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Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples
The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds. A total of 443 genomic DNA extracted from avian fecal samples were analyzed by one-tube nested real-time PCR and conventio...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Parasitologia Veterinária
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901886/ https://www.ncbi.nlm.nih.gov/pubmed/35320826 http://dx.doi.org/10.1590/S1984-29612022017 |