Cargando…
Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models
Plastic particles in the nanometer range–called nanoplastics–are environmental contaminants with growing public health concern. As plastic particles are present in water, soil, air and food, human exposure via intestine and lung is unavoidable, but possible health effects are still to be elucidated....
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911716/ https://www.ncbi.nlm.nih.gov/pubmed/36777263 http://dx.doi.org/10.3389/ftox.2023.1112212 |
_version_ | 1784885049021169664 |
---|---|
author | Busch, Mathias Brouwer, Hugo Aalderink, Germaine Bredeck, Gerrit Kämpfer, Angela A. M. Schins, Roel P. F. Bouwmeester, Hans |
author_facet | Busch, Mathias Brouwer, Hugo Aalderink, Germaine Bredeck, Gerrit Kämpfer, Angela A. M. Schins, Roel P. F. Bouwmeester, Hans |
author_sort | Busch, Mathias |
collection | PubMed |
description | Plastic particles in the nanometer range–called nanoplastics–are environmental contaminants with growing public health concern. As plastic particles are present in water, soil, air and food, human exposure via intestine and lung is unavoidable, but possible health effects are still to be elucidated. To better understand the Mode of Action of plastic particles, it is key to use experimental models that best reflect human physiology. Novel assessment methods like advanced cell models and several alternative approaches are currently used and developed in the scientific community. So far, the use of cancer cell line-based models is the standard approach regarding in vitro nanotoxicology. However, among the many advantages of the use of cancer cell lines, there are also disadvantages that might favor other approaches. In this review, we compare cell line-based models with stem cell-based in vitro models of the human intestine and lung. In the context of nanoplastics research, we highlight the advantages that come with the use of stem cells. Further, the specific challenges of testing nanoplastics in vitro are discussed. Although the use of stem cell-based models can be demanding, we conclude that, depending on the research question, stem cells in combination with advanced exposure strategies might be a more suitable approach than cancer cell lines when it comes to toxicological investigation of nanoplastics. |
format | Online Article Text |
id | pubmed-9911716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99117162023-02-11 Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models Busch, Mathias Brouwer, Hugo Aalderink, Germaine Bredeck, Gerrit Kämpfer, Angela A. M. Schins, Roel P. F. Bouwmeester, Hans Front Toxicol Toxicology Plastic particles in the nanometer range–called nanoplastics–are environmental contaminants with growing public health concern. As plastic particles are present in water, soil, air and food, human exposure via intestine and lung is unavoidable, but possible health effects are still to be elucidated. To better understand the Mode of Action of plastic particles, it is key to use experimental models that best reflect human physiology. Novel assessment methods like advanced cell models and several alternative approaches are currently used and developed in the scientific community. So far, the use of cancer cell line-based models is the standard approach regarding in vitro nanotoxicology. However, among the many advantages of the use of cancer cell lines, there are also disadvantages that might favor other approaches. In this review, we compare cell line-based models with stem cell-based in vitro models of the human intestine and lung. In the context of nanoplastics research, we highlight the advantages that come with the use of stem cells. Further, the specific challenges of testing nanoplastics in vitro are discussed. Although the use of stem cell-based models can be demanding, we conclude that, depending on the research question, stem cells in combination with advanced exposure strategies might be a more suitable approach than cancer cell lines when it comes to toxicological investigation of nanoplastics. Frontiers Media S.A. 2023-01-27 /pmc/articles/PMC9911716/ /pubmed/36777263 http://dx.doi.org/10.3389/ftox.2023.1112212 Text en Copyright © 2023 Busch, Brouwer, Aalderink, Bredeck, Kämpfer, Schins and Bouwmeester. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Toxicology Busch, Mathias Brouwer, Hugo Aalderink, Germaine Bredeck, Gerrit Kämpfer, Angela A. M. Schins, Roel P. F. Bouwmeester, Hans Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models |
title | Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models |
title_full | Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models |
title_fullStr | Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models |
title_full_unstemmed | Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models |
title_short | Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models |
title_sort | investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models |
topic | Toxicology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9911716/ https://www.ncbi.nlm.nih.gov/pubmed/36777263 http://dx.doi.org/10.3389/ftox.2023.1112212 |
work_keys_str_mv | AT buschmathias investigatingnanoplasticstoxicityusingadvancedstemcellbasedintestinalandlunginvitromodels AT brouwerhugo investigatingnanoplasticstoxicityusingadvancedstemcellbasedintestinalandlunginvitromodels AT aalderinkgermaine investigatingnanoplasticstoxicityusingadvancedstemcellbasedintestinalandlunginvitromodels AT bredeckgerrit investigatingnanoplasticstoxicityusingadvancedstemcellbasedintestinalandlunginvitromodels AT kampferangelaam investigatingnanoplasticstoxicityusingadvancedstemcellbasedintestinalandlunginvitromodels AT schinsroelpf investigatingnanoplasticstoxicityusingadvancedstemcellbasedintestinalandlunginvitromodels AT bouwmeesterhans investigatingnanoplasticstoxicityusingadvancedstemcellbasedintestinalandlunginvitromodels |