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The residues 4 to 6 at the N-terminus in particular modulate fibril propagation of β (2)-microglobulin : N-terminal modulates β2M fibril propagation

The ΔN6 truncation is the main posttranslational modification of β (2)-microglobulin (β (2)M) found in dialysis-related amyloid. Investigation of the interaction of wild-type (WT) β (2)M with N-terminally truncated variants is therefore of medical relevance. However, it is unclear which residues amo...

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Detalles Bibliográficos
Autores principales: Dang, Haibin, Chen, Zhixian, Chen, Wang, Luo, Xudong, Liu, Pan, Wang, Liqiang, Chen, Jie, Tang, Xuhai, Wang, Zhengzhi, Liang, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9909321/
https://www.ncbi.nlm.nih.gov/pubmed/35130623
http://dx.doi.org/10.3724/abbs.2021017
Descripción
Sumario:The ΔN6 truncation is the main posttranslational modification of β (2)-microglobulin (β (2)M) found in dialysis-related amyloid. Investigation of the interaction of wild-type (WT) β (2)M with N-terminally truncated variants is therefore of medical relevance. However, it is unclear which residues among the six residues at the N-terminus are crucial to the interactions and the modulation of amyloid fibril propagation of β (2)M. We herein analyzed homo- and heterotypic seeding of amyloid fibrils of WT human β (2)M and its N-terminally-truncated variants ΔN1 to ΔN6, lacking up to six residues at the N-terminus. At acidic pH 2.5, we produced amyloid fibrils in vitro from recombinant, WT β (2)M and its six truncated variants, and found that ΔN6 β (2)M fibrils exhibit a significantly lower conformational stability than WT β (2)M fibrils. Importantly, under more physiological conditions (pH 6.2), we assembled amyloid fibrils in vitro only from recombinant, ΔN4, ΔN5, and ΔN6 β (2)M but not from WT β (2)M and its three truncated variants ΔN1 to ΔN3. Notably, the removal of the six, five or four residues at the N-terminus leads to enhanced fibril formation, and homo- and heterotypic seeding of ΔN6 fibrils strongly promotes amyloid fibril formation of WT β (2)M and its six truncated variants, including at more physiological pH 6.2. Collectively, these results demonstrated that the residues 4 to 6 at the N-terminus particularly modulate amyloid fibril propagation of β (2)M and the interactions of WT β (2)M with N-terminally truncated variants, potentially indicating the direct relevance to the involvement of the protein’s aggregation in dialysis-related amyloidosis.