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Heterogeneous effects of individual high-fat diet compositions on phenotype, metabolic outcome, and hepatic proteome signature in BL/6 male mice

The multitude of obesogenic diets used in rodent studies can hardly be overviewed. Since standardization is missing and assuming that individual compositions provoke individual effects, the choice of quality, quantity and combination of diet ingredients seems to be crucial for the outcome and interp...

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Detalles Bibliográficos
Autores principales: Janoschek, Ruth, Handwerk, Marion, Hucklenbruch-Rother, Eva, Schmitz, Lisa, Bae-Gartz, Inga, Kasper, Philipp, Lackmann, Jan-Wilm, Kretschmer, Tobias, Vohlen, Christina, Mesaros, Andrea, Purrio, Martin, Quaas, Alexander, Dötsch, Jörg, Appel, Sarah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9909936/
https://www.ncbi.nlm.nih.gov/pubmed/36755289
http://dx.doi.org/10.1186/s12986-023-00729-0
Descripción
Sumario:The multitude of obesogenic diets used in rodent studies can hardly be overviewed. Since standardization is missing and assuming that individual compositions provoke individual effects, the choice of quality, quantity and combination of diet ingredients seems to be crucial for the outcome and interpretation of obesity studies. Therefore, the present study was conducted to compare the individual effects of three commonly used obesogenic diets, mainly differing in sugar and fat content. Besides basic phenotypic and metabolic characterization, one main aspect was a comparative liver proteome analysis. As expected, the obtained results picture differentiated consequences mainly depending on fat source and/or fat- and sugar quantity. By confirming the general presumption that the choice of nutritional composition is a pivotal factor, the present findings demonstrate that a conscious selection is indispensable for obtaining reliable and sound results in obesity research. In conclusion, we strongly recommend a careful selection of the appropriate diet in advance of a new experiment, taking into account the specific research question. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-023-00729-0.