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Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients

Human induced pluripotent stem cells (iPSCs) provide a virtually inexhaustible source of starting material for next generation cell therapies, offering new opportunities for regenerative medicine. Among different cell sources for the generation of iPSCs, urine cells are clinically relevant since the...

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Autores principales: Baliña-Sánchez, Carmen, Aguilera, Yolanda, Adán, Norma, Sierra-Párraga, Jesús María, Olmedo-Moreno, Laura, Panadero-Morón, Concepción, Cabello-Laureano, Rosa, Márquez-Vega, Catalina, Martín-Montalvo, Alejandro, Capilla-González, Vivian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910217/
https://www.ncbi.nlm.nih.gov/pubmed/36776860
http://dx.doi.org/10.3389/fimmu.2023.1022676
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author Baliña-Sánchez, Carmen
Aguilera, Yolanda
Adán, Norma
Sierra-Párraga, Jesús María
Olmedo-Moreno, Laura
Panadero-Morón, Concepción
Cabello-Laureano, Rosa
Márquez-Vega, Catalina
Martín-Montalvo, Alejandro
Capilla-González, Vivian
author_facet Baliña-Sánchez, Carmen
Aguilera, Yolanda
Adán, Norma
Sierra-Párraga, Jesús María
Olmedo-Moreno, Laura
Panadero-Morón, Concepción
Cabello-Laureano, Rosa
Márquez-Vega, Catalina
Martín-Montalvo, Alejandro
Capilla-González, Vivian
author_sort Baliña-Sánchez, Carmen
collection PubMed
description Human induced pluripotent stem cells (iPSCs) provide a virtually inexhaustible source of starting material for next generation cell therapies, offering new opportunities for regenerative medicine. Among different cell sources for the generation of iPSCs, urine cells are clinically relevant since these cells can be repeatedly obtained by non-invasive methods from patients of any age and health condition. These attributes encourage patients to participate in preclinical and clinical research. In particular, the use of urine-derived iPSC products is a convenient strategy for children with brain tumors, which are medically fragile patients. Here, we investigate the feasibility of using urine samples as a source of somatic cells to generate iPSC lines from pediatric patients with brain tumors (BT-iPSC). Urinary epithelial cells were isolated and reprogrammed using non-integrative Sendai virus vectors harboring the Yamanaka factors KLF4, OCT3/4, SOX2 and C-MYC. After reprogramming, BT-iPSC lines were subject to quality assessment and were compared to iPSCs obtained from urine samples of non-tumor pediatric patients (nonT-iPSC). We demonstrated that iPSCs can be successfully derived from a small volume of urine obtained from pediatric patients. Importantly, we showed that BT-iPSCs are equivalent to nonT-iPSCs in terms of morphology, pluripotency, and differentiation capacity into the three germ layers. In addition, both BT-iPSCs and nonT-iPSCs efficiently differentiated into functional mesenchymal stem/stromal cells (iMSC) with immunomodulatory properties. Therefore, this study provides an attractive approach to non-invasively generate personalized iMSC products intended for the treatment of children with brain tumors.
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spelling pubmed-99102172023-02-10 Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients Baliña-Sánchez, Carmen Aguilera, Yolanda Adán, Norma Sierra-Párraga, Jesús María Olmedo-Moreno, Laura Panadero-Morón, Concepción Cabello-Laureano, Rosa Márquez-Vega, Catalina Martín-Montalvo, Alejandro Capilla-González, Vivian Front Immunol Immunology Human induced pluripotent stem cells (iPSCs) provide a virtually inexhaustible source of starting material for next generation cell therapies, offering new opportunities for regenerative medicine. Among different cell sources for the generation of iPSCs, urine cells are clinically relevant since these cells can be repeatedly obtained by non-invasive methods from patients of any age and health condition. These attributes encourage patients to participate in preclinical and clinical research. In particular, the use of urine-derived iPSC products is a convenient strategy for children with brain tumors, which are medically fragile patients. Here, we investigate the feasibility of using urine samples as a source of somatic cells to generate iPSC lines from pediatric patients with brain tumors (BT-iPSC). Urinary epithelial cells were isolated and reprogrammed using non-integrative Sendai virus vectors harboring the Yamanaka factors KLF4, OCT3/4, SOX2 and C-MYC. After reprogramming, BT-iPSC lines were subject to quality assessment and were compared to iPSCs obtained from urine samples of non-tumor pediatric patients (nonT-iPSC). We demonstrated that iPSCs can be successfully derived from a small volume of urine obtained from pediatric patients. Importantly, we showed that BT-iPSCs are equivalent to nonT-iPSCs in terms of morphology, pluripotency, and differentiation capacity into the three germ layers. In addition, both BT-iPSCs and nonT-iPSCs efficiently differentiated into functional mesenchymal stem/stromal cells (iMSC) with immunomodulatory properties. Therefore, this study provides an attractive approach to non-invasively generate personalized iMSC products intended for the treatment of children with brain tumors. Frontiers Media S.A. 2023-01-26 /pmc/articles/PMC9910217/ /pubmed/36776860 http://dx.doi.org/10.3389/fimmu.2023.1022676 Text en Copyright © 2023 Baliña-Sánchez, Aguilera, Adán, Sierra-Párraga, Olmedo-Moreno, Panadero-Morón, Cabello-Laureano, Márquez-Vega, Martín-Montalvo and Capilla-González https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Baliña-Sánchez, Carmen
Aguilera, Yolanda
Adán, Norma
Sierra-Párraga, Jesús María
Olmedo-Moreno, Laura
Panadero-Morón, Concepción
Cabello-Laureano, Rosa
Márquez-Vega, Catalina
Martín-Montalvo, Alejandro
Capilla-González, Vivian
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients
title Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients
title_full Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients
title_fullStr Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients
title_full_unstemmed Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients
title_short Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients
title_sort generation of mesenchymal stromal cells from urine-derived ipscs of pediatric brain tumor patients
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910217/
https://www.ncbi.nlm.nih.gov/pubmed/36776860
http://dx.doi.org/10.3389/fimmu.2023.1022676
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