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Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan
The molecular profile of hepatitis Delta in Afghanistan remains unclear yet, therefore this study addresses the genotype of HDV among HBsAg carriers in Afghanistan. In total 234 HBsAg-positive sera were examined by chemiluminescent micro-particle immunoassay to detect Anti-HDV antibodies. Serologica...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Shiraz University
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9905752/ https://www.ncbi.nlm.nih.gov/pubmed/36776998 http://dx.doi.org/10.22099/mbrc.2022.44692.1780 |
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author | Husseini, Abbas Ali Rostamzadeh, Mehran |
author_facet | Husseini, Abbas Ali Rostamzadeh, Mehran |
author_sort | Husseini, Abbas Ali |
collection | PubMed |
description | The molecular profile of hepatitis Delta in Afghanistan remains unclear yet, therefore this study addresses the genotype of HDV among HBsAg carriers in Afghanistan. In total 234 HBsAg-positive sera were examined by chemiluminescent micro-particle immunoassay to detect Anti-HDV antibodies. Serologically positive samples were later approved via real-time PCR test and subsequently, a 731 bp segment of the HDV Delta antigen RNA region was sequenced in the Illumina platform. The isolates were genotyped via distance matrix/UPGMA analysis using Kimura 2-parameter by MEGA7 software package program. The HBV/HDV coinfection rate among HBsAg carriers in Afghanistan was 2.1%. Finally, 4 samples successfully amplified Hepatitis delta antigen (HDAg) which Later in phylogenetic analysis, all resided in branch genotype I and were stored at GenBank with accession numbers MK799645, MK799646, MK799647, MK799648. The HDV genotypic variations in the Afghan HBsAg carriers may be homogenous and HDV-1 may be the predominant genotype in Afghanistan. |
format | Online Article Text |
id | pubmed-9905752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Shiraz University |
record_format | MEDLINE/PubMed |
spelling | pubmed-99057522023-02-09 Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan Husseini, Abbas Ali Rostamzadeh, Mehran Mol Biol Res Commun Short Communication The molecular profile of hepatitis Delta in Afghanistan remains unclear yet, therefore this study addresses the genotype of HDV among HBsAg carriers in Afghanistan. In total 234 HBsAg-positive sera were examined by chemiluminescent micro-particle immunoassay to detect Anti-HDV antibodies. Serologically positive samples were later approved via real-time PCR test and subsequently, a 731 bp segment of the HDV Delta antigen RNA region was sequenced in the Illumina platform. The isolates were genotyped via distance matrix/UPGMA analysis using Kimura 2-parameter by MEGA7 software package program. The HBV/HDV coinfection rate among HBsAg carriers in Afghanistan was 2.1%. Finally, 4 samples successfully amplified Hepatitis delta antigen (HDAg) which Later in phylogenetic analysis, all resided in branch genotype I and were stored at GenBank with accession numbers MK799645, MK799646, MK799647, MK799648. The HDV genotypic variations in the Afghan HBsAg carriers may be homogenous and HDV-1 may be the predominant genotype in Afghanistan. Shiraz University 2022 /pmc/articles/PMC9905752/ /pubmed/36776998 http://dx.doi.org/10.22099/mbrc.2022.44692.1780 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Husseini, Abbas Ali Rostamzadeh, Mehran Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan |
title | Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan |
title_full | Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan |
title_fullStr | Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan |
title_full_unstemmed | Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan |
title_short | Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan |
title_sort | phylogenetic analysis and prevalence of delta hepatitis among hbsag carriers in afghanistan |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9905752/ https://www.ncbi.nlm.nih.gov/pubmed/36776998 http://dx.doi.org/10.22099/mbrc.2022.44692.1780 |
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