Cargando…
Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage
In order to investigate the effects of dietary probiotics supplementation on laying performance, egg quality, serum hormone levels, immunity, antioxidant, and gut microbiota of layers at different laying stages, a total of 168 Tianfu green shell laying hens (28-day-old) were randomly divided into 2...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9902371/ https://www.ncbi.nlm.nih.gov/pubmed/36760506 http://dx.doi.org/10.3389/fmicb.2023.1041072 |
_version_ | 1784883246159364096 |
---|---|
author | Xu, Hengyong Lu, Yuxiang Li, Dan Yan, Chaoyang Jiang, Yuru Hu, Zhi Zhang, Zhipeng Du, Ranran Zhao, Xiaoling Zhang, Yao Tian, Yaofu Zhu, Qing Liu, Yiping Wang, Yan |
author_facet | Xu, Hengyong Lu, Yuxiang Li, Dan Yan, Chaoyang Jiang, Yuru Hu, Zhi Zhang, Zhipeng Du, Ranran Zhao, Xiaoling Zhang, Yao Tian, Yaofu Zhu, Qing Liu, Yiping Wang, Yan |
author_sort | Xu, Hengyong |
collection | PubMed |
description | In order to investigate the effects of dietary probiotics supplementation on laying performance, egg quality, serum hormone levels, immunity, antioxidant, and gut microbiota of layers at different laying stages, a total of 168 Tianfu green shell laying hens (28-day-old) were randomly divided into 2 treatments: a non-supplemented control diet (NC), and diet supplemented with 10 g/kg of probiotics, respectively. Each treatment had 6 replicates with 14 hens per replicate. The feeding trial lasted for 54 weeks. The results showed that the supplementation of probiotics significantly increased the average egg weight, improved egg quality (p < 0.05) and ovarian development. Meanwhile, probiotics increased the serum hormone levels of E(2) and FSH, and antioxidant indices T-AOC and T-SOD (p < 0.05) of laying hens at different laying stages (p < 0.05), decreased the expression of proinflammatory factors including IL-1, IL-6 and TNF-α (p < 0.05). Furthermore, using 16S rRNA sequencing, we observed that the addition of probiotics increased the distribution of Firmicutes, Bacteroidota and Synergistota at early laying period. Meanwhile, Bacteroidota, Actinobacteriota, Verrucomicrobiota and Deferribacterota showed an increasing trend at the peak of egg production. The relative abundance of Firmicutes, Desulfobacterota and Actinobacteriota were significantly increased at the late laying period. Moreover, PICRUSt2 and BugBase analysis revealed that at the late laying period, the probiotics supplementation not only enriched many significant gene clusters of the metabolism of terpenoids and polyketide, genetic information processing, enzyme families, translation, transcription, replication and repair, and nucleotide metabolism, but also decreased the proportion of potential pathogenic bacteria. To sum up, these data show that the addition of probiotics not only improves the performance, egg quality, ovarian development and immune function of laying hens at different laying period, but also improves the gut microbiota of layers, thus enhances production efficiency. |
format | Online Article Text |
id | pubmed-9902371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99023712023-02-08 Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage Xu, Hengyong Lu, Yuxiang Li, Dan Yan, Chaoyang Jiang, Yuru Hu, Zhi Zhang, Zhipeng Du, Ranran Zhao, Xiaoling Zhang, Yao Tian, Yaofu Zhu, Qing Liu, Yiping Wang, Yan Front Microbiol Microbiology In order to investigate the effects of dietary probiotics supplementation on laying performance, egg quality, serum hormone levels, immunity, antioxidant, and gut microbiota of layers at different laying stages, a total of 168 Tianfu green shell laying hens (28-day-old) were randomly divided into 2 treatments: a non-supplemented control diet (NC), and diet supplemented with 10 g/kg of probiotics, respectively. Each treatment had 6 replicates with 14 hens per replicate. The feeding trial lasted for 54 weeks. The results showed that the supplementation of probiotics significantly increased the average egg weight, improved egg quality (p < 0.05) and ovarian development. Meanwhile, probiotics increased the serum hormone levels of E(2) and FSH, and antioxidant indices T-AOC and T-SOD (p < 0.05) of laying hens at different laying stages (p < 0.05), decreased the expression of proinflammatory factors including IL-1, IL-6 and TNF-α (p < 0.05). Furthermore, using 16S rRNA sequencing, we observed that the addition of probiotics increased the distribution of Firmicutes, Bacteroidota and Synergistota at early laying period. Meanwhile, Bacteroidota, Actinobacteriota, Verrucomicrobiota and Deferribacterota showed an increasing trend at the peak of egg production. The relative abundance of Firmicutes, Desulfobacterota and Actinobacteriota were significantly increased at the late laying period. Moreover, PICRUSt2 and BugBase analysis revealed that at the late laying period, the probiotics supplementation not only enriched many significant gene clusters of the metabolism of terpenoids and polyketide, genetic information processing, enzyme families, translation, transcription, replication and repair, and nucleotide metabolism, but also decreased the proportion of potential pathogenic bacteria. To sum up, these data show that the addition of probiotics not only improves the performance, egg quality, ovarian development and immune function of laying hens at different laying period, but also improves the gut microbiota of layers, thus enhances production efficiency. Frontiers Media S.A. 2023-01-24 /pmc/articles/PMC9902371/ /pubmed/36760506 http://dx.doi.org/10.3389/fmicb.2023.1041072 Text en Copyright © 2023 Xu, Lu, Li, Yan, Jiang, Hu, Zhang, Du, Zhao, Zhang, Tian, Zhu, Liu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Xu, Hengyong Lu, Yuxiang Li, Dan Yan, Chaoyang Jiang, Yuru Hu, Zhi Zhang, Zhipeng Du, Ranran Zhao, Xiaoling Zhang, Yao Tian, Yaofu Zhu, Qing Liu, Yiping Wang, Yan Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage |
title | Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage |
title_full | Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage |
title_fullStr | Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage |
title_full_unstemmed | Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage |
title_short | Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage |
title_sort | probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9902371/ https://www.ncbi.nlm.nih.gov/pubmed/36760506 http://dx.doi.org/10.3389/fmicb.2023.1041072 |
work_keys_str_mv | AT xuhengyong probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT luyuxiang probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT lidan probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT yanchaoyang probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT jiangyuru probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT huzhi probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT zhangzhipeng probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT duranran probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT zhaoxiaoling probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT zhangyao probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT tianyaofu probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT zhuqing probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT liuyiping probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage AT wangyan probioticmediatedintestinalmicrobiotaandimprovedperformanceeggqualityandovarianimmunefunctionoflayinghensatdifferentlayingstage |