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Endocannabinoid 2-Arachidonoylglycerol Levels in the Anterior Cingulate Cortex, Caudate Putamen, Nucleus Accumbens, and Piriform Cortex Were Upregulated by Chronic Restraint Stress

Endocannabinoid 2-arachidonoylglycerol (2-AG) has been implicated in habituation to stress, and its augmentation reduces stress-induced anxiety-like behavior. Chronic restraint stress (CRS) changes the 2-AG levels in some gross brain areas, such as the forebrain. However, the detailed spatial distri...

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Autores principales: Zhai, Qing, Islam, Ariful, Chen, Bin, Zhang, Hengsen, Chi, Do Huu, Mamun, Md. Al, Takahashi, Yutaka, Sato, Noriko, Yamasue, Hidenori, Nakajima, Yoshiki, Nagashima, Yu, Sano, Fumito, Sato, Tomohito, Kahyo, Tomoaki, Setou, Mitsutoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913316/
https://www.ncbi.nlm.nih.gov/pubmed/36766735
http://dx.doi.org/10.3390/cells12030393
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author Zhai, Qing
Islam, Ariful
Chen, Bin
Zhang, Hengsen
Chi, Do Huu
Mamun, Md. Al
Takahashi, Yutaka
Sato, Noriko
Yamasue, Hidenori
Nakajima, Yoshiki
Nagashima, Yu
Sano, Fumito
Sato, Tomohito
Kahyo, Tomoaki
Setou, Mitsutoshi
author_facet Zhai, Qing
Islam, Ariful
Chen, Bin
Zhang, Hengsen
Chi, Do Huu
Mamun, Md. Al
Takahashi, Yutaka
Sato, Noriko
Yamasue, Hidenori
Nakajima, Yoshiki
Nagashima, Yu
Sano, Fumito
Sato, Tomohito
Kahyo, Tomoaki
Setou, Mitsutoshi
author_sort Zhai, Qing
collection PubMed
description Endocannabinoid 2-arachidonoylglycerol (2-AG) has been implicated in habituation to stress, and its augmentation reduces stress-induced anxiety-like behavior. Chronic restraint stress (CRS) changes the 2-AG levels in some gross brain areas, such as the forebrain. However, the detailed spatial distribution of 2-AG and its changes by CRS in stress processing-related anatomical structures such as the anterior cingulate cortex (ACC), caudate putamen (CP), nucleus accumbens (NAc), and piriform cortex (PIR) are still unclear. In this study, mice were restrained for 30 min in a 50 mL-centrifuge tube for eight consecutive days, followed by imaging of the coronal brain sections of control and stressed mice using desorption electrospray ionization mass spectrometry imaging (DESI-MSI). The results showed that from the forebrain to the cerebellum, 2-AG levels were highest in the hypothalamus and lowest in the hippocampal region. 2-AG levels were significantly (p < 0.05) upregulated and 2-AG precursors levels were significantly (p < 0.05) downregulated in the ACC, CP, NAc, and PIR of stressed mice compared with control mice. This study provided direct evidence of 2-AG expression and changes, suggesting that 2-AG levels are increased in the ACC CP, NAc, and PIR when individuals are under chronic stress.
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spelling pubmed-99133162023-02-11 Endocannabinoid 2-Arachidonoylglycerol Levels in the Anterior Cingulate Cortex, Caudate Putamen, Nucleus Accumbens, and Piriform Cortex Were Upregulated by Chronic Restraint Stress Zhai, Qing Islam, Ariful Chen, Bin Zhang, Hengsen Chi, Do Huu Mamun, Md. Al Takahashi, Yutaka Sato, Noriko Yamasue, Hidenori Nakajima, Yoshiki Nagashima, Yu Sano, Fumito Sato, Tomohito Kahyo, Tomoaki Setou, Mitsutoshi Cells Article Endocannabinoid 2-arachidonoylglycerol (2-AG) has been implicated in habituation to stress, and its augmentation reduces stress-induced anxiety-like behavior. Chronic restraint stress (CRS) changes the 2-AG levels in some gross brain areas, such as the forebrain. However, the detailed spatial distribution of 2-AG and its changes by CRS in stress processing-related anatomical structures such as the anterior cingulate cortex (ACC), caudate putamen (CP), nucleus accumbens (NAc), and piriform cortex (PIR) are still unclear. In this study, mice were restrained for 30 min in a 50 mL-centrifuge tube for eight consecutive days, followed by imaging of the coronal brain sections of control and stressed mice using desorption electrospray ionization mass spectrometry imaging (DESI-MSI). The results showed that from the forebrain to the cerebellum, 2-AG levels were highest in the hypothalamus and lowest in the hippocampal region. 2-AG levels were significantly (p < 0.05) upregulated and 2-AG precursors levels were significantly (p < 0.05) downregulated in the ACC, CP, NAc, and PIR of stressed mice compared with control mice. This study provided direct evidence of 2-AG expression and changes, suggesting that 2-AG levels are increased in the ACC CP, NAc, and PIR when individuals are under chronic stress. MDPI 2023-01-21 /pmc/articles/PMC9913316/ /pubmed/36766735 http://dx.doi.org/10.3390/cells12030393 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhai, Qing
Islam, Ariful
Chen, Bin
Zhang, Hengsen
Chi, Do Huu
Mamun, Md. Al
Takahashi, Yutaka
Sato, Noriko
Yamasue, Hidenori
Nakajima, Yoshiki
Nagashima, Yu
Sano, Fumito
Sato, Tomohito
Kahyo, Tomoaki
Setou, Mitsutoshi
Endocannabinoid 2-Arachidonoylglycerol Levels in the Anterior Cingulate Cortex, Caudate Putamen, Nucleus Accumbens, and Piriform Cortex Were Upregulated by Chronic Restraint Stress
title Endocannabinoid 2-Arachidonoylglycerol Levels in the Anterior Cingulate Cortex, Caudate Putamen, Nucleus Accumbens, and Piriform Cortex Were Upregulated by Chronic Restraint Stress
title_full Endocannabinoid 2-Arachidonoylglycerol Levels in the Anterior Cingulate Cortex, Caudate Putamen, Nucleus Accumbens, and Piriform Cortex Were Upregulated by Chronic Restraint Stress
title_fullStr Endocannabinoid 2-Arachidonoylglycerol Levels in the Anterior Cingulate Cortex, Caudate Putamen, Nucleus Accumbens, and Piriform Cortex Were Upregulated by Chronic Restraint Stress
title_full_unstemmed Endocannabinoid 2-Arachidonoylglycerol Levels in the Anterior Cingulate Cortex, Caudate Putamen, Nucleus Accumbens, and Piriform Cortex Were Upregulated by Chronic Restraint Stress
title_short Endocannabinoid 2-Arachidonoylglycerol Levels in the Anterior Cingulate Cortex, Caudate Putamen, Nucleus Accumbens, and Piriform Cortex Were Upregulated by Chronic Restraint Stress
title_sort endocannabinoid 2-arachidonoylglycerol levels in the anterior cingulate cortex, caudate putamen, nucleus accumbens, and piriform cortex were upregulated by chronic restraint stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913316/
https://www.ncbi.nlm.nih.gov/pubmed/36766735
http://dx.doi.org/10.3390/cells12030393
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