The 3D‐structure, kinetics and dynamics of the E. coli nitroreductase NfsA with NADP (+) provide glimpses of its catalytic mechanism

Nitroreductases activate nitroaromatic antibiotics and cancer prodrugs to cytotoxic hydroxylamines and reduce quinones to quinols. Using steady‐state and stopped‐flow kinetics, we show that the Escherichia coli nitroreductase NfsA is 20–50 fold more active with NADPH than with NADH and that product...

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Detalles Bibliográficos
Autores principales: White, Scott A., Christofferson, Andrew J., Grainger, Alastair I., Day, Martin A., Jarrom, David, Graziano, Antonio E., Searle, Peter F., Hyde, Eva I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9912195/
https://www.ncbi.nlm.nih.gov/pubmed/35648111
http://dx.doi.org/10.1002/1873-3468.14413

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9912195/
https://www.ncbi.nlm.nih.gov/pubmed/35648111
http://dx.doi.org/10.1002/1873-3468.14413

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