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Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples

The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds. A total of 443 genomic DNA extracted from avian fecal samples were analyzed by one-tube nested real-time PCR and conventio...

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Detalles Bibliográficos
Autores principales: Santana, Bruna Nicoleti, Ferrari, Elis Domingos, Nakamura, Alex Akira, da Silva, Giane Serafim, Meireles, Marcelo Vasconcelos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Parasitologia Veterinária 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901886/
https://www.ncbi.nlm.nih.gov/pubmed/35320826
http://dx.doi.org/10.1590/S1984-29612022017