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Metallothionein expression in feline injection site fibrosarcomas

BACKGROUND: Feline injection site fibrosarcoma is an aggressive and infiltrative tumour arising in the background of chronic inflammation. The aim of this study was to evaluate the expression of metallothionein (I-II) in feline injection site fibrosarcomas and to assess its possible relationships wi...

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Autores principales: Mikiewicz, Mateusz, Paździor-Czapula, Katarzyna, Fiedorowicz, Joanna, Gesek, Michał, Otrocka-Domagała, Iwona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9912506/
https://www.ncbi.nlm.nih.gov/pubmed/36759896
http://dx.doi.org/10.1186/s12917-023-03604-5
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author Mikiewicz, Mateusz
Paździor-Czapula, Katarzyna
Fiedorowicz, Joanna
Gesek, Michał
Otrocka-Domagała, Iwona
author_facet Mikiewicz, Mateusz
Paździor-Czapula, Katarzyna
Fiedorowicz, Joanna
Gesek, Michał
Otrocka-Domagała, Iwona
author_sort Mikiewicz, Mateusz
collection PubMed
description BACKGROUND: Feline injection site fibrosarcoma is an aggressive and infiltrative tumour arising in the background of chronic inflammation. The aim of this study was to evaluate the expression of metallothionein (I-II) in feline injection site fibrosarcomas and to assess its possible relationships with Ki67 index, inflammation score and tumour grade. The study included 40 feline fibrosarcomas, located in the common injection sites (i.e., interscapular area, thigh, flank), constituting archival diagnostic specimens collected between 2019–2020. Tumours were graded histologically according to the newly proposed soft-tissue sarcoma grading system in cats. Immunohistochemistry was performed to evaluate the expression of Ki67 and metallothionein in tumour cells. RESULTS: The cytoplasmic and sometimes nuclear expression of metallothionein was observed in all tumours grade I, 66.67% of tumours grade II and 55% of tumours grade III. The expression of metallothionein was negatively correlated with tumour grade and inflammation score, while the Ki67 index was positively correlated with tumour grade, inflammation score and necrosis score. CONCLUSION: The downregulation of MT expression in feline injection site fibrosarcomas seems to be connected with an increase in the inflammatory infiltration, hence tumour progression. This is the first study describing metallothionein expression in feline injection site fibrosarcomas. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-023-03604-5.
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spelling pubmed-99125062023-02-11 Metallothionein expression in feline injection site fibrosarcomas Mikiewicz, Mateusz Paździor-Czapula, Katarzyna Fiedorowicz, Joanna Gesek, Michał Otrocka-Domagała, Iwona BMC Vet Res Research BACKGROUND: Feline injection site fibrosarcoma is an aggressive and infiltrative tumour arising in the background of chronic inflammation. The aim of this study was to evaluate the expression of metallothionein (I-II) in feline injection site fibrosarcomas and to assess its possible relationships with Ki67 index, inflammation score and tumour grade. The study included 40 feline fibrosarcomas, located in the common injection sites (i.e., interscapular area, thigh, flank), constituting archival diagnostic specimens collected between 2019–2020. Tumours were graded histologically according to the newly proposed soft-tissue sarcoma grading system in cats. Immunohistochemistry was performed to evaluate the expression of Ki67 and metallothionein in tumour cells. RESULTS: The cytoplasmic and sometimes nuclear expression of metallothionein was observed in all tumours grade I, 66.67% of tumours grade II and 55% of tumours grade III. The expression of metallothionein was negatively correlated with tumour grade and inflammation score, while the Ki67 index was positively correlated with tumour grade, inflammation score and necrosis score. CONCLUSION: The downregulation of MT expression in feline injection site fibrosarcomas seems to be connected with an increase in the inflammatory infiltration, hence tumour progression. This is the first study describing metallothionein expression in feline injection site fibrosarcomas. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-023-03604-5. BioMed Central 2023-02-10 /pmc/articles/PMC9912506/ /pubmed/36759896 http://dx.doi.org/10.1186/s12917-023-03604-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mikiewicz, Mateusz
Paździor-Czapula, Katarzyna
Fiedorowicz, Joanna
Gesek, Michał
Otrocka-Domagała, Iwona
Metallothionein expression in feline injection site fibrosarcomas
title Metallothionein expression in feline injection site fibrosarcomas
title_full Metallothionein expression in feline injection site fibrosarcomas
title_fullStr Metallothionein expression in feline injection site fibrosarcomas
title_full_unstemmed Metallothionein expression in feline injection site fibrosarcomas
title_short Metallothionein expression in feline injection site fibrosarcomas
title_sort metallothionein expression in feline injection site fibrosarcomas
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9912506/
https://www.ncbi.nlm.nih.gov/pubmed/36759896
http://dx.doi.org/10.1186/s12917-023-03604-5
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