Cargando…

Potential roles of acyl homoserine lactones (AHLs) in nitrifying bacteria survival under certain adverse circumstances

Potential roles of quorum sensing (QS) in nitrifying bacteria activity and ecology, particularly under adverse circumstances have been rarely reported. Herein, eight lab-scale nitrification sequencing batch reactors, with or without adding acyl homoserine lactones (AHLs) were operated under adverse...

Descripción completa

Detalles Bibliográficos
Autores principales: Zeng, Xiangguo, Hu, Huizhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9902454/
https://www.ncbi.nlm.nih.gov/pubmed/36747059
http://dx.doi.org/10.1038/s41598-022-23123-x
_version_ 1784883265292730368
author Zeng, Xiangguo
Hu, Huizhi
author_facet Zeng, Xiangguo
Hu, Huizhi
author_sort Zeng, Xiangguo
collection PubMed
description Potential roles of quorum sensing (QS) in nitrifying bacteria activity and ecology, particularly under adverse circumstances have been rarely reported. Herein, eight lab-scale nitrification sequencing batch reactors, with or without adding acyl homoserine lactones (AHLs) were operated under adverse circumstances respectively. The results indicated that the introduction of AHLs significantly enhanced the nitrogen removal efficiency in the presence of nitrification inhibitors (dicyandiamide, DCD), accelerated the low temperature (10 °C) group into stable stage, and improved the utilization efficiency of AHLs in these two groups. Community analysis and qPCR further confirmed that AHLs significantly increased the abundance of nitrifying bacteria in low temperature group and DCD group, especially AOB. For normal condition (28 °C, pH = 8) or low pH level (5.5), however, the AHLs had no significant effect. Canonical correspondence analysis showed that nitrifying bacteria positively responded to AHLs, indicating that adding AHLs was an effective strategy to regulate nitrification process. However, under acid conditions, the effect of this regulatory mechanism was not significant, indicating that the influence of pH on the system was greater than that of AHLs. This study demonstrated that exogenous AHLs could enhance the competitiveness of nitrifying bacteria to utilize more resource and occupy space under some adverse environmental conditions.
format Online
Article
Text
id pubmed-9902454
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-99024542023-02-08 Potential roles of acyl homoserine lactones (AHLs) in nitrifying bacteria survival under certain adverse circumstances Zeng, Xiangguo Hu, Huizhi Sci Rep Article Potential roles of quorum sensing (QS) in nitrifying bacteria activity and ecology, particularly under adverse circumstances have been rarely reported. Herein, eight lab-scale nitrification sequencing batch reactors, with or without adding acyl homoserine lactones (AHLs) were operated under adverse circumstances respectively. The results indicated that the introduction of AHLs significantly enhanced the nitrogen removal efficiency in the presence of nitrification inhibitors (dicyandiamide, DCD), accelerated the low temperature (10 °C) group into stable stage, and improved the utilization efficiency of AHLs in these two groups. Community analysis and qPCR further confirmed that AHLs significantly increased the abundance of nitrifying bacteria in low temperature group and DCD group, especially AOB. For normal condition (28 °C, pH = 8) or low pH level (5.5), however, the AHLs had no significant effect. Canonical correspondence analysis showed that nitrifying bacteria positively responded to AHLs, indicating that adding AHLs was an effective strategy to regulate nitrification process. However, under acid conditions, the effect of this regulatory mechanism was not significant, indicating that the influence of pH on the system was greater than that of AHLs. This study demonstrated that exogenous AHLs could enhance the competitiveness of nitrifying bacteria to utilize more resource and occupy space under some adverse environmental conditions. Nature Publishing Group UK 2023-02-06 /pmc/articles/PMC9902454/ /pubmed/36747059 http://dx.doi.org/10.1038/s41598-022-23123-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zeng, Xiangguo
Hu, Huizhi
Potential roles of acyl homoserine lactones (AHLs) in nitrifying bacteria survival under certain adverse circumstances
title Potential roles of acyl homoserine lactones (AHLs) in nitrifying bacteria survival under certain adverse circumstances
title_full Potential roles of acyl homoserine lactones (AHLs) in nitrifying bacteria survival under certain adverse circumstances
title_fullStr Potential roles of acyl homoserine lactones (AHLs) in nitrifying bacteria survival under certain adverse circumstances
title_full_unstemmed Potential roles of acyl homoserine lactones (AHLs) in nitrifying bacteria survival under certain adverse circumstances
title_short Potential roles of acyl homoserine lactones (AHLs) in nitrifying bacteria survival under certain adverse circumstances
title_sort potential roles of acyl homoserine lactones (ahls) in nitrifying bacteria survival under certain adverse circumstances
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9902454/
https://www.ncbi.nlm.nih.gov/pubmed/36747059
http://dx.doi.org/10.1038/s41598-022-23123-x
work_keys_str_mv AT zengxiangguo potentialrolesofacylhomoserinelactonesahlsinnitrifyingbacteriasurvivalundercertainadversecircumstances
AT huhuizhi potentialrolesofacylhomoserinelactonesahlsinnitrifyingbacteriasurvivalundercertainadversecircumstances