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Finding a vocation for validation: taking proteomics beyond association and location
First established in the seventies, proteomics, chemoproteomics, and most recently, spatial/proximity-proteomics technologies have empowered researchers with new capabilities to illuminate cellular communication networks that govern sophisticated decision-making processes. With an ever-growing inven...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906375/ https://www.ncbi.nlm.nih.gov/pubmed/36794020 http://dx.doi.org/10.1039/d2cb00214k |
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author | Long, Marcus J. C. Liu, Jinmin Aye, Yimon |
author_facet | Long, Marcus J. C. Liu, Jinmin Aye, Yimon |
author_sort | Long, Marcus J. C. |
collection | PubMed |
description | First established in the seventies, proteomics, chemoproteomics, and most recently, spatial/proximity-proteomics technologies have empowered researchers with new capabilities to illuminate cellular communication networks that govern sophisticated decision-making processes. With an ever-growing inventory of these advanced proteomics tools, the onus is upon the researchers to understand their individual advantages and limitations, such that we can ensure rigorous implementation and conclusions derived from critical data interpretations backed up by orthogonal series of functional validations. This perspective—based on the authors’ experience in applying varied proteomics workflows in complex living models—underlines key book-keeping considerations, comparing and contrasting most-commonly-deployed modern proteomics profiling technologies. We hope this article stimulates thoughts among expert users and equips new-comers with practical knowhow of what has become an indispensable tool in chemical biology, drug discovery, and broader life-science investigations. |
format | Online Article Text |
id | pubmed-9906375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | RSC |
record_format | MEDLINE/PubMed |
spelling | pubmed-99063752023-02-14 Finding a vocation for validation: taking proteomics beyond association and location Long, Marcus J. C. Liu, Jinmin Aye, Yimon RSC Chem Biol Chemistry First established in the seventies, proteomics, chemoproteomics, and most recently, spatial/proximity-proteomics technologies have empowered researchers with new capabilities to illuminate cellular communication networks that govern sophisticated decision-making processes. With an ever-growing inventory of these advanced proteomics tools, the onus is upon the researchers to understand their individual advantages and limitations, such that we can ensure rigorous implementation and conclusions derived from critical data interpretations backed up by orthogonal series of functional validations. This perspective—based on the authors’ experience in applying varied proteomics workflows in complex living models—underlines key book-keeping considerations, comparing and contrasting most-commonly-deployed modern proteomics profiling technologies. We hope this article stimulates thoughts among expert users and equips new-comers with practical knowhow of what has become an indispensable tool in chemical biology, drug discovery, and broader life-science investigations. RSC 2022-12-02 /pmc/articles/PMC9906375/ /pubmed/36794020 http://dx.doi.org/10.1039/d2cb00214k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Long, Marcus J. C. Liu, Jinmin Aye, Yimon Finding a vocation for validation: taking proteomics beyond association and location |
title | Finding a vocation for validation: taking proteomics beyond association and location |
title_full | Finding a vocation for validation: taking proteomics beyond association and location |
title_fullStr | Finding a vocation for validation: taking proteomics beyond association and location |
title_full_unstemmed | Finding a vocation for validation: taking proteomics beyond association and location |
title_short | Finding a vocation for validation: taking proteomics beyond association and location |
title_sort | finding a vocation for validation: taking proteomics beyond association and location |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906375/ https://www.ncbi.nlm.nih.gov/pubmed/36794020 http://dx.doi.org/10.1039/d2cb00214k |
work_keys_str_mv | AT longmarcusjc findingavocationforvalidationtakingproteomicsbeyondassociationandlocation AT liujinmin findingavocationforvalidationtakingproteomicsbeyondassociationandlocation AT ayeyimon findingavocationforvalidationtakingproteomicsbeyondassociationandlocation |