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Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models

Plastic particles in the nanometer range–called nanoplastics–are environmental contaminants with growing public health concern. As plastic particles are present in water, soil, air and food, human exposure via intestine and lung is unavoidable, but possible health effects are still to be elucidated....

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Autores principales: Busch, Mathias, Brouwer, Hugo, Aalderink, Germaine, Bredeck, Gerrit, Kämpfer, Angela A. M., Schins, Roel P. F., Bouwmeester, Hans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911716/
https://www.ncbi.nlm.nih.gov/pubmed/36777263
http://dx.doi.org/10.3389/ftox.2023.1112212
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author Busch, Mathias
Brouwer, Hugo
Aalderink, Germaine
Bredeck, Gerrit
Kämpfer, Angela A. M.
Schins, Roel P. F.
Bouwmeester, Hans
author_facet Busch, Mathias
Brouwer, Hugo
Aalderink, Germaine
Bredeck, Gerrit
Kämpfer, Angela A. M.
Schins, Roel P. F.
Bouwmeester, Hans
author_sort Busch, Mathias
collection PubMed
description Plastic particles in the nanometer range–called nanoplastics–are environmental contaminants with growing public health concern. As plastic particles are present in water, soil, air and food, human exposure via intestine and lung is unavoidable, but possible health effects are still to be elucidated. To better understand the Mode of Action of plastic particles, it is key to use experimental models that best reflect human physiology. Novel assessment methods like advanced cell models and several alternative approaches are currently used and developed in the scientific community. So far, the use of cancer cell line-based models is the standard approach regarding in vitro nanotoxicology. However, among the many advantages of the use of cancer cell lines, there are also disadvantages that might favor other approaches. In this review, we compare cell line-based models with stem cell-based in vitro models of the human intestine and lung. In the context of nanoplastics research, we highlight the advantages that come with the use of stem cells. Further, the specific challenges of testing nanoplastics in vitro are discussed. Although the use of stem cell-based models can be demanding, we conclude that, depending on the research question, stem cells in combination with advanced exposure strategies might be a more suitable approach than cancer cell lines when it comes to toxicological investigation of nanoplastics.
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spelling pubmed-99117162023-02-11 Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models Busch, Mathias Brouwer, Hugo Aalderink, Germaine Bredeck, Gerrit Kämpfer, Angela A. M. Schins, Roel P. F. Bouwmeester, Hans Front Toxicol Toxicology Plastic particles in the nanometer range–called nanoplastics–are environmental contaminants with growing public health concern. As plastic particles are present in water, soil, air and food, human exposure via intestine and lung is unavoidable, but possible health effects are still to be elucidated. To better understand the Mode of Action of plastic particles, it is key to use experimental models that best reflect human physiology. Novel assessment methods like advanced cell models and several alternative approaches are currently used and developed in the scientific community. So far, the use of cancer cell line-based models is the standard approach regarding in vitro nanotoxicology. However, among the many advantages of the use of cancer cell lines, there are also disadvantages that might favor other approaches. In this review, we compare cell line-based models with stem cell-based in vitro models of the human intestine and lung. In the context of nanoplastics research, we highlight the advantages that come with the use of stem cells. Further, the specific challenges of testing nanoplastics in vitro are discussed. Although the use of stem cell-based models can be demanding, we conclude that, depending on the research question, stem cells in combination with advanced exposure strategies might be a more suitable approach than cancer cell lines when it comes to toxicological investigation of nanoplastics. Frontiers Media S.A. 2023-01-27 /pmc/articles/PMC9911716/ /pubmed/36777263 http://dx.doi.org/10.3389/ftox.2023.1112212 Text en Copyright © 2023 Busch, Brouwer, Aalderink, Bredeck, Kämpfer, Schins and Bouwmeester. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Toxicology
Busch, Mathias
Brouwer, Hugo
Aalderink, Germaine
Bredeck, Gerrit
Kämpfer, Angela A. M.
Schins, Roel P. F.
Bouwmeester, Hans
Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models
title Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models
title_full Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models
title_fullStr Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models
title_full_unstemmed Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models
title_short Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models
title_sort investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models
topic Toxicology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911716/
https://www.ncbi.nlm.nih.gov/pubmed/36777263
http://dx.doi.org/10.3389/ftox.2023.1112212
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