Cargando…
Dissecting the microbial community structure of internal organs during the early postmortem period in a murine corpse model
BACKGROUND: Microorganisms distribute and proliferate both inside and outside the body, which are the main mediators of decomposition after death. However, limited information is available on the postmortem microbiota changes of extraintestinal body sites in the early decomposition stage of mammalia...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9912631/ https://www.ncbi.nlm.nih.gov/pubmed/36765295 http://dx.doi.org/10.1186/s12866-023-02786-0 |
_version_ | 1784885246699765760 |
---|---|
author | Liu, Ruina Zhang, Kai Li, Huan Sun, Qinru Wei, Xin Li, Huiyu Zhang, Siruo Fan, Shuanliang Wang, Zhenyuan |
author_facet | Liu, Ruina Zhang, Kai Li, Huan Sun, Qinru Wei, Xin Li, Huiyu Zhang, Siruo Fan, Shuanliang Wang, Zhenyuan |
author_sort | Liu, Ruina |
collection | PubMed |
description | BACKGROUND: Microorganisms distribute and proliferate both inside and outside the body, which are the main mediators of decomposition after death. However, limited information is available on the postmortem microbiota changes of extraintestinal body sites in the early decomposition stage of mammalian corpses. RESULTS: This study investigated microbial composition variations among different organs and the relationship between microbial communities and time since death over 1 day of decomposition in male C57BL/6 J mice by 16S rRNA sequencing. During 1 day of decomposition, Agrobacterium, Prevotella, Bacillus, and Turicibacter were regarded as time-relevant genera in internal organs at different timepoints. Pathways associated with lipid, amino acid, carbohydrate and terpenoid and polyketide metabolism were significantly enriched at 8 h than that at 0.5 or 4 h. The microbiome compositions and postmortem metabolic pathways differed by time since death, and more importantly, these alterations were organ specific. CONCLUSION: The dominant microbes differed by organ, while they tended toward similarity as decomposition progressed. The observed thanatomicrobiome variation by body site provides new knowledge into decomposition ecology and forensic microbiology. Additionally, the microbes detected at 0.5 h in internal organs may inform a new direction for organ transplantation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-02786-0. |
format | Online Article Text |
id | pubmed-9912631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-99126312023-02-11 Dissecting the microbial community structure of internal organs during the early postmortem period in a murine corpse model Liu, Ruina Zhang, Kai Li, Huan Sun, Qinru Wei, Xin Li, Huiyu Zhang, Siruo Fan, Shuanliang Wang, Zhenyuan BMC Microbiol Research Article BACKGROUND: Microorganisms distribute and proliferate both inside and outside the body, which are the main mediators of decomposition after death. However, limited information is available on the postmortem microbiota changes of extraintestinal body sites in the early decomposition stage of mammalian corpses. RESULTS: This study investigated microbial composition variations among different organs and the relationship between microbial communities and time since death over 1 day of decomposition in male C57BL/6 J mice by 16S rRNA sequencing. During 1 day of decomposition, Agrobacterium, Prevotella, Bacillus, and Turicibacter were regarded as time-relevant genera in internal organs at different timepoints. Pathways associated with lipid, amino acid, carbohydrate and terpenoid and polyketide metabolism were significantly enriched at 8 h than that at 0.5 or 4 h. The microbiome compositions and postmortem metabolic pathways differed by time since death, and more importantly, these alterations were organ specific. CONCLUSION: The dominant microbes differed by organ, while they tended toward similarity as decomposition progressed. The observed thanatomicrobiome variation by body site provides new knowledge into decomposition ecology and forensic microbiology. Additionally, the microbes detected at 0.5 h in internal organs may inform a new direction for organ transplantation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-02786-0. BioMed Central 2023-02-10 /pmc/articles/PMC9912631/ /pubmed/36765295 http://dx.doi.org/10.1186/s12866-023-02786-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Liu, Ruina Zhang, Kai Li, Huan Sun, Qinru Wei, Xin Li, Huiyu Zhang, Siruo Fan, Shuanliang Wang, Zhenyuan Dissecting the microbial community structure of internal organs during the early postmortem period in a murine corpse model |
title | Dissecting the microbial community structure of internal organs during the early postmortem period in a murine corpse model |
title_full | Dissecting the microbial community structure of internal organs during the early postmortem period in a murine corpse model |
title_fullStr | Dissecting the microbial community structure of internal organs during the early postmortem period in a murine corpse model |
title_full_unstemmed | Dissecting the microbial community structure of internal organs during the early postmortem period in a murine corpse model |
title_short | Dissecting the microbial community structure of internal organs during the early postmortem period in a murine corpse model |
title_sort | dissecting the microbial community structure of internal organs during the early postmortem period in a murine corpse model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9912631/ https://www.ncbi.nlm.nih.gov/pubmed/36765295 http://dx.doi.org/10.1186/s12866-023-02786-0 |
work_keys_str_mv | AT liuruina dissectingthemicrobialcommunitystructureofinternalorgansduringtheearlypostmortemperiodinamurinecorpsemodel AT zhangkai dissectingthemicrobialcommunitystructureofinternalorgansduringtheearlypostmortemperiodinamurinecorpsemodel AT lihuan dissectingthemicrobialcommunitystructureofinternalorgansduringtheearlypostmortemperiodinamurinecorpsemodel AT sunqinru dissectingthemicrobialcommunitystructureofinternalorgansduringtheearlypostmortemperiodinamurinecorpsemodel AT weixin dissectingthemicrobialcommunitystructureofinternalorgansduringtheearlypostmortemperiodinamurinecorpsemodel AT lihuiyu dissectingthemicrobialcommunitystructureofinternalorgansduringtheearlypostmortemperiodinamurinecorpsemodel AT zhangsiruo dissectingthemicrobialcommunitystructureofinternalorgansduringtheearlypostmortemperiodinamurinecorpsemodel AT fanshuanliang dissectingthemicrobialcommunitystructureofinternalorgansduringtheearlypostmortemperiodinamurinecorpsemodel AT wangzhenyuan dissectingthemicrobialcommunitystructureofinternalorgansduringtheearlypostmortemperiodinamurinecorpsemodel |