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Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications

BACKGROUND: Chronic hyperglycaemia of diabetes causes long-term damage and impaired function of multiple organs. However, the pathological changes in the liver following long-term diabetes remain unclear. This study aimed to determine the pathological complications of long-term diabetes in the rat l...

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Autores principales: Yuniartha, Ratih, Arfian, Nur, Setyaningsih, Wiwit Ananda Wahyu, Kencana, Sagita Mega Sekar, Sari, Dwi Cahyani Ratna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Penerbit Universiti Sains Malaysia 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910368/
https://www.ncbi.nlm.nih.gov/pubmed/36818894
http://dx.doi.org/10.21315/mjms2022.29.6.5
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author Yuniartha, Ratih
Arfian, Nur
Setyaningsih, Wiwit Ananda Wahyu
Kencana, Sagita Mega Sekar
Sari, Dwi Cahyani Ratna
author_facet Yuniartha, Ratih
Arfian, Nur
Setyaningsih, Wiwit Ananda Wahyu
Kencana, Sagita Mega Sekar
Sari, Dwi Cahyani Ratna
author_sort Yuniartha, Ratih
collection PubMed
description BACKGROUND: Chronic hyperglycaemia of diabetes causes long-term damage and impaired function of multiple organs. However, the pathological changes in the liver following long-term diabetes remain unclear. This study aimed to determine the pathological complications of long-term diabetes in the rat liver. METHODS: Intraperitoneal injection of streptozotocin (STZ) was used to induce diabetes in rats at a single dose (60 mg/kg body weight [BW]). Rats were euthanised at 1 month (DM1 group), 2 months (DM2 group) and 4 months (DM4 group) following diabetes induction with six rats in each group. Immunohistochemistry was performed against SOD1, CD68, p53 and p16 antibodies. Messenger RNA (mRNA) expressions of SOD1, SOD2, GPx, CD68, p53, p21 and caspase-3 genes were measured by reverse transcription-polymerase chain reaction. RESULTS: Hepatic p53 mRNA expression was significantly higher in DM1, DM2 and DM4 groups compared to the control group. The p21 and caspase-3 mRNA expressions were significantly upregulated in the DM2 and DM4 groups. The p16-positive cells were obviously increased, particularly in the DM4 group. Bivariate correlation analysis showed mRNA expressions of p21 and caspase-3 genes were positively correlated with the p53 gene. CONCLUSION: Diabetic rats exhibited increased apoptosis and senescence in the liver following a longer period of hyperglycaemia.
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spelling pubmed-99103682023-02-16 Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications Yuniartha, Ratih Arfian, Nur Setyaningsih, Wiwit Ananda Wahyu Kencana, Sagita Mega Sekar Sari, Dwi Cahyani Ratna Malays J Med Sci Original Article BACKGROUND: Chronic hyperglycaemia of diabetes causes long-term damage and impaired function of multiple organs. However, the pathological changes in the liver following long-term diabetes remain unclear. This study aimed to determine the pathological complications of long-term diabetes in the rat liver. METHODS: Intraperitoneal injection of streptozotocin (STZ) was used to induce diabetes in rats at a single dose (60 mg/kg body weight [BW]). Rats were euthanised at 1 month (DM1 group), 2 months (DM2 group) and 4 months (DM4 group) following diabetes induction with six rats in each group. Immunohistochemistry was performed against SOD1, CD68, p53 and p16 antibodies. Messenger RNA (mRNA) expressions of SOD1, SOD2, GPx, CD68, p53, p21 and caspase-3 genes were measured by reverse transcription-polymerase chain reaction. RESULTS: Hepatic p53 mRNA expression was significantly higher in DM1, DM2 and DM4 groups compared to the control group. The p21 and caspase-3 mRNA expressions were significantly upregulated in the DM2 and DM4 groups. The p16-positive cells were obviously increased, particularly in the DM4 group. Bivariate correlation analysis showed mRNA expressions of p21 and caspase-3 genes were positively correlated with the p53 gene. CONCLUSION: Diabetic rats exhibited increased apoptosis and senescence in the liver following a longer period of hyperglycaemia. Penerbit Universiti Sains Malaysia 2022-12 2022-12-22 /pmc/articles/PMC9910368/ /pubmed/36818894 http://dx.doi.org/10.21315/mjms2022.29.6.5 Text en © Penerbit Universiti Sains Malaysia, 2022 https://creativecommons.org/licenses/by/4.0/This work is licensed under the terms of the Creative Commons Attribution (CC BY) (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Original Article
Yuniartha, Ratih
Arfian, Nur
Setyaningsih, Wiwit Ananda Wahyu
Kencana, Sagita Mega Sekar
Sari, Dwi Cahyani Ratna
Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications
title Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications
title_full Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications
title_fullStr Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications
title_full_unstemmed Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications
title_short Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications
title_sort accelerated senescence and apoptosis in the rat liver during the progression of diabetic complications
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910368/
https://www.ncbi.nlm.nih.gov/pubmed/36818894
http://dx.doi.org/10.21315/mjms2022.29.6.5
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