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Association of gut microbiota and SCFAs with finishing weight of Diannan small ear pigs

Finishing weight is a key economic trait in the domestic pig industry. Evidence has linked the gut microbiota and SCFAs to health and production performance in pigs. Nevertheless, for Diannan small ear (DSE) pigs, a specific pig breed in China, the potential effect of gut microbiota and SCFAs on the...

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Autores principales: Lan, Qun, Lian, Yuju, Peng, Peiya, Yang, Long, Zhao, Heng, Huang, Peng, Ma, Haiming, Wei, Hongjiang, Yin, Yulong, Liu, Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911695/
https://www.ncbi.nlm.nih.gov/pubmed/36778880
http://dx.doi.org/10.3389/fmicb.2023.1117965
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author Lan, Qun
Lian, Yuju
Peng, Peiya
Yang, Long
Zhao, Heng
Huang, Peng
Ma, Haiming
Wei, Hongjiang
Yin, Yulong
Liu, Mei
author_facet Lan, Qun
Lian, Yuju
Peng, Peiya
Yang, Long
Zhao, Heng
Huang, Peng
Ma, Haiming
Wei, Hongjiang
Yin, Yulong
Liu, Mei
author_sort Lan, Qun
collection PubMed
description Finishing weight is a key economic trait in the domestic pig industry. Evidence has linked the gut microbiota and SCFAs to health and production performance in pigs. Nevertheless, for Diannan small ear (DSE) pigs, a specific pig breed in China, the potential effect of gut microbiota and SCFAs on their finishing weight remains unclear. Herein, based on the data of the 16S ribosomal RNA gene and metagenomic sequencing analysis, we found that 13 OTUs could be potential biomarkers and 19 microbial species were associated with finishing weight. Among these, carbohydrate-decomposing bacteria of the families Streptococcaceae, Lactobacillaceae, and Prevotellaceae were positively related to finishing weight, whereas the microbial taxa associated with intestinal inflammation and damage exhibited opposite effects. In addition, interactions of these microbial species were found to be linked with finishing weight for the first time. Gut microbial functional annotation analysis indicated that CAZymes, such as glucosidase and glucanase could significantly affect finishing weight, given their roles in increasing nutrient absorption efficiency. Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthologies (KOs) and KEGG pathways analysis indicated that glycolysis/gluconeogenesis, phosphotransferase system (PTS), secondary bile acid biosynthesis, ABC transporters, sulfur metabolism, and one carbon pool by folate could act as key factors in regulating finishing weight. Additionally, SCFA levels, especially acetate and butyrate, had pivotal impacts on finishing weight. Finishing weight-associated species Prevotella sp. RS2, Ruminococcus sp. AF31-14BH and Lactobacillus pontis showed positive associations with butyrate concentration, and Paraprevotella xylaniphila and Bacteroides sp. OF04-15BH were positively related to acetate level. Taken together, our study provides essential knowledge for manipulating gut microbiomes to improve finishing weight. The underlying mechanisms of how gut microbiome and SCFAs modulate pigs’ finishing weight required further elucidation.
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spelling pubmed-99116952023-02-11 Association of gut microbiota and SCFAs with finishing weight of Diannan small ear pigs Lan, Qun Lian, Yuju Peng, Peiya Yang, Long Zhao, Heng Huang, Peng Ma, Haiming Wei, Hongjiang Yin, Yulong Liu, Mei Front Microbiol Microbiology Finishing weight is a key economic trait in the domestic pig industry. Evidence has linked the gut microbiota and SCFAs to health and production performance in pigs. Nevertheless, for Diannan small ear (DSE) pigs, a specific pig breed in China, the potential effect of gut microbiota and SCFAs on their finishing weight remains unclear. Herein, based on the data of the 16S ribosomal RNA gene and metagenomic sequencing analysis, we found that 13 OTUs could be potential biomarkers and 19 microbial species were associated with finishing weight. Among these, carbohydrate-decomposing bacteria of the families Streptococcaceae, Lactobacillaceae, and Prevotellaceae were positively related to finishing weight, whereas the microbial taxa associated with intestinal inflammation and damage exhibited opposite effects. In addition, interactions of these microbial species were found to be linked with finishing weight for the first time. Gut microbial functional annotation analysis indicated that CAZymes, such as glucosidase and glucanase could significantly affect finishing weight, given their roles in increasing nutrient absorption efficiency. Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthologies (KOs) and KEGG pathways analysis indicated that glycolysis/gluconeogenesis, phosphotransferase system (PTS), secondary bile acid biosynthesis, ABC transporters, sulfur metabolism, and one carbon pool by folate could act as key factors in regulating finishing weight. Additionally, SCFA levels, especially acetate and butyrate, had pivotal impacts on finishing weight. Finishing weight-associated species Prevotella sp. RS2, Ruminococcus sp. AF31-14BH and Lactobacillus pontis showed positive associations with butyrate concentration, and Paraprevotella xylaniphila and Bacteroides sp. OF04-15BH were positively related to acetate level. Taken together, our study provides essential knowledge for manipulating gut microbiomes to improve finishing weight. The underlying mechanisms of how gut microbiome and SCFAs modulate pigs’ finishing weight required further elucidation. Frontiers Media S.A. 2023-01-27 /pmc/articles/PMC9911695/ /pubmed/36778880 http://dx.doi.org/10.3389/fmicb.2023.1117965 Text en Copyright © 2023 Lan, Lian, Peng, Yang, Zhao, Huang, Ma, Wei, Yin and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lan, Qun
Lian, Yuju
Peng, Peiya
Yang, Long
Zhao, Heng
Huang, Peng
Ma, Haiming
Wei, Hongjiang
Yin, Yulong
Liu, Mei
Association of gut microbiota and SCFAs with finishing weight of Diannan small ear pigs
title Association of gut microbiota and SCFAs with finishing weight of Diannan small ear pigs
title_full Association of gut microbiota and SCFAs with finishing weight of Diannan small ear pigs
title_fullStr Association of gut microbiota and SCFAs with finishing weight of Diannan small ear pigs
title_full_unstemmed Association of gut microbiota and SCFAs with finishing weight of Diannan small ear pigs
title_short Association of gut microbiota and SCFAs with finishing weight of Diannan small ear pigs
title_sort association of gut microbiota and scfas with finishing weight of diannan small ear pigs
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911695/
https://www.ncbi.nlm.nih.gov/pubmed/36778880
http://dx.doi.org/10.3389/fmicb.2023.1117965
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