Enzyme Feed Additive with Arazyme Improve Growth Performance, Meat Quality, and Gut Microbiome of Pigs

SIMPLE SUMMARY: Supplementation of exogenous enzymes in a diet, particularly protease as a part of an enzyme mixture, is widely used with the expectation that it will enhance the efficiency of nutrient utilization by neutralizing anti-nutritional factors and increasing nutrient digestibility and, thereby, improving growth performance. In our study, dietary supplementation of exogenous enzymes derived from invertebrate symbiotic bacteria affected the growth performance, meat quality, and gut microbiota of pigs. The results of this study suggest that applying exogenous enzymes derived from invertebrate symbiotic bacteria enhances animal performance and can be used as a theoretical basis for further studies. ABSTRACT: The supplementation of pig diets with exogenous enzymes is widely used with the expectation that it will improve the efficiency of nutrient utilization, thereby, improving growth performance. This study aims to evaluate the effects of a 0.1% (v/v) multi-enzyme (a mixture of arazyme (2,500,000 Unit/kg), xylanase (200,000 Unit/kg) and mannanase (200,000 Unit/kg)) supplementation derived from invertebrate symbiotic bacteria on pig performance. Here, 256 growing pigs were assigned to control and treatment groups, respectively. The treatment group exhibited a significantly reduced average slaughter age; the final body weight and average daily gain increased compared with that of the control group. In the treatment group, the longissimus muscle showed a remarkable decrease in cooking loss, shear force, and color values with increased essential and non-essential amino acid concentrations. Furthermore, the concentrations of mono- and polyunsaturated fatty acids in the treatment group increased. Feed additive supplementation increased the family of Ruminococcaceae and genera Lactobacillus, Limosilactobacillus, Turicibacter, and Oscillibacter, which play a positive role in the host physiology and health. Predicted metabolic pathway analysis confirmed that operational taxonomic units and predicted amino acid biosynthesis pathways were strongly associated. The results suggest that applying exogenous enzymes derived from invertebrate symbiotic bacteria enhances animal performance.