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Assessment of Treg-related lncRNAs in epilepsy

Recent studies have shown dysregulation of several groups of long non-coding RNAs in the context of epilepsy. According to evidence regarding the role of regulatory T cells in this disorder, we examined expression levels of regulatory T cell-related lncRNAs, namely TH2-LCR, RMRP, IFNG-AS1 (NEST), MA...

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Autores principales: Sharifi, Guive, Eghtedarian, Reyhane, Taheri, Mohammad, Hussen, Bashdar Mahmud, Eslami, Solat, Ghafouri-Fard, Soudeh, Sayad, Arezou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9908604/
https://www.ncbi.nlm.nih.gov/pubmed/36776769
http://dx.doi.org/10.3389/fnmol.2022.1031314
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author Sharifi, Guive
Eghtedarian, Reyhane
Taheri, Mohammad
Hussen, Bashdar Mahmud
Eslami, Solat
Ghafouri-Fard, Soudeh
Sayad, Arezou
author_facet Sharifi, Guive
Eghtedarian, Reyhane
Taheri, Mohammad
Hussen, Bashdar Mahmud
Eslami, Solat
Ghafouri-Fard, Soudeh
Sayad, Arezou
author_sort Sharifi, Guive
collection PubMed
description Recent studies have shown dysregulation of several groups of long non-coding RNAs in the context of epilepsy. According to evidence regarding the role of regulatory T cells in this disorder, we examined expression levels of regulatory T cell-related lncRNAs, namely TH2-LCR, RMRP, IFNG-AS1 (NEST), MAFTRR and FLICR in the blood of epileptic cases compared with controls. Expression of RMRP was lower in patients with refractory epilepsy compared with controls [expression ratio (95% CI) = 0.32 (0.13–0.8), adjusted p-value = 0.0008]. Besides, its expression was lower in refractory patients vs. non-refractory patients [expression ratio (95% CI) = 0.2 (0.1–0.41), adjusted p-value < 0.0001]. Expression of TH2-LCR was lower in refractory patients vs. controls [expression ratio (95% CI) = 0.4 (0.17–0.93), adjusted p-value = 0.0044] and in refractory patients vs. non-refractory ones [Expression ratio = 0.28 (0.19–0.58), p-value < 0.0001]. Expression of NEST was higher in total patients [expression ratio (95% CI) = 2.48 (1.15–5.27), adjusted p-value = 0.0012] and in both groups of patients compared with controls. However, its expression was not different between refractory and non-refractory cases. Similarly, FLICR and MAFTRR were over-expressed in total cases and both groups of patients compared with controls, but their expressions were similar between refractory and non-refractory cases. MAFTRR could differentiate between total epileptic cases and controls with AUC value of 0.8. This lncRNA could separate refractory and non-refractory cases from healthy controls with AUC values of 0.73 and 0.88, respectively. This study provides evidence for deregulation of regulatory T cell-related lncRNAs in epilepsy and their potential role as diagnostic markers in this condition.
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spelling pubmed-99086042023-02-10 Assessment of Treg-related lncRNAs in epilepsy Sharifi, Guive Eghtedarian, Reyhane Taheri, Mohammad Hussen, Bashdar Mahmud Eslami, Solat Ghafouri-Fard, Soudeh Sayad, Arezou Front Mol Neurosci Molecular Neuroscience Recent studies have shown dysregulation of several groups of long non-coding RNAs in the context of epilepsy. According to evidence regarding the role of regulatory T cells in this disorder, we examined expression levels of regulatory T cell-related lncRNAs, namely TH2-LCR, RMRP, IFNG-AS1 (NEST), MAFTRR and FLICR in the blood of epileptic cases compared with controls. Expression of RMRP was lower in patients with refractory epilepsy compared with controls [expression ratio (95% CI) = 0.32 (0.13–0.8), adjusted p-value = 0.0008]. Besides, its expression was lower in refractory patients vs. non-refractory patients [expression ratio (95% CI) = 0.2 (0.1–0.41), adjusted p-value < 0.0001]. Expression of TH2-LCR was lower in refractory patients vs. controls [expression ratio (95% CI) = 0.4 (0.17–0.93), adjusted p-value = 0.0044] and in refractory patients vs. non-refractory ones [Expression ratio = 0.28 (0.19–0.58), p-value < 0.0001]. Expression of NEST was higher in total patients [expression ratio (95% CI) = 2.48 (1.15–5.27), adjusted p-value = 0.0012] and in both groups of patients compared with controls. However, its expression was not different between refractory and non-refractory cases. Similarly, FLICR and MAFTRR were over-expressed in total cases and both groups of patients compared with controls, but their expressions were similar between refractory and non-refractory cases. MAFTRR could differentiate between total epileptic cases and controls with AUC value of 0.8. This lncRNA could separate refractory and non-refractory cases from healthy controls with AUC values of 0.73 and 0.88, respectively. This study provides evidence for deregulation of regulatory T cell-related lncRNAs in epilepsy and their potential role as diagnostic markers in this condition. Frontiers Media S.A. 2023-01-26 /pmc/articles/PMC9908604/ /pubmed/36776769 http://dx.doi.org/10.3389/fnmol.2022.1031314 Text en Copyright © 2023 Sharifi, Eghtedarian, Taheri, Hussen, Eslami, Ghafouri-Fard and Sayad. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Sharifi, Guive
Eghtedarian, Reyhane
Taheri, Mohammad
Hussen, Bashdar Mahmud
Eslami, Solat
Ghafouri-Fard, Soudeh
Sayad, Arezou
Assessment of Treg-related lncRNAs in epilepsy
title Assessment of Treg-related lncRNAs in epilepsy
title_full Assessment of Treg-related lncRNAs in epilepsy
title_fullStr Assessment of Treg-related lncRNAs in epilepsy
title_full_unstemmed Assessment of Treg-related lncRNAs in epilepsy
title_short Assessment of Treg-related lncRNAs in epilepsy
title_sort assessment of treg-related lncrnas in epilepsy
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9908604/
https://www.ncbi.nlm.nih.gov/pubmed/36776769
http://dx.doi.org/10.3389/fnmol.2022.1031314
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