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Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor
BACKGROUND: Decreased calcium-sensing receptor (CaSR) has been observed in hyperparathyroidism (HPT) without a known mechanism. The purpose of this study was to evaluate the expression of CaSR in primary (PHPT) and secondary (SHPT) subtypes. METHODS: Immunohistochemical (IHC) staining and quantitati...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906946/ https://www.ncbi.nlm.nih.gov/pubmed/36755240 http://dx.doi.org/10.1186/s12893-023-01928-5 |
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author | Li, Xin Lu, Yao Zhang, Ling Song, Aiping Zhang, Honglei Pang, Bo Liu, Jun Sun, Xiaoliang Ji, Haoyang Huang, Linping Yang, Meng |
author_facet | Li, Xin Lu, Yao Zhang, Ling Song, Aiping Zhang, Honglei Pang, Bo Liu, Jun Sun, Xiaoliang Ji, Haoyang Huang, Linping Yang, Meng |
author_sort | Li, Xin |
collection | PubMed |
description | BACKGROUND: Decreased calcium-sensing receptor (CaSR) has been observed in hyperparathyroidism (HPT) without a known mechanism. The purpose of this study was to evaluate the expression of CaSR in primary (PHPT) and secondary (SHPT) subtypes. METHODS: Immunohistochemical (IHC) staining and quantitative real-time PCR (qRT-PCR) assay were used to measure the differences in expression of CaSR protein and gene in PHPT and SHPT human samples, compared to matched controls. RESULTS: CaSR protein was differentially downregulated in SHPT and PHPT compared to normal parathyroid tissues (2.42 ± 0.5 vs. 3.2 ± 0.62, P < 0.05; 1.8 ± 0.83 vs. 3.2 ± 0.62, P < 0.05, respectively). Furthermore, SHPT tissues exhibited significantly higher levels of CaSR mRNA (0.29 ± 0.23 vs. 0.01 ± 0.12, P < 0.05) and protein (2.42 ± 0.5 vs. 1.8 ± 0.83, P < 0.05) than those in PHPT tissue samples. CONCLUSION: Depressed CaSR expression was a critical pathological hallmark of HPT. We found a differential decline of CaSR, in terms of both mRNA and protein levels, in PHPT and SHPT human samples. We think that CaSR dysregulation occurred at the very beginning of disease onset in PHPT, while a similar pathological scenario appeared at the later stage of SHPT. Future studies should be directed to dissect the mechanistic involvement of CaSR in PHPT and SHPT in order to bring treatment precisions in HPT management. |
format | Online Article Text |
id | pubmed-9906946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-99069462023-02-08 Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor Li, Xin Lu, Yao Zhang, Ling Song, Aiping Zhang, Honglei Pang, Bo Liu, Jun Sun, Xiaoliang Ji, Haoyang Huang, Linping Yang, Meng BMC Surg Research BACKGROUND: Decreased calcium-sensing receptor (CaSR) has been observed in hyperparathyroidism (HPT) without a known mechanism. The purpose of this study was to evaluate the expression of CaSR in primary (PHPT) and secondary (SHPT) subtypes. METHODS: Immunohistochemical (IHC) staining and quantitative real-time PCR (qRT-PCR) assay were used to measure the differences in expression of CaSR protein and gene in PHPT and SHPT human samples, compared to matched controls. RESULTS: CaSR protein was differentially downregulated in SHPT and PHPT compared to normal parathyroid tissues (2.42 ± 0.5 vs. 3.2 ± 0.62, P < 0.05; 1.8 ± 0.83 vs. 3.2 ± 0.62, P < 0.05, respectively). Furthermore, SHPT tissues exhibited significantly higher levels of CaSR mRNA (0.29 ± 0.23 vs. 0.01 ± 0.12, P < 0.05) and protein (2.42 ± 0.5 vs. 1.8 ± 0.83, P < 0.05) than those in PHPT tissue samples. CONCLUSION: Depressed CaSR expression was a critical pathological hallmark of HPT. We found a differential decline of CaSR, in terms of both mRNA and protein levels, in PHPT and SHPT human samples. We think that CaSR dysregulation occurred at the very beginning of disease onset in PHPT, while a similar pathological scenario appeared at the later stage of SHPT. Future studies should be directed to dissect the mechanistic involvement of CaSR in PHPT and SHPT in order to bring treatment precisions in HPT management. BioMed Central 2023-02-08 /pmc/articles/PMC9906946/ /pubmed/36755240 http://dx.doi.org/10.1186/s12893-023-01928-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Li, Xin Lu, Yao Zhang, Ling Song, Aiping Zhang, Honglei Pang, Bo Liu, Jun Sun, Xiaoliang Ji, Haoyang Huang, Linping Yang, Meng Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
title | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
title_full | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
title_fullStr | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
title_full_unstemmed | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
title_short | Primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
title_sort | primary and secondary hyperparathyroidism present different expressions of calcium-sensing receptor |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9906946/ https://www.ncbi.nlm.nih.gov/pubmed/36755240 http://dx.doi.org/10.1186/s12893-023-01928-5 |
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