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Photothrombotic Middle Cerebral Artery Occlusion in Mice: A Novel Model of Ischemic Stroke
Stroke is one of the main causes of death and disability worldwide. Over the past decades, several animal models of focal cerebral ischemia have been developed allowing to investigate pathophysiological mechanisms underlying stroke progression. Despite intense preclinical research efforts, the need...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910575/ https://www.ncbi.nlm.nih.gov/pubmed/36650068 http://dx.doi.org/10.1523/ENEURO.0244-22.2022 |
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author | Conti, Emilia Carlini, Noemi Piccardi, Benedetta Allegra Mascaro, Anna Letizia Pavone, Francesco Saverio |
author_facet | Conti, Emilia Carlini, Noemi Piccardi, Benedetta Allegra Mascaro, Anna Letizia Pavone, Francesco Saverio |
author_sort | Conti, Emilia |
collection | PubMed |
description | Stroke is one of the main causes of death and disability worldwide. Over the past decades, several animal models of focal cerebral ischemia have been developed allowing to investigate pathophysiological mechanisms underlying stroke progression. Despite intense preclinical research efforts, the need for noninvasive mouse models of vascular occlusion targeting the middle cerebral artery yet avoiding mechanical intervention is still pressing. Here, by applying the photothrombotic stroke model to the distal branch of the middle cerebral artery, we developed a novel strategy to induce a targeted occlusion of a large blood vessel in mice. This approach induces unilateral damage encompassing most of the dorsal cortex from the motor up to the visual regions 1 week after stroke. Pronounced limb dystonia one day after the damage is partially recovered after one week. Furthermore, we observe the insurgence of blood vessel leakage and edema formation in the peri-infarct area. Finally, this model elicits a notable inflammatory response revealed as a strong increase in astrocyte density and morphologic complexity in the perilesional region of the cortex compared with both other regions of the ipsilesional and contralesional hemispheres, and in sham-operated mice. To conclude, the stroke model we developed induces in mice the light-mediated occlusion of one of the main targets of human ischemic stroke, the middle cerebral artery, free from the limitations of commonly used preclinical models. |
format | Online Article Text |
id | pubmed-9910575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Society for Neuroscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-99105752023-02-10 Photothrombotic Middle Cerebral Artery Occlusion in Mice: A Novel Model of Ischemic Stroke Conti, Emilia Carlini, Noemi Piccardi, Benedetta Allegra Mascaro, Anna Letizia Pavone, Francesco Saverio eNeuro Research Article: Methods/New Tools Stroke is one of the main causes of death and disability worldwide. Over the past decades, several animal models of focal cerebral ischemia have been developed allowing to investigate pathophysiological mechanisms underlying stroke progression. Despite intense preclinical research efforts, the need for noninvasive mouse models of vascular occlusion targeting the middle cerebral artery yet avoiding mechanical intervention is still pressing. Here, by applying the photothrombotic stroke model to the distal branch of the middle cerebral artery, we developed a novel strategy to induce a targeted occlusion of a large blood vessel in mice. This approach induces unilateral damage encompassing most of the dorsal cortex from the motor up to the visual regions 1 week after stroke. Pronounced limb dystonia one day after the damage is partially recovered after one week. Furthermore, we observe the insurgence of blood vessel leakage and edema formation in the peri-infarct area. Finally, this model elicits a notable inflammatory response revealed as a strong increase in astrocyte density and morphologic complexity in the perilesional region of the cortex compared with both other regions of the ipsilesional and contralesional hemispheres, and in sham-operated mice. To conclude, the stroke model we developed induces in mice the light-mediated occlusion of one of the main targets of human ischemic stroke, the middle cerebral artery, free from the limitations of commonly used preclinical models. Society for Neuroscience 2023-02-07 /pmc/articles/PMC9910575/ /pubmed/36650068 http://dx.doi.org/10.1523/ENEURO.0244-22.2022 Text en Copyright © 2023 Conti et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article: Methods/New Tools Conti, Emilia Carlini, Noemi Piccardi, Benedetta Allegra Mascaro, Anna Letizia Pavone, Francesco Saverio Photothrombotic Middle Cerebral Artery Occlusion in Mice: A Novel Model of Ischemic Stroke |
title | Photothrombotic Middle Cerebral Artery Occlusion in Mice: A Novel Model of Ischemic Stroke |
title_full | Photothrombotic Middle Cerebral Artery Occlusion in Mice: A Novel Model of Ischemic Stroke |
title_fullStr | Photothrombotic Middle Cerebral Artery Occlusion in Mice: A Novel Model of Ischemic Stroke |
title_full_unstemmed | Photothrombotic Middle Cerebral Artery Occlusion in Mice: A Novel Model of Ischemic Stroke |
title_short | Photothrombotic Middle Cerebral Artery Occlusion in Mice: A Novel Model of Ischemic Stroke |
title_sort | photothrombotic middle cerebral artery occlusion in mice: a novel model of ischemic stroke |
topic | Research Article: Methods/New Tools |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910575/ https://www.ncbi.nlm.nih.gov/pubmed/36650068 http://dx.doi.org/10.1523/ENEURO.0244-22.2022 |
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