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Blood-Brain Barrier-Associated Proteins Are Elevated in Serum of Epilepsy Patients

Blood–brain barrier (BBB) dysfunction emerges as one of the mechanisms underlying the induction of seizures and epileptogenesis. There is growing evidence that seizures also affect BBB, yet only scarce data is available regarding serum levels of BBB-associated proteins in chronic epilepsy. In this s...

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Autores principales: Bronisz, Elżbieta, Cudna, Agnieszka, Wierzbicka, Aleksandra, Kurkowska-Jastrzębska, Iwona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913812/
https://www.ncbi.nlm.nih.gov/pubmed/36766708
http://dx.doi.org/10.3390/cells12030368
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author Bronisz, Elżbieta
Cudna, Agnieszka
Wierzbicka, Aleksandra
Kurkowska-Jastrzębska, Iwona
author_facet Bronisz, Elżbieta
Cudna, Agnieszka
Wierzbicka, Aleksandra
Kurkowska-Jastrzębska, Iwona
author_sort Bronisz, Elżbieta
collection PubMed
description Blood–brain barrier (BBB) dysfunction emerges as one of the mechanisms underlying the induction of seizures and epileptogenesis. There is growing evidence that seizures also affect BBB, yet only scarce data is available regarding serum levels of BBB-associated proteins in chronic epilepsy. In this study, we aimed to assess serum levels of molecules associated with BBB in patients with epilepsy in the interictal period. Serum levels of MMP-9, MMP-2, TIMP-1, TIMP-2, S100B, CCL-2, ICAM-1, P-selectin, and TSP-2 were examined in a group of 100 patients who were seizure-free for a minimum of seven days and analyzed by ELISA. The results were compared with an age- and sex-matched control group. Serum levels of MMP-9, MMP-2, TIMP-1, TIMP-2 and S100B were higher in patients with epilepsy in comparison to control group (p < 0.0001; <0.0001; 0.001; <0.0001; <0.0001, respectively). Levels of CCL-2, ICAM-1, P-selectin and TSP-2 did not differ between the two groups. Serum levels of MMP-9, MMP-2, TIMP-1, TIMP-2 and S100B are elevated in patients with epilepsy in the interictal period, which suggests chronic processes of BBB disruption and restoration. The pathological process initiating epilepsy, in addition to seizures, is probably the factor contributing to the elevation of serum levels of the examined molecules.
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spelling pubmed-99138122023-02-11 Blood-Brain Barrier-Associated Proteins Are Elevated in Serum of Epilepsy Patients Bronisz, Elżbieta Cudna, Agnieszka Wierzbicka, Aleksandra Kurkowska-Jastrzębska, Iwona Cells Article Blood–brain barrier (BBB) dysfunction emerges as one of the mechanisms underlying the induction of seizures and epileptogenesis. There is growing evidence that seizures also affect BBB, yet only scarce data is available regarding serum levels of BBB-associated proteins in chronic epilepsy. In this study, we aimed to assess serum levels of molecules associated with BBB in patients with epilepsy in the interictal period. Serum levels of MMP-9, MMP-2, TIMP-1, TIMP-2, S100B, CCL-2, ICAM-1, P-selectin, and TSP-2 were examined in a group of 100 patients who were seizure-free for a minimum of seven days and analyzed by ELISA. The results were compared with an age- and sex-matched control group. Serum levels of MMP-9, MMP-2, TIMP-1, TIMP-2 and S100B were higher in patients with epilepsy in comparison to control group (p < 0.0001; <0.0001; 0.001; <0.0001; <0.0001, respectively). Levels of CCL-2, ICAM-1, P-selectin and TSP-2 did not differ between the two groups. Serum levels of MMP-9, MMP-2, TIMP-1, TIMP-2 and S100B are elevated in patients with epilepsy in the interictal period, which suggests chronic processes of BBB disruption and restoration. The pathological process initiating epilepsy, in addition to seizures, is probably the factor contributing to the elevation of serum levels of the examined molecules. MDPI 2023-01-19 /pmc/articles/PMC9913812/ /pubmed/36766708 http://dx.doi.org/10.3390/cells12030368 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bronisz, Elżbieta
Cudna, Agnieszka
Wierzbicka, Aleksandra
Kurkowska-Jastrzębska, Iwona
Blood-Brain Barrier-Associated Proteins Are Elevated in Serum of Epilepsy Patients
title Blood-Brain Barrier-Associated Proteins Are Elevated in Serum of Epilepsy Patients
title_full Blood-Brain Barrier-Associated Proteins Are Elevated in Serum of Epilepsy Patients
title_fullStr Blood-Brain Barrier-Associated Proteins Are Elevated in Serum of Epilepsy Patients
title_full_unstemmed Blood-Brain Barrier-Associated Proteins Are Elevated in Serum of Epilepsy Patients
title_short Blood-Brain Barrier-Associated Proteins Are Elevated in Serum of Epilepsy Patients
title_sort blood-brain barrier-associated proteins are elevated in serum of epilepsy patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913812/
https://www.ncbi.nlm.nih.gov/pubmed/36766708
http://dx.doi.org/10.3390/cells12030368
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