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miR-135a inhibits the proliferation of HBV-infected hepatocellular carcinoma cells by targeting HOXA10
BACKGROUND: The incidence of hepatocellular carcinoma (HCC) in patients with hepatitis B virus (HBV) is extremely high. MicroRNAs (miRNAs) are a type of endogenous non-coding small RNA with novel molecular therapeutic mechanisms that plays an important role in the occurrence and development of cance...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906062/ https://www.ncbi.nlm.nih.gov/pubmed/36760373 http://dx.doi.org/10.21037/tcr-22-2789 |
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author | Lin, Jianjun Lian, Xiang Xue, Shihang Ouyang, Lian Zhou, Lihui Lu, Yuyang Xie, Longteng |
author_facet | Lin, Jianjun Lian, Xiang Xue, Shihang Ouyang, Lian Zhou, Lihui Lu, Yuyang Xie, Longteng |
author_sort | Lin, Jianjun |
collection | PubMed |
description | BACKGROUND: The incidence of hepatocellular carcinoma (HCC) in patients with hepatitis B virus (HBV) is extremely high. MicroRNAs (miRNAs) are a type of endogenous non-coding small RNA with novel molecular therapeutic mechanisms that plays an important role in the occurrence and development of cancers. This study aimed to explore the regulation mechanism of miR-135a and HOXA10 in the proliferation, invasion, and apoptosis of HCC cells. METHODS: Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis was used to detect the expression level of miR-135a. Overexpression of miR-135a was used to measure the roles of miR-135a in the proliferation, invasion, and apoptosis of HCC cells. A dual luciferase experiment was performed to assess the relationship between HOXA10 and miR-135a. Western blot was applied to observe the protein levels of p-p38, p-ERK, and p-JNK. RESULTS: The expression levels of miR-135a were significantly decreased in HCC tissues and cells. Overexpression of miR-135a inhibited the proliferation and invasion but promoted the apoptosis of HCC cells. Importantly, our results confirmed that HOXA10 was a direct target of miR-135a. Under HBV infection, silencing of HOXA10 significantly blocked the proliferation and invasion and promoted the apoptosis of HCC cells. In addition, miR-135a/HOXA10 regulated the expressions of p-p38, p-ERK, and p-JNK through the miR-135a/HOXA10 axis, thereby inhibiting the activation of the MAPK pathway. CONCLUSIONS: HBV promoted the proliferation and invasion, and inhibited the apoptosis of HCC cells by regulating the miR-135a/HOXA10 pathway. These findings provide a theoretical basis for improving the treatment of HBV-infected HCC patients. |
format | Online Article Text |
id | pubmed-9906062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-99060622023-02-08 miR-135a inhibits the proliferation of HBV-infected hepatocellular carcinoma cells by targeting HOXA10 Lin, Jianjun Lian, Xiang Xue, Shihang Ouyang, Lian Zhou, Lihui Lu, Yuyang Xie, Longteng Transl Cancer Res Original Article BACKGROUND: The incidence of hepatocellular carcinoma (HCC) in patients with hepatitis B virus (HBV) is extremely high. MicroRNAs (miRNAs) are a type of endogenous non-coding small RNA with novel molecular therapeutic mechanisms that plays an important role in the occurrence and development of cancers. This study aimed to explore the regulation mechanism of miR-135a and HOXA10 in the proliferation, invasion, and apoptosis of HCC cells. METHODS: Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis was used to detect the expression level of miR-135a. Overexpression of miR-135a was used to measure the roles of miR-135a in the proliferation, invasion, and apoptosis of HCC cells. A dual luciferase experiment was performed to assess the relationship between HOXA10 and miR-135a. Western blot was applied to observe the protein levels of p-p38, p-ERK, and p-JNK. RESULTS: The expression levels of miR-135a were significantly decreased in HCC tissues and cells. Overexpression of miR-135a inhibited the proliferation and invasion but promoted the apoptosis of HCC cells. Importantly, our results confirmed that HOXA10 was a direct target of miR-135a. Under HBV infection, silencing of HOXA10 significantly blocked the proliferation and invasion and promoted the apoptosis of HCC cells. In addition, miR-135a/HOXA10 regulated the expressions of p-p38, p-ERK, and p-JNK through the miR-135a/HOXA10 axis, thereby inhibiting the activation of the MAPK pathway. CONCLUSIONS: HBV promoted the proliferation and invasion, and inhibited the apoptosis of HCC cells by regulating the miR-135a/HOXA10 pathway. These findings provide a theoretical basis for improving the treatment of HBV-infected HCC patients. AME Publishing Company 2023-01-16 2023-01-30 /pmc/articles/PMC9906062/ /pubmed/36760373 http://dx.doi.org/10.21037/tcr-22-2789 Text en 2023 Translational Cancer Research. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Lin, Jianjun Lian, Xiang Xue, Shihang Ouyang, Lian Zhou, Lihui Lu, Yuyang Xie, Longteng miR-135a inhibits the proliferation of HBV-infected hepatocellular carcinoma cells by targeting HOXA10 |
title | miR-135a inhibits the proliferation of HBV-infected hepatocellular carcinoma cells by targeting HOXA10 |
title_full | miR-135a inhibits the proliferation of HBV-infected hepatocellular carcinoma cells by targeting HOXA10 |
title_fullStr | miR-135a inhibits the proliferation of HBV-infected hepatocellular carcinoma cells by targeting HOXA10 |
title_full_unstemmed | miR-135a inhibits the proliferation of HBV-infected hepatocellular carcinoma cells by targeting HOXA10 |
title_short | miR-135a inhibits the proliferation of HBV-infected hepatocellular carcinoma cells by targeting HOXA10 |
title_sort | mir-135a inhibits the proliferation of hbv-infected hepatocellular carcinoma cells by targeting hoxa10 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906062/ https://www.ncbi.nlm.nih.gov/pubmed/36760373 http://dx.doi.org/10.21037/tcr-22-2789 |
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