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Fabrication and In Vitro Drug Delivery Evaluation of Cephalexin Monohydrate-Loaded PLA:PVA/HAP:TiO(2) Fibrous Scaffolds for Bone Regeneration

[Image: see text] Owing to the excellent osteoconductive property of hydroxyapatite, we aimed to design a cephalexin monohydrate-loaded PLA:PVA/HAP:TiO(2) nanofibrous scaffold to improve the drug delivery efficiency toward bone regenerative applications. In this study, HAP:TiO(2) (anatase and rutile...

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Autores principales: Murugapandian, Rama, Clement, Simona, Uthirapathy, Vijayalakshmi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910077/
https://www.ncbi.nlm.nih.gov/pubmed/36777593
http://dx.doi.org/10.1021/acsomega.2c07701
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author Murugapandian, Rama
Clement, Simona
Uthirapathy, Vijayalakshmi
author_facet Murugapandian, Rama
Clement, Simona
Uthirapathy, Vijayalakshmi
author_sort Murugapandian, Rama
collection PubMed
description [Image: see text] Owing to the excellent osteoconductive property of hydroxyapatite, we aimed to design a cephalexin monohydrate-loaded PLA:PVA/HAP:TiO(2) nanofibrous scaffold to improve the drug delivery efficiency toward bone regenerative applications. In this study, HAP:TiO(2) (anatase and rutile phases) samples were prepared by a coprecipitation method, which were later blended with PLA:PVA polymeric solution (with and without the drug) to fabricate a nanofibrous matrix via the electrospinning technique. All the prepared samples were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, contact angle, porosity, and tensile strength tests. Further, in vitro biodegradation and the drug-releasing ability were examined by varying the concentration of cephalexin monohydrate in the composite matrix. Deposition of the apatite layer on the scaffolds was examined after incubation in simulated body fluid solution to confirm the bioactivity of the prepared nanofibers. Biocompatibility by the MTT assay and osteogenic differentiation by ARS staining were evaluated by culturing MG63 cells on PLA:PVA/HAP:TiO(2) nanofibers, which could ensue better support for cell proliferation. Consequently, the sustained release profile and better biocompatibility of the scaffolds revealed a strong potential use in bone regenerative applications.
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spelling pubmed-99100772023-02-10 Fabrication and In Vitro Drug Delivery Evaluation of Cephalexin Monohydrate-Loaded PLA:PVA/HAP:TiO(2) Fibrous Scaffolds for Bone Regeneration Murugapandian, Rama Clement, Simona Uthirapathy, Vijayalakshmi ACS Omega [Image: see text] Owing to the excellent osteoconductive property of hydroxyapatite, we aimed to design a cephalexin monohydrate-loaded PLA:PVA/HAP:TiO(2) nanofibrous scaffold to improve the drug delivery efficiency toward bone regenerative applications. In this study, HAP:TiO(2) (anatase and rutile phases) samples were prepared by a coprecipitation method, which were later blended with PLA:PVA polymeric solution (with and without the drug) to fabricate a nanofibrous matrix via the electrospinning technique. All the prepared samples were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, contact angle, porosity, and tensile strength tests. Further, in vitro biodegradation and the drug-releasing ability were examined by varying the concentration of cephalexin monohydrate in the composite matrix. Deposition of the apatite layer on the scaffolds was examined after incubation in simulated body fluid solution to confirm the bioactivity of the prepared nanofibers. Biocompatibility by the MTT assay and osteogenic differentiation by ARS staining were evaluated by culturing MG63 cells on PLA:PVA/HAP:TiO(2) nanofibers, which could ensue better support for cell proliferation. Consequently, the sustained release profile and better biocompatibility of the scaffolds revealed a strong potential use in bone regenerative applications. American Chemical Society 2023-01-23 /pmc/articles/PMC9910077/ /pubmed/36777593 http://dx.doi.org/10.1021/acsomega.2c07701 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Murugapandian, Rama
Clement, Simona
Uthirapathy, Vijayalakshmi
Fabrication and In Vitro Drug Delivery Evaluation of Cephalexin Monohydrate-Loaded PLA:PVA/HAP:TiO(2) Fibrous Scaffolds for Bone Regeneration
title Fabrication and In Vitro Drug Delivery Evaluation of Cephalexin Monohydrate-Loaded PLA:PVA/HAP:TiO(2) Fibrous Scaffolds for Bone Regeneration
title_full Fabrication and In Vitro Drug Delivery Evaluation of Cephalexin Monohydrate-Loaded PLA:PVA/HAP:TiO(2) Fibrous Scaffolds for Bone Regeneration
title_fullStr Fabrication and In Vitro Drug Delivery Evaluation of Cephalexin Monohydrate-Loaded PLA:PVA/HAP:TiO(2) Fibrous Scaffolds for Bone Regeneration
title_full_unstemmed Fabrication and In Vitro Drug Delivery Evaluation of Cephalexin Monohydrate-Loaded PLA:PVA/HAP:TiO(2) Fibrous Scaffolds for Bone Regeneration
title_short Fabrication and In Vitro Drug Delivery Evaluation of Cephalexin Monohydrate-Loaded PLA:PVA/HAP:TiO(2) Fibrous Scaffolds for Bone Regeneration
title_sort fabrication and in vitro drug delivery evaluation of cephalexin monohydrate-loaded pla:pva/hap:tio(2) fibrous scaffolds for bone regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910077/
https://www.ncbi.nlm.nih.gov/pubmed/36777593
http://dx.doi.org/10.1021/acsomega.2c07701
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