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Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil
Austrodiplostomum spp. (Platyhelminthes: Digenea) are endoparasites with a broad geographic distribution in South America. During the larval stage, they parasitize the eyes, brains, muscles, gill, kidneys and swim bladder of a wide variety of fishes. The metacercariae of Austrodiplostomum spp. have...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Colégio Brasileiro de Parasitologia Veterinária
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901856/ https://www.ncbi.nlm.nih.gov/pubmed/35476009 http://dx.doi.org/10.1590/S1984-29612022021 |
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author | Cracco, Aparecida de Fátima Scorsim, Bárbara de Oliveira, Alessandra Valéria Takemoto, Ricardo Massato |
author_facet | Cracco, Aparecida de Fátima Scorsim, Bárbara de Oliveira, Alessandra Valéria Takemoto, Ricardo Massato |
author_sort | Cracco, Aparecida de Fátima |
collection | PubMed |
description | Austrodiplostomum spp. (Platyhelminthes: Digenea) are endoparasites with a broad geographic distribution in South America. During the larval stage, they parasitize the eyes, brains, muscles, gill, kidneys and swim bladder of a wide variety of fishes. The metacercariae of Austrodiplostomum spp. have several morphological characteristics during development, but are very similar among species, which makes it necessary to use molecular tools to contribute to the elucidation during the larval stage. The objective of this study was to perform morphological and molecular analyses of Austrodiplostomum sp. found in specimens of Hypostomus sourced from the Ivaí River in the state of Paraná, Brazil. Of the 93 analyzed specimens (H. hermanni [n = 50], H. albopunctatus [n = 9], Hypostomus sp. 1 [n = 24], and Hypostomus sp. 2 [n = 10]), 60 were parasitized. A total of 577 Austrodiplostomum sp. metacercariae was collected from the infected hosts; DNA from seven of these samples was extracted, amplified, and sequenced. The morphological data associated with the genetic distance values and the relationships observed in the COI gene tree, indicate that all metacercariae were A. compactum. This is the first record of A. compactum parasitizing H. hermanni, H. albopunctatus, Hypostomus sp. 1, and Hypostomus sp. 2 in the Ivaí River. |
format | Online Article Text |
id | pubmed-9901856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Colégio Brasileiro de Parasitologia Veterinária |
record_format | MEDLINE/PubMed |
spelling | pubmed-99018562023-03-09 Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil Cracco, Aparecida de Fátima Scorsim, Bárbara de Oliveira, Alessandra Valéria Takemoto, Ricardo Massato Rev Bras Parasitol Vet Original Article Austrodiplostomum spp. (Platyhelminthes: Digenea) are endoparasites with a broad geographic distribution in South America. During the larval stage, they parasitize the eyes, brains, muscles, gill, kidneys and swim bladder of a wide variety of fishes. The metacercariae of Austrodiplostomum spp. have several morphological characteristics during development, but are very similar among species, which makes it necessary to use molecular tools to contribute to the elucidation during the larval stage. The objective of this study was to perform morphological and molecular analyses of Austrodiplostomum sp. found in specimens of Hypostomus sourced from the Ivaí River in the state of Paraná, Brazil. Of the 93 analyzed specimens (H. hermanni [n = 50], H. albopunctatus [n = 9], Hypostomus sp. 1 [n = 24], and Hypostomus sp. 2 [n = 10]), 60 were parasitized. A total of 577 Austrodiplostomum sp. metacercariae was collected from the infected hosts; DNA from seven of these samples was extracted, amplified, and sequenced. The morphological data associated with the genetic distance values and the relationships observed in the COI gene tree, indicate that all metacercariae were A. compactum. This is the first record of A. compactum parasitizing H. hermanni, H. albopunctatus, Hypostomus sp. 1, and Hypostomus sp. 2 in the Ivaí River. Colégio Brasileiro de Parasitologia Veterinária 2022-04-22 /pmc/articles/PMC9901856/ /pubmed/35476009 http://dx.doi.org/10.1590/S1984-29612022021 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cracco, Aparecida de Fátima Scorsim, Bárbara de Oliveira, Alessandra Valéria Takemoto, Ricardo Massato Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil |
title | Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil |
title_full | Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil |
title_fullStr | Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil |
title_full_unstemmed | Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil |
title_short | Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil |
title_sort | morphological and molecular characterization of austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the ivaí river, brazil |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901856/ https://www.ncbi.nlm.nih.gov/pubmed/35476009 http://dx.doi.org/10.1590/S1984-29612022021 |
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