Ocean warming and acidification affect the transitional C:N:P ratio and macromolecular accumulation in the harmful raphidophyte Heterosigma akashiwo

Despite an increase in ocean warming and acidification that is expected to increase the number of harmful algal species worldwide, the population of the raphidophyte Heterosigma akashiwo has been reported to be reduced. However, how this species physically and metabolically modifies transitional C:N:P ratio and macromolecule accumulation is unknown. Considering 1st, 10th, and 20th culture generations under present (low-temperature; low-carbon-dioxide [LTLC] 21 °C; pCO(2) 400 ppm) and future (high-temperature; high-carbon-dioxide [HTHC] 25 °C; pCO(2) 1000 ppm) ocean conditions, we examined transitional C:N:P ratio and macromolecule level changes and performed transcriptome sequencing. The results showed that compared to 1st generation cells, 20th generation cells under HTHC conditions showed a large decrease in carbon quota (Q(C): 34%), nitrogen quota (Q(N): 36%), and phosphorus quota (Q(P): 32%), which were reflected in an overall reduction in DNA and RNA quantity. Decreased activation of photosynthetic, carbon fixation and lipid metabolic pathways coincided with changes in photosynthetic efficiency, carbon concentration, and lipid accumulation after long-term (20th generation) exposure to HTHC conditions. We observed that these variations in internal metabolic pathways were caused by external changes in temperature, which activated the (Ca(+)) signaling pathway, and external changes in pCO(2), which altered proton exchange pathways. Our results suggest that H. akashiwo in a temperate environment will undergo profound changes in C:N:P ratio and macromolecular properties, leading to programmed cell death, in the future.