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Potential antiviral effects of pantethine against SARS-CoV-2
SARS-CoV-2 interacts with cellular cholesterol during many stages of its replication cycle. Pantethine was reported to reduce total cholesterol levels and fatty acid synthesis and potentially alter different processes that might be involved in the SARS-CoV-2 replication cycle. Here, we explored the...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906591/ https://www.ncbi.nlm.nih.gov/pubmed/36754974 http://dx.doi.org/10.1038/s41598-023-29245-0 |
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author | Abou-Hamdan, M. Saleh, R. Mani, S. Dournaud, P. Metifiot, M. Blondot, M. L. Andreola, M. L. Abdel-sater, F. De Reggi, M. Gressens, P. Laforge, M. |
author_facet | Abou-Hamdan, M. Saleh, R. Mani, S. Dournaud, P. Metifiot, M. Blondot, M. L. Andreola, M. L. Abdel-sater, F. De Reggi, M. Gressens, P. Laforge, M. |
author_sort | Abou-Hamdan, M. |
collection | PubMed |
description | SARS-CoV-2 interacts with cellular cholesterol during many stages of its replication cycle. Pantethine was reported to reduce total cholesterol levels and fatty acid synthesis and potentially alter different processes that might be involved in the SARS-CoV-2 replication cycle. Here, we explored the potential antiviral effects of pantethine in two in vitro experimental models of SARS-CoV-2 infection, in Vero E6 cells and in Calu-3a cells. Pantethine reduced the infection of cells by SARS-CoV-2 in both preinfection and postinfection treatment regimens. Accordingly, cellular expression of the viral spike and nucleocapsid proteins was substantially reduced, and we observed a significant reduction in viral copy numbers in the supernatant of cells treated with pantethine. In addition, pantethine inhibited the infection-induced increase in TMPRSS2 and HECT E3 ligase expression in infected cells as well as the increase in antiviral interferon-beta response and inflammatory gene expression in Calu-3a cells. Our results demonstrate that pantethine, which is well tolerated in humans, was very effective in controlling SARS-CoV-2 infection and might represent a new therapeutic drug that can be repurposed for the prevention or treatment of COVID-19 and long COVID syndrome. |
format | Online Article Text |
id | pubmed-9906591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-99065912023-02-08 Potential antiviral effects of pantethine against SARS-CoV-2 Abou-Hamdan, M. Saleh, R. Mani, S. Dournaud, P. Metifiot, M. Blondot, M. L. Andreola, M. L. Abdel-sater, F. De Reggi, M. Gressens, P. Laforge, M. Sci Rep Article SARS-CoV-2 interacts with cellular cholesterol during many stages of its replication cycle. Pantethine was reported to reduce total cholesterol levels and fatty acid synthesis and potentially alter different processes that might be involved in the SARS-CoV-2 replication cycle. Here, we explored the potential antiviral effects of pantethine in two in vitro experimental models of SARS-CoV-2 infection, in Vero E6 cells and in Calu-3a cells. Pantethine reduced the infection of cells by SARS-CoV-2 in both preinfection and postinfection treatment regimens. Accordingly, cellular expression of the viral spike and nucleocapsid proteins was substantially reduced, and we observed a significant reduction in viral copy numbers in the supernatant of cells treated with pantethine. In addition, pantethine inhibited the infection-induced increase in TMPRSS2 and HECT E3 ligase expression in infected cells as well as the increase in antiviral interferon-beta response and inflammatory gene expression in Calu-3a cells. Our results demonstrate that pantethine, which is well tolerated in humans, was very effective in controlling SARS-CoV-2 infection and might represent a new therapeutic drug that can be repurposed for the prevention or treatment of COVID-19 and long COVID syndrome. Nature Publishing Group UK 2023-02-08 /pmc/articles/PMC9906591/ /pubmed/36754974 http://dx.doi.org/10.1038/s41598-023-29245-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Abou-Hamdan, M. Saleh, R. Mani, S. Dournaud, P. Metifiot, M. Blondot, M. L. Andreola, M. L. Abdel-sater, F. De Reggi, M. Gressens, P. Laforge, M. Potential antiviral effects of pantethine against SARS-CoV-2 |
title | Potential antiviral effects of pantethine against SARS-CoV-2 |
title_full | Potential antiviral effects of pantethine against SARS-CoV-2 |
title_fullStr | Potential antiviral effects of pantethine against SARS-CoV-2 |
title_full_unstemmed | Potential antiviral effects of pantethine against SARS-CoV-2 |
title_short | Potential antiviral effects of pantethine against SARS-CoV-2 |
title_sort | potential antiviral effects of pantethine against sars-cov-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906591/ https://www.ncbi.nlm.nih.gov/pubmed/36754974 http://dx.doi.org/10.1038/s41598-023-29245-0 |
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