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A Novel Three-Dimensional Approach Towards Evaluating Endomyocardial Biopsies for Follow-Up After Heart Transplantation: X-Ray Phase Contrast Imaging and Its Agreement With Classical Histopathology

Endomyocardial biopsies are the gold standard for surveillance of graft rejection following heart transplantation, and are assessed by classical histopathology using a limited number of previously stained slices from several biopsies. Synchrotron propagation-based X-ray phase contrast imaging is a n...

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Autores principales: Planinc, Ivo, Ilic, Ivana, Dejea, Hector, Garcia-Canadilla, Patricia, Gasparovic, Hrvoje, Jurin, Hrvoje, Milicic, Davor, Skoric, Bosko, Stampanoni, Marco, Bijnens, Bart, Bonnin, Anne, Cikes, Maja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9904361/
https://www.ncbi.nlm.nih.gov/pubmed/36762268
http://dx.doi.org/10.3389/ti.2023.11046
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author Planinc, Ivo
Ilic, Ivana
Dejea, Hector
Garcia-Canadilla, Patricia
Gasparovic, Hrvoje
Jurin, Hrvoje
Milicic, Davor
Skoric, Bosko
Stampanoni, Marco
Bijnens, Bart
Bonnin, Anne
Cikes, Maja
author_facet Planinc, Ivo
Ilic, Ivana
Dejea, Hector
Garcia-Canadilla, Patricia
Gasparovic, Hrvoje
Jurin, Hrvoje
Milicic, Davor
Skoric, Bosko
Stampanoni, Marco
Bijnens, Bart
Bonnin, Anne
Cikes, Maja
author_sort Planinc, Ivo
collection PubMed
description Endomyocardial biopsies are the gold standard for surveillance of graft rejection following heart transplantation, and are assessed by classical histopathology using a limited number of previously stained slices from several biopsies. Synchrotron propagation-based X-ray phase contrast imaging is a non-destructive method to image biological samples without tissue preparation, enabling virtual 2D and 3D histopathology. We aimed to show the feasibility of this method to assess acute cellular rejection and its agreement to classical histopathology. Right ventricular biopsies were sampled from 23 heart transplantation recipients (20 males, mean age 54±14 years) as part of standard follow-up. The clinical diagnosis of potential rejection was made using classical histopathology. One additional study sample was harvested and imaged by X-ray phase contrast imaging, producing 3D datasets with 0.65 μm pixel size, and up to 4,320 images per sample. An experienced pathologist graded both histopathological and X-ray phase contrast images in a blinded fashion. The agreement between methods was assessed by weighted kappa, showing substantial agreement (kappa up to 0.80, p < 0.01) between X-ray phase contrast imaging and classical histopathology. X-ray phase contrast imaging does not require tissue processing, allows thorough analysis of a full myocardial sample and allows identification of acute cellular rejection.
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spelling pubmed-99043612023-02-08 A Novel Three-Dimensional Approach Towards Evaluating Endomyocardial Biopsies for Follow-Up After Heart Transplantation: X-Ray Phase Contrast Imaging and Its Agreement With Classical Histopathology Planinc, Ivo Ilic, Ivana Dejea, Hector Garcia-Canadilla, Patricia Gasparovic, Hrvoje Jurin, Hrvoje Milicic, Davor Skoric, Bosko Stampanoni, Marco Bijnens, Bart Bonnin, Anne Cikes, Maja Transpl Int Health Archive Endomyocardial biopsies are the gold standard for surveillance of graft rejection following heart transplantation, and are assessed by classical histopathology using a limited number of previously stained slices from several biopsies. Synchrotron propagation-based X-ray phase contrast imaging is a non-destructive method to image biological samples without tissue preparation, enabling virtual 2D and 3D histopathology. We aimed to show the feasibility of this method to assess acute cellular rejection and its agreement to classical histopathology. Right ventricular biopsies were sampled from 23 heart transplantation recipients (20 males, mean age 54±14 years) as part of standard follow-up. The clinical diagnosis of potential rejection was made using classical histopathology. One additional study sample was harvested and imaged by X-ray phase contrast imaging, producing 3D datasets with 0.65 μm pixel size, and up to 4,320 images per sample. An experienced pathologist graded both histopathological and X-ray phase contrast images in a blinded fashion. The agreement between methods was assessed by weighted kappa, showing substantial agreement (kappa up to 0.80, p < 0.01) between X-ray phase contrast imaging and classical histopathology. X-ray phase contrast imaging does not require tissue processing, allows thorough analysis of a full myocardial sample and allows identification of acute cellular rejection. Frontiers Media S.A. 2023-01-24 /pmc/articles/PMC9904361/ /pubmed/36762268 http://dx.doi.org/10.3389/ti.2023.11046 Text en Copyright © 2023 Planinc, Ilic, Dejea, Garcia-Canadilla, Gasparovic, Jurin, Milicic, Skoric, Stampanoni, Bijnens, Bonnin and Cikes. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Health Archive
Planinc, Ivo
Ilic, Ivana
Dejea, Hector
Garcia-Canadilla, Patricia
Gasparovic, Hrvoje
Jurin, Hrvoje
Milicic, Davor
Skoric, Bosko
Stampanoni, Marco
Bijnens, Bart
Bonnin, Anne
Cikes, Maja
A Novel Three-Dimensional Approach Towards Evaluating Endomyocardial Biopsies for Follow-Up After Heart Transplantation: X-Ray Phase Contrast Imaging and Its Agreement With Classical Histopathology
title A Novel Three-Dimensional Approach Towards Evaluating Endomyocardial Biopsies for Follow-Up After Heart Transplantation: X-Ray Phase Contrast Imaging and Its Agreement With Classical Histopathology
title_full A Novel Three-Dimensional Approach Towards Evaluating Endomyocardial Biopsies for Follow-Up After Heart Transplantation: X-Ray Phase Contrast Imaging and Its Agreement With Classical Histopathology
title_fullStr A Novel Three-Dimensional Approach Towards Evaluating Endomyocardial Biopsies for Follow-Up After Heart Transplantation: X-Ray Phase Contrast Imaging and Its Agreement With Classical Histopathology
title_full_unstemmed A Novel Three-Dimensional Approach Towards Evaluating Endomyocardial Biopsies for Follow-Up After Heart Transplantation: X-Ray Phase Contrast Imaging and Its Agreement With Classical Histopathology
title_short A Novel Three-Dimensional Approach Towards Evaluating Endomyocardial Biopsies for Follow-Up After Heart Transplantation: X-Ray Phase Contrast Imaging and Its Agreement With Classical Histopathology
title_sort novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology
topic Health Archive
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9904361/
https://www.ncbi.nlm.nih.gov/pubmed/36762268
http://dx.doi.org/10.3389/ti.2023.11046
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