Cargando…

Co-infection of Phlebotomus papatasi (Diptera: Psychodidae) gut bacteria with Leishmania major exacerbates the pathological responses of BALB/c mice

Clinical features and severity of the leishmaniasis is extremely intricate and depend on several factors, especially sand fly-derived products. Bacteria in the sand fly’s gut are a perpetual companion of Leishmania parasites. However, consequences of the concomitance of these bacteria and Leishmania...

Descripción completa

Detalles Bibliográficos
Autores principales: Amni, Fariba, Maleki-Ravasan, Naseh, Nateghi-Rostami, Mahmoud, Hadighi, Ramtin, Karimian, Fateh, Meamar, Ahmad Reza, Badirzadeh, Alireza, Parvizi, Parviz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9909354/
https://www.ncbi.nlm.nih.gov/pubmed/36779192
http://dx.doi.org/10.3389/fcimb.2023.1115542
_version_ 1784884556833226752
author Amni, Fariba
Maleki-Ravasan, Naseh
Nateghi-Rostami, Mahmoud
Hadighi, Ramtin
Karimian, Fateh
Meamar, Ahmad Reza
Badirzadeh, Alireza
Parvizi, Parviz
author_facet Amni, Fariba
Maleki-Ravasan, Naseh
Nateghi-Rostami, Mahmoud
Hadighi, Ramtin
Karimian, Fateh
Meamar, Ahmad Reza
Badirzadeh, Alireza
Parvizi, Parviz
author_sort Amni, Fariba
collection PubMed
description Clinical features and severity of the leishmaniasis is extremely intricate and depend on several factors, especially sand fly-derived products. Bacteria in the sand fly’s gut are a perpetual companion of Leishmania parasites. However, consequences of the concomitance of these bacteria and Leishmania parasite outside the midgut environment have not been investigated in the infection process. Herein, a needle infection model was designed to mimic transmission by sand flies, to examine differences in the onset and progression of L. major infection initiated by inoculation with “low” or “high” doses of Enterobacter cloacae and Bacillus subtilis bacteria. The results showed an alteration in the local expression of pro- and anti-inflammatory cytokines in mice receiving different inoculations of bacteria. Simultaneous injection of two bacteria with Leishmania parasites in the low-dose group caused greater thickness of ear pinna and enhanced tissue chronic inflammatory cells, as well as resulted in multifold increase in the expression of IL-4 and IL-1β and a decrease in the iNOS expression, without changing the L. major burden. Despite advances in scientific breakthroughs, scant survey has investigated the interaction between micro and macro levels of organization of leishmaniasis that ranges from the cellular to macro ecosystem levels, giving rise to the spread and persistence of the disease in a region. Our findings provide new insight into using the potential of the vector-derived microbiota in modulating the vertebrate immune system for the benefit of the host or recommend the use of appropriate antibiotics along with antileishmanial medicines.
format Online
Article
Text
id pubmed-9909354
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99093542023-02-10 Co-infection of Phlebotomus papatasi (Diptera: Psychodidae) gut bacteria with Leishmania major exacerbates the pathological responses of BALB/c mice Amni, Fariba Maleki-Ravasan, Naseh Nateghi-Rostami, Mahmoud Hadighi, Ramtin Karimian, Fateh Meamar, Ahmad Reza Badirzadeh, Alireza Parvizi, Parviz Front Cell Infect Microbiol Cellular and Infection Microbiology Clinical features and severity of the leishmaniasis is extremely intricate and depend on several factors, especially sand fly-derived products. Bacteria in the sand fly’s gut are a perpetual companion of Leishmania parasites. However, consequences of the concomitance of these bacteria and Leishmania parasite outside the midgut environment have not been investigated in the infection process. Herein, a needle infection model was designed to mimic transmission by sand flies, to examine differences in the onset and progression of L. major infection initiated by inoculation with “low” or “high” doses of Enterobacter cloacae and Bacillus subtilis bacteria. The results showed an alteration in the local expression of pro- and anti-inflammatory cytokines in mice receiving different inoculations of bacteria. Simultaneous injection of two bacteria with Leishmania parasites in the low-dose group caused greater thickness of ear pinna and enhanced tissue chronic inflammatory cells, as well as resulted in multifold increase in the expression of IL-4 and IL-1β and a decrease in the iNOS expression, without changing the L. major burden. Despite advances in scientific breakthroughs, scant survey has investigated the interaction between micro and macro levels of organization of leishmaniasis that ranges from the cellular to macro ecosystem levels, giving rise to the spread and persistence of the disease in a region. Our findings provide new insight into using the potential of the vector-derived microbiota in modulating the vertebrate immune system for the benefit of the host or recommend the use of appropriate antibiotics along with antileishmanial medicines. Frontiers Media S.A. 2023-01-26 /pmc/articles/PMC9909354/ /pubmed/36779192 http://dx.doi.org/10.3389/fcimb.2023.1115542 Text en Copyright © 2023 Amni, Maleki-Ravasan, Nateghi-Rostami, Hadighi, Karimian, Meamar, Badirzadeh and Parvizi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Amni, Fariba
Maleki-Ravasan, Naseh
Nateghi-Rostami, Mahmoud
Hadighi, Ramtin
Karimian, Fateh
Meamar, Ahmad Reza
Badirzadeh, Alireza
Parvizi, Parviz
Co-infection of Phlebotomus papatasi (Diptera: Psychodidae) gut bacteria with Leishmania major exacerbates the pathological responses of BALB/c mice
title Co-infection of Phlebotomus papatasi (Diptera: Psychodidae) gut bacteria with Leishmania major exacerbates the pathological responses of BALB/c mice
title_full Co-infection of Phlebotomus papatasi (Diptera: Psychodidae) gut bacteria with Leishmania major exacerbates the pathological responses of BALB/c mice
title_fullStr Co-infection of Phlebotomus papatasi (Diptera: Psychodidae) gut bacteria with Leishmania major exacerbates the pathological responses of BALB/c mice
title_full_unstemmed Co-infection of Phlebotomus papatasi (Diptera: Psychodidae) gut bacteria with Leishmania major exacerbates the pathological responses of BALB/c mice
title_short Co-infection of Phlebotomus papatasi (Diptera: Psychodidae) gut bacteria with Leishmania major exacerbates the pathological responses of BALB/c mice
title_sort co-infection of phlebotomus papatasi (diptera: psychodidae) gut bacteria with leishmania major exacerbates the pathological responses of balb/c mice
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9909354/
https://www.ncbi.nlm.nih.gov/pubmed/36779192
http://dx.doi.org/10.3389/fcimb.2023.1115542
work_keys_str_mv AT amnifariba coinfectionofphlebotomuspapatasidipterapsychodidaegutbacteriawithleishmaniamajorexacerbatesthepathologicalresponsesofbalbcmice
AT malekiravasannaseh coinfectionofphlebotomuspapatasidipterapsychodidaegutbacteriawithleishmaniamajorexacerbatesthepathologicalresponsesofbalbcmice
AT nateghirostamimahmoud coinfectionofphlebotomuspapatasidipterapsychodidaegutbacteriawithleishmaniamajorexacerbatesthepathologicalresponsesofbalbcmice
AT hadighiramtin coinfectionofphlebotomuspapatasidipterapsychodidaegutbacteriawithleishmaniamajorexacerbatesthepathologicalresponsesofbalbcmice
AT karimianfateh coinfectionofphlebotomuspapatasidipterapsychodidaegutbacteriawithleishmaniamajorexacerbatesthepathologicalresponsesofbalbcmice
AT meamarahmadreza coinfectionofphlebotomuspapatasidipterapsychodidaegutbacteriawithleishmaniamajorexacerbatesthepathologicalresponsesofbalbcmice
AT badirzadehalireza coinfectionofphlebotomuspapatasidipterapsychodidaegutbacteriawithleishmaniamajorexacerbatesthepathologicalresponsesofbalbcmice
AT parviziparviz coinfectionofphlebotomuspapatasidipterapsychodidaegutbacteriawithleishmaniamajorexacerbatesthepathologicalresponsesofbalbcmice