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Effects of footshock stress on social behavior and neuronal activation in the medial prefrontal cortex and amygdala of male and female mice

Social behavior is complex and fundamental, and its deficits are common pathological features for several psychiatric disorders including anxiety, depression, and posttraumatic stress disorder. Acute stress may have a negative impact on social behavior, and these effects can vary based on sex. The a...

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Autores principales: Dorofeikova, Mariia, Borkar, Chandrashekhar D., Weissmuller, Katherine, Smith-Osborne, Lydia, Basavanhalli, Samhita, Bean, Erin, Smith, Avery, Duong, Anh, Resendez, Alexis, Fadok, Jonathan P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910713/
https://www.ncbi.nlm.nih.gov/pubmed/36757923
http://dx.doi.org/10.1371/journal.pone.0281388
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author Dorofeikova, Mariia
Borkar, Chandrashekhar D.
Weissmuller, Katherine
Smith-Osborne, Lydia
Basavanhalli, Samhita
Bean, Erin
Smith, Avery
Duong, Anh
Resendez, Alexis
Fadok, Jonathan P.
author_facet Dorofeikova, Mariia
Borkar, Chandrashekhar D.
Weissmuller, Katherine
Smith-Osborne, Lydia
Basavanhalli, Samhita
Bean, Erin
Smith, Avery
Duong, Anh
Resendez, Alexis
Fadok, Jonathan P.
author_sort Dorofeikova, Mariia
collection PubMed
description Social behavior is complex and fundamental, and its deficits are common pathological features for several psychiatric disorders including anxiety, depression, and posttraumatic stress disorder. Acute stress may have a negative impact on social behavior, and these effects can vary based on sex. The aim of this study was to explore the effect of acute footshock stress, using analogous parameters to those commonly used in fear conditioning assays, on the sociability of male and female C57BL/6J mice in a standard social approach test. Animals were divided into two main groups of footshock stress (22 male, 24 female) and context exposed control (23 male and 22 female). Each group had mice that were treated intraperitoneally with either the benzodiazepine—alprazolam (control: 10 male, 10 female; stress: 11 male, 11 female), or vehicle (control: 13 male, 12 female; stress: 11 male, 13 female). In all groups, neuronal activation during social approach was assessed using immunohistochemistry against the immediate early gene product cFos. Although footshock stress did not significantly alter sociability or latency to approach a social stimulus, it did increase defensive tail-rattling behavior specifically in males (p = 0.0022). This stress-induced increase in tail-rattling was alleviated by alprazolam (p = 0.03), yet alprazolam had no effect on female tail-rattling behavior in the stress group. Alprazolam lowered cFos expression in the medial prefrontal cortex (p = 0.001 infralimbic area, p = 0.02 prelimbic area), and social approach induced sex-dependent differences in cFos activation in the ventromedial intercalated cell clusters (p = 0.04). Social approach following stress-induced cFos expression was positively correlated with latency to approach and negatively correlated with sociability in the prelimbic area and multiple amygdala subregions (all p < 0.05). Collectively, our results suggest that acute footshock stress induces sex-dependent alterations in defensiveness and differential patterns of cFos activation during social approach.
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spelling pubmed-99107132023-02-10 Effects of footshock stress on social behavior and neuronal activation in the medial prefrontal cortex and amygdala of male and female mice Dorofeikova, Mariia Borkar, Chandrashekhar D. Weissmuller, Katherine Smith-Osborne, Lydia Basavanhalli, Samhita Bean, Erin Smith, Avery Duong, Anh Resendez, Alexis Fadok, Jonathan P. PLoS One Research Article Social behavior is complex and fundamental, and its deficits are common pathological features for several psychiatric disorders including anxiety, depression, and posttraumatic stress disorder. Acute stress may have a negative impact on social behavior, and these effects can vary based on sex. The aim of this study was to explore the effect of acute footshock stress, using analogous parameters to those commonly used in fear conditioning assays, on the sociability of male and female C57BL/6J mice in a standard social approach test. Animals were divided into two main groups of footshock stress (22 male, 24 female) and context exposed control (23 male and 22 female). Each group had mice that were treated intraperitoneally with either the benzodiazepine—alprazolam (control: 10 male, 10 female; stress: 11 male, 11 female), or vehicle (control: 13 male, 12 female; stress: 11 male, 13 female). In all groups, neuronal activation during social approach was assessed using immunohistochemistry against the immediate early gene product cFos. Although footshock stress did not significantly alter sociability or latency to approach a social stimulus, it did increase defensive tail-rattling behavior specifically in males (p = 0.0022). This stress-induced increase in tail-rattling was alleviated by alprazolam (p = 0.03), yet alprazolam had no effect on female tail-rattling behavior in the stress group. Alprazolam lowered cFos expression in the medial prefrontal cortex (p = 0.001 infralimbic area, p = 0.02 prelimbic area), and social approach induced sex-dependent differences in cFos activation in the ventromedial intercalated cell clusters (p = 0.04). Social approach following stress-induced cFos expression was positively correlated with latency to approach and negatively correlated with sociability in the prelimbic area and multiple amygdala subregions (all p < 0.05). Collectively, our results suggest that acute footshock stress induces sex-dependent alterations in defensiveness and differential patterns of cFos activation during social approach. Public Library of Science 2023-02-09 /pmc/articles/PMC9910713/ /pubmed/36757923 http://dx.doi.org/10.1371/journal.pone.0281388 Text en © 2023 Dorofeikova et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dorofeikova, Mariia
Borkar, Chandrashekhar D.
Weissmuller, Katherine
Smith-Osborne, Lydia
Basavanhalli, Samhita
Bean, Erin
Smith, Avery
Duong, Anh
Resendez, Alexis
Fadok, Jonathan P.
Effects of footshock stress on social behavior and neuronal activation in the medial prefrontal cortex and amygdala of male and female mice
title Effects of footshock stress on social behavior and neuronal activation in the medial prefrontal cortex and amygdala of male and female mice
title_full Effects of footshock stress on social behavior and neuronal activation in the medial prefrontal cortex and amygdala of male and female mice
title_fullStr Effects of footshock stress on social behavior and neuronal activation in the medial prefrontal cortex and amygdala of male and female mice
title_full_unstemmed Effects of footshock stress on social behavior and neuronal activation in the medial prefrontal cortex and amygdala of male and female mice
title_short Effects of footshock stress on social behavior and neuronal activation in the medial prefrontal cortex and amygdala of male and female mice
title_sort effects of footshock stress on social behavior and neuronal activation in the medial prefrontal cortex and amygdala of male and female mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910713/
https://www.ncbi.nlm.nih.gov/pubmed/36757923
http://dx.doi.org/10.1371/journal.pone.0281388
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