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B-cell ELISpot assay to analyze human memory B cell and plasmablast responses specific to SARS-CoV-2 receptor-binding domain

B-cell ELISpot is an extremely sensitive assay based on the secretion of antibodies by B cells that requires the differentiation of B cells into antibody-secreting cells. Here, we describe the procedure to analyze both plasmablast (PB) and memory B cell (MBC) responses specific to SARS-CoV-2 recepto...

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Detalles Bibliográficos
Autores principales:	Rouers, Angeline, Tay, Matthew Zirui, Ng, Lisa F.P., Renia, Laurent
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910018/ https://www.ncbi.nlm.nih.gov/pubmed/36853725 http://dx.doi.org/10.1016/j.xpro.2023.102130

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