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The 3D‐structure, kinetics and dynamics of the E. coli nitroreductase NfsA with NADP (+) provide glimpses of its catalytic mechanism
Nitroreductases activate nitroaromatic antibiotics and cancer prodrugs to cytotoxic hydroxylamines and reduce quinones to quinols. Using steady‐state and stopped‐flow kinetics, we show that the Escherichia coli nitroreductase NfsA is 20–50 fold more active with NADPH than with NADH and that product...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9912195/ https://www.ncbi.nlm.nih.gov/pubmed/35648111 http://dx.doi.org/10.1002/1873-3468.14413 |