-expression of CD38/DR/TIGIT or CD38/DR/PD-1 on CD8 T cells was also inversely associated with the CD4 T cells expressing co-stimulatory molecules CD127/CD28. The results showed a higher expression of exhaustion/senescence markers on CD8 T cells of untreated HI children/adolescents and its correlations with viral load. |
format | Online Article Text |
id | pubmed-9901578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Instituto de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-99015782023-02-07 Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of children and adolescents infected with HIV and uninfected controls Pereira-Manfro, Wânia Ferraz da Silva, Giselle Pereira Costa, Priscilla Ramos Costa, Dayane Alves Ferreira, Bianca da Silva Barreto, Daniela Mena Frota, Ana Cristina Cisne Hofer, Cristina Barroso Kallas, Esper Georges Milagres, Lucimar Gonçalves Rev Inst Med Trop Sao Paulo Brief Communication Immune exhaustion and senescence are scarcely studied in HIV-pediatric patients. We studied the circulatory CD8 T cells activation/exhaustion and senescent phenotype of children and adolescents vertically infected with HIV or uninfected controls based on the expression of human leukocyte antigen (HLA-DR), CD38, T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain (TIGIT), programmed death 1 (PD-1) and CD57 by flow cytometry, during approximately one year. Eleven HIV-infected (HI) and nine HIV-uninfected (HU) children/adolescents who received two doses or one dose of meningococcal C conjugate vaccine (MenC), respectively, were involved in this study. Blood samples were collected before the immunization (T0), 1–2 months after the first dose (T1), and 1–2 months after the second dose (T2), which was administered approximately one year after the first one. HI patients not receiving combined antiretroviral therapy (cART) showed a higher frequency of CD8 T cells TIGIT(+), PD-1(+) or CD57(+), as well as a higher frequency of CD8 T cells co-expressing CD38/HLA-DR/TIGIT or CD38/HLA-DR/PD-1 when compared to HI treated or HU individuals, at all times that they were assessed. CD8 T cells co-expressing CD38/DR/TIGIT were inversely correlated with the CD4/CD8 ratio but positively associated with viral load. The co-expression of CD38/DR/TIGIT or CD38/DR/PD-1 on CD8 T cells was also inversely associated with the CD4 T cells expressing co-stimulatory molecules CD127/CD28. The results showed a higher expression of exhaustion/senescence markers on CD8 T cells of untreated HI children/adolescents and its correlations with viral load. Instituto de Medicina Tropical 2023-02-06 /pmc/articles/PMC9901578/ /pubmed/36753067 http://dx.doi.org/10.1590/S1678-9946202365014 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Communication Pereira-Manfro, Wânia Ferraz da Silva, Giselle Pereira Costa, Priscilla Ramos Costa, Dayane Alves Ferreira, Bianca da Silva Barreto, Daniela Mena Frota, Ana Cristina Cisne Hofer, Cristina Barroso Kallas, Esper Georges Milagres, Lucimar Gonçalves Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of children and adolescents infected with HIV and uninfected controls |
title | Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of
children and adolescents infected with HIV and uninfected
controls |
title_full | Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of
children and adolescents infected with HIV and uninfected
controls |
title_fullStr | Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of
children and adolescents infected with HIV and uninfected
controls |
title_full_unstemmed | Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of
children and adolescents infected with HIV and uninfected
controls |
title_short | Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of
children and adolescents infected with HIV and uninfected
controls |
title_sort | expression of tigit, pd-1 and hla-dr/cd38 markers on cd8-t cells of
children and adolescents infected with hiv and uninfected
controls |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901578/ https://www.ncbi.nlm.nih.gov/pubmed/36753067 http://dx.doi.org/10.1590/S1678-9946202365014 |
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