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Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro

OBJECTIVE: Lidocaine is an amide local anaesthetic commonly used for pain control, however, few studies have investigated the effect of lidocaine on the osteogenic differentiation of human dental pulp stem cells (HDPSCs). The present study aimed to determine the effect of lidocaine on HDPSC viabilit...

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Autores principales: Kim, Eun-Jung, Yoon, Ji-Uk, Kim, Cheul-Hong, Yoon, Ji-Young, Kim, Joo-Young, Kim, Hyang-Sook, Choi, Eun-Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9909061/
https://www.ncbi.nlm.nih.gov/pubmed/36748349
http://dx.doi.org/10.1177/03000605231152100
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author Kim, Eun-Jung
Yoon, Ji-Uk
Kim, Cheul-Hong
Yoon, Ji-Young
Kim, Joo-Young
Kim, Hyang-Sook
Choi, Eun-Ji
author_facet Kim, Eun-Jung
Yoon, Ji-Uk
Kim, Cheul-Hong
Yoon, Ji-Young
Kim, Joo-Young
Kim, Hyang-Sook
Choi, Eun-Ji
author_sort Kim, Eun-Jung
collection PubMed
description OBJECTIVE: Lidocaine is an amide local anaesthetic commonly used for pain control, however, few studies have investigated the effect of lidocaine on the osteogenic differentiation of human dental pulp stem cells (HDPSCs). The present study aimed to determine the effect of lidocaine on HDPSC viability and osteogenic differentiation. METHODS: HDPSCs were incubated with 0, 0.05, 0.2, 0.5, and 1 mM lidocaine for 24, 48 and 72 h, after which, MTT assays were performed. HDPSCs cultured with the above lidocaine concentrations and osteogenic differentiation medium for 7 and 14 days were stained for alkaline phosphatase (ALP). Protein and mRNA levels of relevant osteogenic factors (bone morphogenetic protein-2 [BMP-2] and runt-related transcription factor 2 [RUNX2]) were examined using western blotting and real-time reverse-transcription polymerase chain reaction, respectively. RESULTS: Lidocaine did not affect the viability of HDPSCs, however, lidocaine reduced ALP activity in HDPSCs. Levels of ALP, BMP-2, and RUNX2 mRNA were reduced with lidocaine, and levels of BMP-2 and RUNX2 proteins were decreased, versus controls. CONCLUSIONS: Lidocaine inhibits osteogenic differentiation markers in HDPSCs in vitro, even at low concentrations, without cytotoxicity. This study suggests that lidocaine may inhibit osteogenic differentiation in HDPSC-mediated regenerative medicine, including pulp regeneration and repair.
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spelling pubmed-99090612023-02-10 Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro Kim, Eun-Jung Yoon, Ji-Uk Kim, Cheul-Hong Yoon, Ji-Young Kim, Joo-Young Kim, Hyang-Sook Choi, Eun-Ji J Int Med Res Pre-Clinical Research Report OBJECTIVE: Lidocaine is an amide local anaesthetic commonly used for pain control, however, few studies have investigated the effect of lidocaine on the osteogenic differentiation of human dental pulp stem cells (HDPSCs). The present study aimed to determine the effect of lidocaine on HDPSC viability and osteogenic differentiation. METHODS: HDPSCs were incubated with 0, 0.05, 0.2, 0.5, and 1 mM lidocaine for 24, 48 and 72 h, after which, MTT assays were performed. HDPSCs cultured with the above lidocaine concentrations and osteogenic differentiation medium for 7 and 14 days were stained for alkaline phosphatase (ALP). Protein and mRNA levels of relevant osteogenic factors (bone morphogenetic protein-2 [BMP-2] and runt-related transcription factor 2 [RUNX2]) were examined using western blotting and real-time reverse-transcription polymerase chain reaction, respectively. RESULTS: Lidocaine did not affect the viability of HDPSCs, however, lidocaine reduced ALP activity in HDPSCs. Levels of ALP, BMP-2, and RUNX2 mRNA were reduced with lidocaine, and levels of BMP-2 and RUNX2 proteins were decreased, versus controls. CONCLUSIONS: Lidocaine inhibits osteogenic differentiation markers in HDPSCs in vitro, even at low concentrations, without cytotoxicity. This study suggests that lidocaine may inhibit osteogenic differentiation in HDPSC-mediated regenerative medicine, including pulp regeneration and repair. SAGE Publications 2023-02-07 /pmc/articles/PMC9909061/ /pubmed/36748349 http://dx.doi.org/10.1177/03000605231152100 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Pre-Clinical Research Report
Kim, Eun-Jung
Yoon, Ji-Uk
Kim, Cheul-Hong
Yoon, Ji-Young
Kim, Joo-Young
Kim, Hyang-Sook
Choi, Eun-Ji
Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro
title Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro
title_full Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro
title_fullStr Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro
title_full_unstemmed Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro
title_short Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro
title_sort lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro
topic Pre-Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9909061/
https://www.ncbi.nlm.nih.gov/pubmed/36748349
http://dx.doi.org/10.1177/03000605231152100
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