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Comparison of Radiation Response between 2D and 3D Cell Culture Models of Different Human Cancer Cell Lines

Radiation therapy is one of the most effective tools in cancer therapy. However, success varies individually, necessitating improved understanding of radiobiology. Three-dimensional (3D) tumor spheroids are increasingly gaining attention, being a superior in vitro cancer model compared to 2D cell cu...

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Autores principales: Raitanen, Julia, Barta, Bernadette, Hacker, Marcus, Georg, Dietmar, Balber, Theresa, Mitterhauser, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913494/
https://www.ncbi.nlm.nih.gov/pubmed/36766704
http://dx.doi.org/10.3390/cells12030360
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author Raitanen, Julia
Barta, Bernadette
Hacker, Marcus
Georg, Dietmar
Balber, Theresa
Mitterhauser, Markus
author_facet Raitanen, Julia
Barta, Bernadette
Hacker, Marcus
Georg, Dietmar
Balber, Theresa
Mitterhauser, Markus
author_sort Raitanen, Julia
collection PubMed
description Radiation therapy is one of the most effective tools in cancer therapy. However, success varies individually, necessitating improved understanding of radiobiology. Three-dimensional (3D) tumor spheroids are increasingly gaining attention, being a superior in vitro cancer model compared to 2D cell cultures. This in vitro study aimed at comparing radiation responses in 2D and 3D cell culture models of different human cancer cell lines (PC-3, LNCaP and T-47D) irradiated with varying doses (1, 2, 4, 6, 8 or 20 Gy) of X-ray beams. Radiation response was analyzed by growth analysis, various cell viability assays (e.g., clonogenic assay, resazurin assay) and amount of DNA damage (γH2AX Western Blot). Results showed decreasing cell proliferation with the increase of radiation doses for all cell lines in monolayers and spheroids of LNCaP and T-47D. However, significantly lower radiosensitivity was detected in spheroids, most pronounced in PC-3, evincing radiation resistance of PC-3 spheroids up to 8 Gy and significant growth inhibition only by a dose escalation of 20 Gy. Cell line comparison showed highest radiosensitivity in LNCaP, followed by T-47D and PC-3 in 2D, whereas, in 3D, T-47D showed highest sensitivity. The results substantiate the significant differences in radiobiological response to X-rays between 2D and 3D cell culture models.
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spelling pubmed-99134942023-02-11 Comparison of Radiation Response between 2D and 3D Cell Culture Models of Different Human Cancer Cell Lines Raitanen, Julia Barta, Bernadette Hacker, Marcus Georg, Dietmar Balber, Theresa Mitterhauser, Markus Cells Article Radiation therapy is one of the most effective tools in cancer therapy. However, success varies individually, necessitating improved understanding of radiobiology. Three-dimensional (3D) tumor spheroids are increasingly gaining attention, being a superior in vitro cancer model compared to 2D cell cultures. This in vitro study aimed at comparing radiation responses in 2D and 3D cell culture models of different human cancer cell lines (PC-3, LNCaP and T-47D) irradiated with varying doses (1, 2, 4, 6, 8 or 20 Gy) of X-ray beams. Radiation response was analyzed by growth analysis, various cell viability assays (e.g., clonogenic assay, resazurin assay) and amount of DNA damage (γH2AX Western Blot). Results showed decreasing cell proliferation with the increase of radiation doses for all cell lines in monolayers and spheroids of LNCaP and T-47D. However, significantly lower radiosensitivity was detected in spheroids, most pronounced in PC-3, evincing radiation resistance of PC-3 spheroids up to 8 Gy and significant growth inhibition only by a dose escalation of 20 Gy. Cell line comparison showed highest radiosensitivity in LNCaP, followed by T-47D and PC-3 in 2D, whereas, in 3D, T-47D showed highest sensitivity. The results substantiate the significant differences in radiobiological response to X-rays between 2D and 3D cell culture models. MDPI 2023-01-18 /pmc/articles/PMC9913494/ /pubmed/36766704 http://dx.doi.org/10.3390/cells12030360 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raitanen, Julia
Barta, Bernadette
Hacker, Marcus
Georg, Dietmar
Balber, Theresa
Mitterhauser, Markus
Comparison of Radiation Response between 2D and 3D Cell Culture Models of Different Human Cancer Cell Lines
title Comparison of Radiation Response between 2D and 3D Cell Culture Models of Different Human Cancer Cell Lines
title_full Comparison of Radiation Response between 2D and 3D Cell Culture Models of Different Human Cancer Cell Lines
title_fullStr Comparison of Radiation Response between 2D and 3D Cell Culture Models of Different Human Cancer Cell Lines
title_full_unstemmed Comparison of Radiation Response between 2D and 3D Cell Culture Models of Different Human Cancer Cell Lines
title_short Comparison of Radiation Response between 2D and 3D Cell Culture Models of Different Human Cancer Cell Lines
title_sort comparison of radiation response between 2d and 3d cell culture models of different human cancer cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913494/
https://www.ncbi.nlm.nih.gov/pubmed/36766704
http://dx.doi.org/10.3390/cells12030360
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