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Finding a vocation for validation: taking proteomics beyond association and location

First established in the seventies, proteomics, chemoproteomics, and most recently, spatial/proximity-proteomics technologies have empowered researchers with new capabilities to illuminate cellular communication networks that govern sophisticated decision-making processes. With an ever-growing inven...

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Detalles Bibliográficos
Autores principales: Long, Marcus J. C., Liu, Jinmin, Aye, Yimon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906375/
https://www.ncbi.nlm.nih.gov/pubmed/36794020
http://dx.doi.org/10.1039/d2cb00214k
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author Long, Marcus J. C.
Liu, Jinmin
Aye, Yimon
author_facet Long, Marcus J. C.
Liu, Jinmin
Aye, Yimon
author_sort Long, Marcus J. C.
collection PubMed
description First established in the seventies, proteomics, chemoproteomics, and most recently, spatial/proximity-proteomics technologies have empowered researchers with new capabilities to illuminate cellular communication networks that govern sophisticated decision-making processes. With an ever-growing inventory of these advanced proteomics tools, the onus is upon the researchers to understand their individual advantages and limitations, such that we can ensure rigorous implementation and conclusions derived from critical data interpretations backed up by orthogonal series of functional validations. This perspective—based on the authors’ experience in applying varied proteomics workflows in complex living models—underlines key book-keeping considerations, comparing and contrasting most-commonly-deployed modern proteomics profiling technologies. We hope this article stimulates thoughts among expert users and equips new-comers with practical knowhow of what has become an indispensable tool in chemical biology, drug discovery, and broader life-science investigations.
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spelling pubmed-99063752023-02-14 Finding a vocation for validation: taking proteomics beyond association and location Long, Marcus J. C. Liu, Jinmin Aye, Yimon RSC Chem Biol Chemistry First established in the seventies, proteomics, chemoproteomics, and most recently, spatial/proximity-proteomics technologies have empowered researchers with new capabilities to illuminate cellular communication networks that govern sophisticated decision-making processes. With an ever-growing inventory of these advanced proteomics tools, the onus is upon the researchers to understand their individual advantages and limitations, such that we can ensure rigorous implementation and conclusions derived from critical data interpretations backed up by orthogonal series of functional validations. This perspective—based on the authors’ experience in applying varied proteomics workflows in complex living models—underlines key book-keeping considerations, comparing and contrasting most-commonly-deployed modern proteomics profiling technologies. We hope this article stimulates thoughts among expert users and equips new-comers with practical knowhow of what has become an indispensable tool in chemical biology, drug discovery, and broader life-science investigations. RSC 2022-12-02 /pmc/articles/PMC9906375/ /pubmed/36794020 http://dx.doi.org/10.1039/d2cb00214k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Long, Marcus J. C.
Liu, Jinmin
Aye, Yimon
Finding a vocation for validation: taking proteomics beyond association and location
title Finding a vocation for validation: taking proteomics beyond association and location
title_full Finding a vocation for validation: taking proteomics beyond association and location
title_fullStr Finding a vocation for validation: taking proteomics beyond association and location
title_full_unstemmed Finding a vocation for validation: taking proteomics beyond association and location
title_short Finding a vocation for validation: taking proteomics beyond association and location
title_sort finding a vocation for validation: taking proteomics beyond association and location
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906375/
https://www.ncbi.nlm.nih.gov/pubmed/36794020
http://dx.doi.org/10.1039/d2cb00214k
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