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Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene

Acute appendicitis represents one of the most common causes of emergency abdominal surgery worldwide. Meanwhile, Enterobius vermicularis has been suggested as one of the probable causes of appendicitis. In this study, the morphological characteristics of the remnant pinworms and pathologic changes w...

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Autores principales: Haghshenas, Maryam, Koosha, Mona, Latifi, Alireza, Kazemirad, Elham, Dehghan, Arash, Nikmanesh, Bahram, Mowlavi, Gholamreza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910638/
https://www.ncbi.nlm.nih.gov/pubmed/36758053
http://dx.doi.org/10.1371/journal.pone.0281622
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author Haghshenas, Maryam
Koosha, Mona
Latifi, Alireza
Kazemirad, Elham
Dehghan, Arash
Nikmanesh, Bahram
Mowlavi, Gholamreza
author_facet Haghshenas, Maryam
Koosha, Mona
Latifi, Alireza
Kazemirad, Elham
Dehghan, Arash
Nikmanesh, Bahram
Mowlavi, Gholamreza
author_sort Haghshenas, Maryam
collection PubMed
description Acute appendicitis represents one of the most common causes of emergency abdominal surgery worldwide. Meanwhile, Enterobius vermicularis has been suggested as one of the probable causes of appendicitis. In this study, the morphological characteristics of the remnant pinworms and pathologic changes were explored in old-archived FFPE tissues of appendectomies. Moreover, we provide the first molecular identification, genetic, and haplotype variation of this nematode from the old-archived FFPE tissue section of appendectomy using the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. Seventeen FFPE appendectomies with E. vermicularis infection, stored over 12–22 years, were collected from two different geographical areas of Iran. In the histopathological examination, tissue changes were observed in thirteen cases (76.4%) and inflammation in four blocks (23.5%). After DNA extraction, the cox1 gene was amplified in twelve (70.6%) cases using the nested polymerase chain reaction (PCR). Phylogenetic analysis and a median-joining network of 78 available cox1 sequences of E. vermicularis revealed 59 haplotypes. We identified five haplotypes that fell into type B. All Haplotypes are novel except for two haplotypes, Hap32 and Hap37, identical to E. vermicularis sequences from Iran, Greece, and Germany. The ranges of diversity distance and haplotype diversity within the isolates were 0–1.9% and HD:0.643–0.667, subsequently. Overall, the absence of inflammation or even tissue changes in some sections can suggest the possible non-inflammatory role of E. vermicularis in appendicitis. Although FFPE material suffers from PCR inhibition, we could successfully use nested PCR to characterize E. vermicularis in old-archived appendectomy blocks and suggest this method as a complementary diagnosis technique in pathology. While the predominant type was B in the Middle East and Europe, further studies on a larger sample size from different geographical regions could probably confirm the results obtained in the present study.
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spelling pubmed-99106382023-02-10 Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene Haghshenas, Maryam Koosha, Mona Latifi, Alireza Kazemirad, Elham Dehghan, Arash Nikmanesh, Bahram Mowlavi, Gholamreza PLoS One Research Article Acute appendicitis represents one of the most common causes of emergency abdominal surgery worldwide. Meanwhile, Enterobius vermicularis has been suggested as one of the probable causes of appendicitis. In this study, the morphological characteristics of the remnant pinworms and pathologic changes were explored in old-archived FFPE tissues of appendectomies. Moreover, we provide the first molecular identification, genetic, and haplotype variation of this nematode from the old-archived FFPE tissue section of appendectomy using the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. Seventeen FFPE appendectomies with E. vermicularis infection, stored over 12–22 years, were collected from two different geographical areas of Iran. In the histopathological examination, tissue changes were observed in thirteen cases (76.4%) and inflammation in four blocks (23.5%). After DNA extraction, the cox1 gene was amplified in twelve (70.6%) cases using the nested polymerase chain reaction (PCR). Phylogenetic analysis and a median-joining network of 78 available cox1 sequences of E. vermicularis revealed 59 haplotypes. We identified five haplotypes that fell into type B. All Haplotypes are novel except for two haplotypes, Hap32 and Hap37, identical to E. vermicularis sequences from Iran, Greece, and Germany. The ranges of diversity distance and haplotype diversity within the isolates were 0–1.9% and HD:0.643–0.667, subsequently. Overall, the absence of inflammation or even tissue changes in some sections can suggest the possible non-inflammatory role of E. vermicularis in appendicitis. Although FFPE material suffers from PCR inhibition, we could successfully use nested PCR to characterize E. vermicularis in old-archived appendectomy blocks and suggest this method as a complementary diagnosis technique in pathology. While the predominant type was B in the Middle East and Europe, further studies on a larger sample size from different geographical regions could probably confirm the results obtained in the present study. Public Library of Science 2023-02-09 /pmc/articles/PMC9910638/ /pubmed/36758053 http://dx.doi.org/10.1371/journal.pone.0281622 Text en © 2023 Haghshenas et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Haghshenas, Maryam
Koosha, Mona
Latifi, Alireza
Kazemirad, Elham
Dehghan, Arash
Nikmanesh, Bahram
Mowlavi, Gholamreza
Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene
title Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene
title_full Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene
title_fullStr Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene
title_full_unstemmed Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene
title_short Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene
title_sort detection of enterobius vermicularis in archived formalin-fixed paraffin-embedded (ffpe) appendectomy blocks: it’s potential to compare genetic variations based on mitochondrial dna (cox1) gene
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910638/
https://www.ncbi.nlm.nih.gov/pubmed/36758053
http://dx.doi.org/10.1371/journal.pone.0281622
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