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Recombinant fiber-1 protein of fowl adenovirus serotype 4 induces high levels of neutralizing antibodies in immunized chickens

Virulent fowl adenovirus serotype 4 (FAdV-4) causes hydropericardium syndrome (HPS) with high mortality in chickens, leading to significant economic losses to the poultry industry. The development of an effective vaccine is essential for successful disease control. Here, we produced recombinant fibe...

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Autores principales: Watanabe, Satoko, Yamamoto, Yu, Kurokawa, Aoi, Iseki, Hiroshi, Tanikawa, Taichiro, Mase, Masaji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910269/
https://www.ncbi.nlm.nih.gov/pubmed/36757474
http://dx.doi.org/10.1007/s00705-023-05709-6
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author Watanabe, Satoko
Yamamoto, Yu
Kurokawa, Aoi
Iseki, Hiroshi
Tanikawa, Taichiro
Mase, Masaji
author_facet Watanabe, Satoko
Yamamoto, Yu
Kurokawa, Aoi
Iseki, Hiroshi
Tanikawa, Taichiro
Mase, Masaji
author_sort Watanabe, Satoko
collection PubMed
description Virulent fowl adenovirus serotype 4 (FAdV-4) causes hydropericardium syndrome (HPS) with high mortality in chickens, leading to significant economic losses to the poultry industry. The development of an effective vaccine is essential for successful disease control. Here, we produced recombinant fiber-1 protein of FAdV-4, isolated from a Japanese HPS outbreak strain, JP/LVP-1/96, using a baculovirus expression system and evaluated its immunogenicity and protective efficacy. Recombinant fiber-1 protein induced high levels of neutralizing antibodies in immunized chickens, which were maintained for a minimum of 10 weeks. After being challenged with the virulent FAdV-4 strain JP/LVP-1/96, the immunized chickens did not exhibit clinical signs of infection or histopathological changes, there was a significant reduction in the viral load in various organs and total serum proteins, and albumin levels did not decline. These results suggest that the recombinant fiber-1 protein produced in this study can serve as a subunit vaccine to control HPS in chickens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05709-6.
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spelling pubmed-99102692023-02-10 Recombinant fiber-1 protein of fowl adenovirus serotype 4 induces high levels of neutralizing antibodies in immunized chickens Watanabe, Satoko Yamamoto, Yu Kurokawa, Aoi Iseki, Hiroshi Tanikawa, Taichiro Mase, Masaji Arch Virol Original Article Virulent fowl adenovirus serotype 4 (FAdV-4) causes hydropericardium syndrome (HPS) with high mortality in chickens, leading to significant economic losses to the poultry industry. The development of an effective vaccine is essential for successful disease control. Here, we produced recombinant fiber-1 protein of FAdV-4, isolated from a Japanese HPS outbreak strain, JP/LVP-1/96, using a baculovirus expression system and evaluated its immunogenicity and protective efficacy. Recombinant fiber-1 protein induced high levels of neutralizing antibodies in immunized chickens, which were maintained for a minimum of 10 weeks. After being challenged with the virulent FAdV-4 strain JP/LVP-1/96, the immunized chickens did not exhibit clinical signs of infection or histopathological changes, there was a significant reduction in the viral load in various organs and total serum proteins, and albumin levels did not decline. These results suggest that the recombinant fiber-1 protein produced in this study can serve as a subunit vaccine to control HPS in chickens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05709-6. Springer Vienna 2023-02-09 2023 /pmc/articles/PMC9910269/ /pubmed/36757474 http://dx.doi.org/10.1007/s00705-023-05709-6 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Watanabe, Satoko
Yamamoto, Yu
Kurokawa, Aoi
Iseki, Hiroshi
Tanikawa, Taichiro
Mase, Masaji
Recombinant fiber-1 protein of fowl adenovirus serotype 4 induces high levels of neutralizing antibodies in immunized chickens
title Recombinant fiber-1 protein of fowl adenovirus serotype 4 induces high levels of neutralizing antibodies in immunized chickens
title_full Recombinant fiber-1 protein of fowl adenovirus serotype 4 induces high levels of neutralizing antibodies in immunized chickens
title_fullStr Recombinant fiber-1 protein of fowl adenovirus serotype 4 induces high levels of neutralizing antibodies in immunized chickens
title_full_unstemmed Recombinant fiber-1 protein of fowl adenovirus serotype 4 induces high levels of neutralizing antibodies in immunized chickens
title_short Recombinant fiber-1 protein of fowl adenovirus serotype 4 induces high levels of neutralizing antibodies in immunized chickens
title_sort recombinant fiber-1 protein of fowl adenovirus serotype 4 induces high levels of neutralizing antibodies in immunized chickens
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910269/
https://www.ncbi.nlm.nih.gov/pubmed/36757474
http://dx.doi.org/10.1007/s00705-023-05709-6
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