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A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes

CRISPR/Cas-based genome editing has dramatically improved genetic modification technology. In situ electroporation called genome editing via oviductal nucleic acid delivery (GONAD), which eliminates the need for ex vivo embryo handling, is technically the simplest method for gene transfer and can be...

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Detalles Bibliográficos
Autores principales:	Abe, Manabu, Nakatsukasa, Ena, Natsume, Rie, Hamada, Shun, Sakimura, Kenji, Watabe, Ayako M., Ohtsuka, Toshihisa
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9908863/ https://www.ncbi.nlm.nih.gov/pubmed/36755180 http://dx.doi.org/10.1038/s41598-023-29468-1

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