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The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress
Eukaryotic genomes are duplicated from thousands of replication origins that fire sequentially forming a defined spatiotemporal pattern of replication clusters. The temporal order of DNA replication is determined by chromatin architecture and, more specifically, by chromatin contacts that are stabil...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906048/ https://www.ncbi.nlm.nih.gov/pubmed/36746532 http://dx.doi.org/10.26508/lsa.202101186 |
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author | Lebdy, Rana Patouillard, Julie Larroque, Marion Urbach, Serge Abou Merhi, Raghida Larroque, Christian Ribeyre, Cyril |
author_facet | Lebdy, Rana Patouillard, Julie Larroque, Marion Urbach, Serge Abou Merhi, Raghida Larroque, Christian Ribeyre, Cyril |
author_sort | Lebdy, Rana |
collection | PubMed |
description | Eukaryotic genomes are duplicated from thousands of replication origins that fire sequentially forming a defined spatiotemporal pattern of replication clusters. The temporal order of DNA replication is determined by chromatin architecture and, more specifically, by chromatin contacts that are stabilized by RIF1. Here, we show that RIF1 localizes near newly synthesized DNA. In cells exposed to the DNA replication inhibitor aphidicolin, suppression of RIF1 markedly decreased the efficacy of isolation of proteins on nascent DNA, suggesting that the isolation of proteins on nascent DNA procedure is biased by chromatin topology. RIF1 was required to limit the accumulation of DNA lesions induced by aphidicolin treatment and promoted the recruitment of cohesins in the vicinity of nascent DNA. Collectively, the data suggest that the stabilization of chromatin topology by RIF1 limits replication-associated genomic instability. |
format | Online Article Text |
id | pubmed-9906048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-99060482023-02-08 The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress Lebdy, Rana Patouillard, Julie Larroque, Marion Urbach, Serge Abou Merhi, Raghida Larroque, Christian Ribeyre, Cyril Life Sci Alliance Research Articles Eukaryotic genomes are duplicated from thousands of replication origins that fire sequentially forming a defined spatiotemporal pattern of replication clusters. The temporal order of DNA replication is determined by chromatin architecture and, more specifically, by chromatin contacts that are stabilized by RIF1. Here, we show that RIF1 localizes near newly synthesized DNA. In cells exposed to the DNA replication inhibitor aphidicolin, suppression of RIF1 markedly decreased the efficacy of isolation of proteins on nascent DNA, suggesting that the isolation of proteins on nascent DNA procedure is biased by chromatin topology. RIF1 was required to limit the accumulation of DNA lesions induced by aphidicolin treatment and promoted the recruitment of cohesins in the vicinity of nascent DNA. Collectively, the data suggest that the stabilization of chromatin topology by RIF1 limits replication-associated genomic instability. Life Science Alliance LLC 2023-02-06 /pmc/articles/PMC9906048/ /pubmed/36746532 http://dx.doi.org/10.26508/lsa.202101186 Text en © 2023 Lebdy et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Lebdy, Rana Patouillard, Julie Larroque, Marion Urbach, Serge Abou Merhi, Raghida Larroque, Christian Ribeyre, Cyril The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress |
title | The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress |
title_full | The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress |
title_fullStr | The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress |
title_full_unstemmed | The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress |
title_short | The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress |
title_sort | organizer of chromatin topology rif1 ensures cellular resilience to dna replication stress |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906048/ https://www.ncbi.nlm.nih.gov/pubmed/36746532 http://dx.doi.org/10.26508/lsa.202101186 |
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