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Screening Multidrug Resistance Reversal Agents in Traditional Chinese Medicines by Efflux Kinetics of D-Luciferin in MCF-7/DOX(Fluc) Cells
[Image: see text] In this study, we established a simple and rapid in vitro method for screening multidrug resistance (MDR) reversal agents in traditional Chinese medicines (TCMs), which could better correspond to the MDR reversing effect in vivo. Here, D-luciferin, a substrate for the enzyme firefl...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9909823/ https://www.ncbi.nlm.nih.gov/pubmed/36777569 http://dx.doi.org/10.1021/acsomega.2c07096 |
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author | Zhao, Yue Tang, Chaoyuan Huang, Jingyi Zhang, Hongyan Shi, Jingbin Xu, Shujun Ma, Lisha Peng, Chun Liu, Qi Xiong, Yang |
author_facet | Zhao, Yue Tang, Chaoyuan Huang, Jingyi Zhang, Hongyan Shi, Jingbin Xu, Shujun Ma, Lisha Peng, Chun Liu, Qi Xiong, Yang |
author_sort | Zhao, Yue |
collection | PubMed |
description | [Image: see text] In this study, we established a simple and rapid in vitro method for screening multidrug resistance (MDR) reversal agents in traditional Chinese medicines (TCMs), which could better correspond to the MDR reversing effect in vivo. Here, D-luciferin, a substrate for the enzyme firefly luciferase and also a substrate for ATP-binding cassette transporters (ABC transporters), was used as the probe to detect its efflux kinetics caused by ABC transporters. First, we established a stable doxorubicin (DOX)-resistant cell line (MCF-7/DOX(Fluc)) that overexpressed luciferase. Then, some kinds of TCMs were chosen for the MDR reversal agents to measure its effect on inhibiting the D-luciferin outflow from MCF-7/DOX(Fluc), and the ideal reversal agent with the least D-luciferin efflux from MCF-7/DOX(Fluc) was selected to further investigate its effect combined with DOX on MCF-7/DOX(Fluc) tumor-bearing mice. The results indicated that quercetin (Qu) could remarkably increase the retention of D-luciferin in MCF-7/DOX(Fluc)in vitro and in vivo. Also, the combination of Qu and DOX could exceedingly inhibit the tumor growth, which proved the feasibility of this in vitro screening method. The study proposed a feasible method for mass screening of MDR agents from TCMs in vitro. |
format | Online Article Text |
id | pubmed-9909823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-99098232023-02-10 Screening Multidrug Resistance Reversal Agents in Traditional Chinese Medicines by Efflux Kinetics of D-Luciferin in MCF-7/DOX(Fluc) Cells Zhao, Yue Tang, Chaoyuan Huang, Jingyi Zhang, Hongyan Shi, Jingbin Xu, Shujun Ma, Lisha Peng, Chun Liu, Qi Xiong, Yang ACS Omega [Image: see text] In this study, we established a simple and rapid in vitro method for screening multidrug resistance (MDR) reversal agents in traditional Chinese medicines (TCMs), which could better correspond to the MDR reversing effect in vivo. Here, D-luciferin, a substrate for the enzyme firefly luciferase and also a substrate for ATP-binding cassette transporters (ABC transporters), was used as the probe to detect its efflux kinetics caused by ABC transporters. First, we established a stable doxorubicin (DOX)-resistant cell line (MCF-7/DOX(Fluc)) that overexpressed luciferase. Then, some kinds of TCMs were chosen for the MDR reversal agents to measure its effect on inhibiting the D-luciferin outflow from MCF-7/DOX(Fluc), and the ideal reversal agent with the least D-luciferin efflux from MCF-7/DOX(Fluc) was selected to further investigate its effect combined with DOX on MCF-7/DOX(Fluc) tumor-bearing mice. The results indicated that quercetin (Qu) could remarkably increase the retention of D-luciferin in MCF-7/DOX(Fluc)in vitro and in vivo. Also, the combination of Qu and DOX could exceedingly inhibit the tumor growth, which proved the feasibility of this in vitro screening method. The study proposed a feasible method for mass screening of MDR agents from TCMs in vitro. American Chemical Society 2023-01-23 /pmc/articles/PMC9909823/ /pubmed/36777569 http://dx.doi.org/10.1021/acsomega.2c07096 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Zhao, Yue Tang, Chaoyuan Huang, Jingyi Zhang, Hongyan Shi, Jingbin Xu, Shujun Ma, Lisha Peng, Chun Liu, Qi Xiong, Yang Screening Multidrug Resistance Reversal Agents in Traditional Chinese Medicines by Efflux Kinetics of D-Luciferin in MCF-7/DOX(Fluc) Cells |
title | Screening Multidrug
Resistance Reversal Agents in
Traditional Chinese Medicines by Efflux Kinetics of D-Luciferin
in MCF-7/DOX(Fluc) Cells |
title_full | Screening Multidrug
Resistance Reversal Agents in
Traditional Chinese Medicines by Efflux Kinetics of D-Luciferin
in MCF-7/DOX(Fluc) Cells |
title_fullStr | Screening Multidrug
Resistance Reversal Agents in
Traditional Chinese Medicines by Efflux Kinetics of D-Luciferin
in MCF-7/DOX(Fluc) Cells |
title_full_unstemmed | Screening Multidrug
Resistance Reversal Agents in
Traditional Chinese Medicines by Efflux Kinetics of D-Luciferin
in MCF-7/DOX(Fluc) Cells |
title_short | Screening Multidrug
Resistance Reversal Agents in
Traditional Chinese Medicines by Efflux Kinetics of D-Luciferin
in MCF-7/DOX(Fluc) Cells |
title_sort | screening multidrug
resistance reversal agents in
traditional chinese medicines by efflux kinetics of d-luciferin
in mcf-7/dox(fluc) cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9909823/ https://www.ncbi.nlm.nih.gov/pubmed/36777569 http://dx.doi.org/10.1021/acsomega.2c07096 |
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