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Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil

Austrodiplostomum spp. (Platyhelminthes: Digenea) are endoparasites with a broad geographic distribution in South America. During the larval stage, they parasitize the eyes, brains, muscles, gill, kidneys and swim bladder of a wide variety of fishes. The metacercariae of Austrodiplostomum spp. have...

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Autores principales: Cracco, Aparecida de Fátima, Scorsim, Bárbara, de Oliveira, Alessandra Valéria, Takemoto, Ricardo Massato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Parasitologia Veterinária 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901856/
https://www.ncbi.nlm.nih.gov/pubmed/35476009
http://dx.doi.org/10.1590/S1984-29612022021
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author Cracco, Aparecida de Fátima
Scorsim, Bárbara
de Oliveira, Alessandra Valéria
Takemoto, Ricardo Massato
author_facet Cracco, Aparecida de Fátima
Scorsim, Bárbara
de Oliveira, Alessandra Valéria
Takemoto, Ricardo Massato
author_sort Cracco, Aparecida de Fátima
collection PubMed
description Austrodiplostomum spp. (Platyhelminthes: Digenea) are endoparasites with a broad geographic distribution in South America. During the larval stage, they parasitize the eyes, brains, muscles, gill, kidneys and swim bladder of a wide variety of fishes. The metacercariae of Austrodiplostomum spp. have several morphological characteristics during development, but are very similar among species, which makes it necessary to use molecular tools to contribute to the elucidation during the larval stage. The objective of this study was to perform morphological and molecular analyses of Austrodiplostomum sp. found in specimens of Hypostomus sourced from the Ivaí River in the state of Paraná, Brazil. Of the 93 analyzed specimens (H. hermanni [n = 50], H. albopunctatus [n = 9], Hypostomus sp. 1 [n = 24], and Hypostomus sp. 2 [n = 10]), 60 were parasitized. A total of 577 Austrodiplostomum sp. metacercariae was collected from the infected hosts; DNA from seven of these samples was extracted, amplified, and sequenced. The morphological data associated with the genetic distance values and the relationships observed in the COI gene tree, indicate that all metacercariae were A. compactum. This is the first record of A. compactum parasitizing H. hermanni, H. albopunctatus, Hypostomus sp. 1, and Hypostomus sp. 2 in the Ivaí River.
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spelling pubmed-99018562023-03-09 Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil Cracco, Aparecida de Fátima Scorsim, Bárbara de Oliveira, Alessandra Valéria Takemoto, Ricardo Massato Rev Bras Parasitol Vet Original Article Austrodiplostomum spp. (Platyhelminthes: Digenea) are endoparasites with a broad geographic distribution in South America. During the larval stage, they parasitize the eyes, brains, muscles, gill, kidneys and swim bladder of a wide variety of fishes. The metacercariae of Austrodiplostomum spp. have several morphological characteristics during development, but are very similar among species, which makes it necessary to use molecular tools to contribute to the elucidation during the larval stage. The objective of this study was to perform morphological and molecular analyses of Austrodiplostomum sp. found in specimens of Hypostomus sourced from the Ivaí River in the state of Paraná, Brazil. Of the 93 analyzed specimens (H. hermanni [n = 50], H. albopunctatus [n = 9], Hypostomus sp. 1 [n = 24], and Hypostomus sp. 2 [n = 10]), 60 were parasitized. A total of 577 Austrodiplostomum sp. metacercariae was collected from the infected hosts; DNA from seven of these samples was extracted, amplified, and sequenced. The morphological data associated with the genetic distance values and the relationships observed in the COI gene tree, indicate that all metacercariae were A. compactum. This is the first record of A. compactum parasitizing H. hermanni, H. albopunctatus, Hypostomus sp. 1, and Hypostomus sp. 2 in the Ivaí River. Colégio Brasileiro de Parasitologia Veterinária 2022-04-22 /pmc/articles/PMC9901856/ /pubmed/35476009 http://dx.doi.org/10.1590/S1984-29612022021 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Cracco, Aparecida de Fátima
Scorsim, Bárbara
de Oliveira, Alessandra Valéria
Takemoto, Ricardo Massato
Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil
title Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil
title_full Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil
title_fullStr Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil
title_full_unstemmed Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil
title_short Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil
title_sort morphological and molecular characterization of austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the ivaí river, brazil
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901856/
https://www.ncbi.nlm.nih.gov/pubmed/35476009
http://dx.doi.org/10.1590/S1984-29612022021
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