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Identification of sugar transporter genes and their roles in the pathogenicity of Verticillium dahliae on cotton

INTRODUCTION: Verticillium wilt (VW) caused by Verticillium dahliae is a soil-borne vascular fungal disease that severely affects cotton yield and fiber quality. Sugar metabolism plays an important role in the growth and pathogenicity of V. dahliae. However, limited information is known about the su...

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Autores principales: Chen, Lihua, Chen, Bin, Zhu, Qian-Hao, Zhang, Xinyu, Sun, Tiange, Liu, Feng, Yang, Yonglin, Sun, Jie, Li, Yanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910176/
https://www.ncbi.nlm.nih.gov/pubmed/36778686
http://dx.doi.org/10.3389/fpls.2023.1123523
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author Chen, Lihua
Chen, Bin
Zhu, Qian-Hao
Zhang, Xinyu
Sun, Tiange
Liu, Feng
Yang, Yonglin
Sun, Jie
Li, Yanjun
author_facet Chen, Lihua
Chen, Bin
Zhu, Qian-Hao
Zhang, Xinyu
Sun, Tiange
Liu, Feng
Yang, Yonglin
Sun, Jie
Li, Yanjun
author_sort Chen, Lihua
collection PubMed
description INTRODUCTION: Verticillium wilt (VW) caused by Verticillium dahliae is a soil-borne vascular fungal disease that severely affects cotton yield and fiber quality. Sugar metabolism plays an important role in the growth and pathogenicity of V. dahliae. However, limited information is known about the sugar transporter genes and their roles in the growth and pathogenicity of V. dahliae. METHOD: In this study, genome-wide identification of sugar transporter genes in V. dahliae was conducted and the expression profiles of these genes in response to root exudates from cotton varieties susceptible or resistant to V. dahliae were investigated based on RNA-seq data. Tobacco Rattle Virus-based host-induced gene silencing (TRV-based HIGS) and artificial small interfering RNAs (asiRNAs) were applied to investigate the function of candidate genes involved in the growth and pathogenic process of V. dahliae. RESULTS: A total of 65 putative sugar transporter genes were identified and clustered into 8 Clades. Of the 65 sugar transporter genes, 9 were found to be induced only by root exudates from the susceptible variety, including VdST3 and VdST12 that were selected for further functional study. Silencing of VdST3 or VdST12 in host plants by TRV-based HIGS reduced fungal biomass and enhanced cotton resistance against V. dahliae. Additionally, silencing of VdST12 and VdST3 by feeding asiRNAs targeting VdST12 (asiR815 or asiR1436) and VdST3 (asiR201 or asiR1238) inhibited fungal growth, exhibiting significant reduction in hyphae and colony diameter, with a more significant effect observed for the asiRNAs targeting VdST12. The inhibitory effect of asiRNAs on the growth of V. dahliae was enhanced with the increasing concentration of asiRNAs. Silencing of VdST12 by feeding asiR815+asiR1436 significantly decreased the pathogenicity of V. dahliae. DISCUSSION: The results suggest that VdST3 and VdST12 are sugar transporter genes required for growth and pathogenicity of V. dahliae and that asiRNA is a valuable tool for functional characterization of V. dahliae genes.
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spelling pubmed-99101762023-02-10 Identification of sugar transporter genes and their roles in the pathogenicity of Verticillium dahliae on cotton Chen, Lihua Chen, Bin Zhu, Qian-Hao Zhang, Xinyu Sun, Tiange Liu, Feng Yang, Yonglin Sun, Jie Li, Yanjun Front Plant Sci Plant Science INTRODUCTION: Verticillium wilt (VW) caused by Verticillium dahliae is a soil-borne vascular fungal disease that severely affects cotton yield and fiber quality. Sugar metabolism plays an important role in the growth and pathogenicity of V. dahliae. However, limited information is known about the sugar transporter genes and their roles in the growth and pathogenicity of V. dahliae. METHOD: In this study, genome-wide identification of sugar transporter genes in V. dahliae was conducted and the expression profiles of these genes in response to root exudates from cotton varieties susceptible or resistant to V. dahliae were investigated based on RNA-seq data. Tobacco Rattle Virus-based host-induced gene silencing (TRV-based HIGS) and artificial small interfering RNAs (asiRNAs) were applied to investigate the function of candidate genes involved in the growth and pathogenic process of V. dahliae. RESULTS: A total of 65 putative sugar transporter genes were identified and clustered into 8 Clades. Of the 65 sugar transporter genes, 9 were found to be induced only by root exudates from the susceptible variety, including VdST3 and VdST12 that were selected for further functional study. Silencing of VdST3 or VdST12 in host plants by TRV-based HIGS reduced fungal biomass and enhanced cotton resistance against V. dahliae. Additionally, silencing of VdST12 and VdST3 by feeding asiRNAs targeting VdST12 (asiR815 or asiR1436) and VdST3 (asiR201 or asiR1238) inhibited fungal growth, exhibiting significant reduction in hyphae and colony diameter, with a more significant effect observed for the asiRNAs targeting VdST12. The inhibitory effect of asiRNAs on the growth of V. dahliae was enhanced with the increasing concentration of asiRNAs. Silencing of VdST12 by feeding asiR815+asiR1436 significantly decreased the pathogenicity of V. dahliae. DISCUSSION: The results suggest that VdST3 and VdST12 are sugar transporter genes required for growth and pathogenicity of V. dahliae and that asiRNA is a valuable tool for functional characterization of V. dahliae genes. Frontiers Media S.A. 2023-01-26 /pmc/articles/PMC9910176/ /pubmed/36778686 http://dx.doi.org/10.3389/fpls.2023.1123523 Text en Copyright © 2023 Chen, Chen, Zhu, Zhang, Sun, Liu, Yang, Sun and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chen, Lihua
Chen, Bin
Zhu, Qian-Hao
Zhang, Xinyu
Sun, Tiange
Liu, Feng
Yang, Yonglin
Sun, Jie
Li, Yanjun
Identification of sugar transporter genes and their roles in the pathogenicity of Verticillium dahliae on cotton
title Identification of sugar transporter genes and their roles in the pathogenicity of Verticillium dahliae on cotton
title_full Identification of sugar transporter genes and their roles in the pathogenicity of Verticillium dahliae on cotton
title_fullStr Identification of sugar transporter genes and their roles in the pathogenicity of Verticillium dahliae on cotton
title_full_unstemmed Identification of sugar transporter genes and their roles in the pathogenicity of Verticillium dahliae on cotton
title_short Identification of sugar transporter genes and their roles in the pathogenicity of Verticillium dahliae on cotton
title_sort identification of sugar transporter genes and their roles in the pathogenicity of verticillium dahliae on cotton
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910176/
https://www.ncbi.nlm.nih.gov/pubmed/36778686
http://dx.doi.org/10.3389/fpls.2023.1123523
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