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Current molecular approach for diagnosis of MRSA: a meta-narrative review

INTRODUCTION: Detection and diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) are important in ensuring a correct and effective treatment, further reducing its spread. A wide range of molecular approaches has been used for the diagnosis of antimicrobial resistance (AMR) in MRSA. This r...

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Autores principales: Xing, Sim Yi, Wei, Lee Qiao, Abushelaibi, Aisha, Lai, Kok Song, Lim, Swee Hua Erin, Maran, Sathiya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AboutScience 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906022/
https://www.ncbi.nlm.nih.gov/pubmed/36761068
http://dx.doi.org/10.33393/dti.2022.2522
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author Xing, Sim Yi
Wei, Lee Qiao
Abushelaibi, Aisha
Lai, Kok Song
Lim, Swee Hua Erin
Maran, Sathiya
author_facet Xing, Sim Yi
Wei, Lee Qiao
Abushelaibi, Aisha
Lai, Kok Song
Lim, Swee Hua Erin
Maran, Sathiya
author_sort Xing, Sim Yi
collection PubMed
description INTRODUCTION: Detection and diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) are important in ensuring a correct and effective treatment, further reducing its spread. A wide range of molecular approaches has been used for the diagnosis of antimicrobial resistance (AMR) in MRSA. This review aims to study and appraise widely used molecular diagnostic methods for detecting MRSA. METHODS: This meta-narrative review was performed by searching PubMed using the following search terms: (molecular diagnosis) AND (antimicrobial resistance) AND (methicillin-resistant Staphylococcus aureus). Studies using molecular diagnostic techniques for the detection of MRSA were included, while non-English language, duplicates and non-article studies were excluded. After reviewing the libraries and a further manual search, 20 studies were included in this article. RAMESES publication standard for narrative reviews was used for this synthesis. RESULTS: A total of 20 full papers were reviewed and appraised in this synthesis, consisting of PCR technique (n = 7), deoxyribonucleic acid (DNA) Microarray (n = 1), DNA sequencing (n = 2), Xpert MRSA/SA BC assay (n = 2), matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) (n = 2), MLST (n = 4), SCCmec typing (n = 1) and GENECUBE (n = 1). DISCUSSION: Different diagnostic methods used to diagnose MRSA have been studied in this review. This study concludes that PCR has been extensively used due to its higher sensitivity and cost-effectiveness in the past five years
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spelling pubmed-99060222023-02-08 Current molecular approach for diagnosis of MRSA: a meta-narrative review Xing, Sim Yi Wei, Lee Qiao Abushelaibi, Aisha Lai, Kok Song Lim, Swee Hua Erin Maran, Sathiya Drug Target Insights Review INTRODUCTION: Detection and diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) are important in ensuring a correct and effective treatment, further reducing its spread. A wide range of molecular approaches has been used for the diagnosis of antimicrobial resistance (AMR) in MRSA. This review aims to study and appraise widely used molecular diagnostic methods for detecting MRSA. METHODS: This meta-narrative review was performed by searching PubMed using the following search terms: (molecular diagnosis) AND (antimicrobial resistance) AND (methicillin-resistant Staphylococcus aureus). Studies using molecular diagnostic techniques for the detection of MRSA were included, while non-English language, duplicates and non-article studies were excluded. After reviewing the libraries and a further manual search, 20 studies were included in this article. RAMESES publication standard for narrative reviews was used for this synthesis. RESULTS: A total of 20 full papers were reviewed and appraised in this synthesis, consisting of PCR technique (n = 7), deoxyribonucleic acid (DNA) Microarray (n = 1), DNA sequencing (n = 2), Xpert MRSA/SA BC assay (n = 2), matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) (n = 2), MLST (n = 4), SCCmec typing (n = 1) and GENECUBE (n = 1). DISCUSSION: Different diagnostic methods used to diagnose MRSA have been studied in this review. This study concludes that PCR has been extensively used due to its higher sensitivity and cost-effectiveness in the past five years AboutScience 2022-12-31 /pmc/articles/PMC9906022/ /pubmed/36761068 http://dx.doi.org/10.33393/dti.2022.2522 Text en Copyright © 2022, The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/© 2022 The Authors. This article is published by AboutScience and licensed under Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Commercial use is not permitted and is subject to Publisher’s permissions. Full information is available at www.aboutscience.eu
spellingShingle Review
Xing, Sim Yi
Wei, Lee Qiao
Abushelaibi, Aisha
Lai, Kok Song
Lim, Swee Hua Erin
Maran, Sathiya
Current molecular approach for diagnosis of MRSA: a meta-narrative review
title Current molecular approach for diagnosis of MRSA: a meta-narrative review
title_full Current molecular approach for diagnosis of MRSA: a meta-narrative review
title_fullStr Current molecular approach for diagnosis of MRSA: a meta-narrative review
title_full_unstemmed Current molecular approach for diagnosis of MRSA: a meta-narrative review
title_short Current molecular approach for diagnosis of MRSA: a meta-narrative review
title_sort current molecular approach for diagnosis of mrsa: a meta-narrative review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906022/
https://www.ncbi.nlm.nih.gov/pubmed/36761068
http://dx.doi.org/10.33393/dti.2022.2522
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